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Plasmid#47801PurposeExpresses human NHERF2 in mammalian cellsDepositorInsertNHERF2 (NHERF2 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-14-Mfn1-T562A
Plasmid#191976PurposeTo produce mutants mimicking dephosphorylated Mfn1DepositorInsertMfn1 T562A (Mfn1 Mouse)
UseTagsExpressionMammalianMutationT562APromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-14-Mfn1-T562D
Plasmid#191977PurposeTo produce mutants mimicking phosphorylated Mfn1DepositorInsertMfn1 T562D (Mfn1 Mouse)
UseTagsExpressionMammalianMutationT562DPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSilencer2.1-U6-LRS1F50C
Plasmid#190856PurposeFor mammalian expression of LRS1F50C, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer is regulated by riboswitch elements.DepositorInsertLRS1F50C (A Dual-Specificity aptamer targeting OGT and GFP)
UseAffinity Reagent/ Antibody and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pENV8(GAAA)-GFPuv
Plasmid#70128PurposeNegative control cobalamin riboswitch reporter vector for use in E. coli; deletion of regulatory kissing loop interaction by conversion of L5 to GAAA tetra loopDepositorInsertkissing loop regulatory mutant of environmental cobalamin riboswitch sequence (env8)
UseSynthetic BiologyTagsNoneExpressionBacterialMutationPromotertacAvailable sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-CAML
Plasmid#22284DepositorInsertcalcium-modulating ligand (CAMLG Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ Lox 5'UTR
Plasmid#67932PurposeTranslational reporter - Lox 5'UTRDepositorInsertsfGFP-NLS-P2A-NLS-mCherry (LOX Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC-STITCH
Plasmid#129535PurposeSTITCH (Synthetic Toplogical Insulator with TetO for CHromatin-engineering) with homology arms for MYC locus insertion.DepositorInsertSTITCH
UseCRISPR; Donor templateTagsExpressionMutationPromoterAvailable sinceDec. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mSmo-NQ4-V5
Plasmid#126416PurposeLocalization, Expression of Smoothened NQ4 in mammalian cell lines.DepositorInsertm-Smoothened (Smo Mouse)
UseTagsExpressionMammalianMutationSmo glycosylation site NQ4PromoterAvailable sinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP eIF2beta
Plasmid#45902DepositorInserteIF2S2 (Eif2s2 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM1-C49,50,83,103A
Plasmid#58421PurposeExpresses murine IFITM1-C49,50,83,103A with an N-terminal HA tag in mammalian cells (cysteine-less mutant that is not palmitoylated).DepositorInsertIFITM1 (Ifitm1 Mouse)
UseTagsHAExpressionMammalianMutationCysteines 49, 50, 83, and 103 to AlaninePromoterCMV IEAvailable sinceSept. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJK526
Plasmid#155387PurposePphlF-TA4 crRNAs, 1x cascade. dCas12a (D917A) + PA4-mVenusDepositorInsertsdCas12a (F. novicida)
PA4-mVenus
PphlF-TA4 in dCas12a oscillator crRNAs
UseCRISPRTagsExpressionBacterialMutationD917A (nuclease-deactivating)PromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST eIF2beta
Plasmid#45900DepositorInserteIF2S2 (Eif2s2 Mouse)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSV HA eIF2beta WT
Plasmid#45898DepositorInsertEif2s2 (Eif2s2 Mouse)
UseTagsHAExpressionMammalianMutationPromoterSV40Available sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL2-MCT2Prom4.2
Plasmid#87347PurposeHuman monocarboxylate transporter 2 (MCT2) promoter 4.2 kbpDepositorInsertMCT2 (SLC16A7 Human)
UseLuciferaseTagsExpressionBacterial and MammalianMutationPromoter4.2 kbp proximal promoterAvailable sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pUC-T7-hTP53-P0~670-FLuc
Plasmid#208640Purposefor in vitro transcription template preparation, insert T7 promoter, TP53 translation initiation site P0 to +670 and F-luc from pGL3-Basic, into pUC19. P0~670 is confusing, BLAST is recommended.DepositorInsertTP53, UTR and partial CDS (TP53 Human)
UseLuciferase; In vitor transcription templateTagsFirefly Luciferase (F-Luc) and T7 promoterExpressionMutationUTR and partial CDS in-frame with F-LucPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-NHERF1 3xSA
Plasmid#195207PurposeMammalian expression vector for GFP tagged NHERF1. Triple PKC site phosphorylation-deficient mutant (S162/339/340A)DepositorInsertNHERF1 (NHERF1 Human)
UseTagsExpressionMammalianMutationS162/339/340APromoterAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ7-DadR/DadS-Pdadh-LacZ
Plasmid#191622PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), Pdadh dopamine dehydroxylase promoter-lacZ with transcriptional regulators DadR/DadSDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ8-CadR-Pcadh-LacZ
Plasmid#191623PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), Pcadh catechin dehydroxylase promoter-lacZ with transcriptional regulator CadRDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only