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Plasmid#67489PurposeTargets HA-eGFP to mitochondrial intermembrane space (IMS)DepositorInsertSMAC (DIABLO Human)
UseTagsHA-eGFPExpressionMammalianMutationaa1-59Promoterattenuated CMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV2-SMAC-HA-eGFP
Plasmid#67488PurposeTargets HA-eGFP to mitochondrial intermembrane space (IMS)DepositorInsertSMAC (DIABLO Human)
UseTagsHA-eGFPExpressionMammalianMutationaa1-59Promoterattenuated CMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-SMAC-HA-eGFP
Plasmid#67491PurposeTargets HA-eGFP to mitochondrial intermembrane space (IMS)DepositorInsertSMAC (DIABLO Human)
UseTagsHA-eGFPExpressionMammalianMutationaa1-59Promoterattenuated CMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-ARF3-V5-His6
Plasmid#67433PurposeMammalian expression of hArf3 with C-term V5 and His6 tagDepositorInsertARF3 (ARF3 Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-ARFRP1-V5-His6
Plasmid#67450PurposeMammalian expression of hArfrp with C-term V5 and His6 tagDepositorInsertARFRP1 (ARFRP1 Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARF5
Plasmid#67456PurposeMammalian expression of hArf5 with stop codon (native expression)DepositorInsertARF5 (ARF5 Human)
UseTagsExpressionMammalianMutationbp 477 T to C; silent mutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARF3-V5-His6
Plasmid#67412PurposeExpression of hArf3 with C-term V5 and His6 tag in E. coliDepositorInsertARF3 (ARF3 Human)
UseTagsHis and V5ExpressionBacterialMutationPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARF4-V5-His6
Plasmid#67413PurposeExpression of hArf4 with C-term V5 and His6 tag in E. coliDepositorInsertARF4 (ARF4 Human)
UseTagsHis and V5ExpressionBacterialMutationPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARF5-V5-His6
Plasmid#67414PurposeExpression of hArf5 with C-term V5 and His6 tag in E. coliDepositorInsertARF5 (ARF5 Human)
UseTagsHis and V5ExpressionBacterialMutationPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDSHA-ARFRP1-Myc
Plasmid#67366PurposeMammalian expression of hArfrp with C-term Myc tagDepositorInsertARFRP1 (ARFRP1 Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARF4-no stop
Plasmid#67311PurposehArf4 without stop codon in Gateway Entry vectorDepositorInsertARF4 (ARF4 Human)
UseGateway entry vectorTagsExpressionBacterialMutationV53A, L123P and M134I mutations were unintended P…PromoterAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA-CBh-eGFP
Plasmid#206885Purposecloning backbone for sgRNA using OptScf2DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterU6Available sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARFRP1-V5-His6
Plasmid#67429PurposeExpression of hArfrp with C-term V5 and His6 tag in E. coliDepositorInsertARFRP1 (ARFRP1 Human)
UseTagsHis and V5ExpressionBacterialMutationPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVNP3.0-PEmax
Plasmid#206883PurposeExpresses FLAG-tagged PEmax fused to Gag through a linker sequenceDepositorInsertPEmax
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterCMVAvailable sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVNP3.0-ABE8e
Plasmid#206882PurposeExpresses FLAG-tagged ABE8e fused to Gag through a linker sequenceDepositorInsertABE8e
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterCMVAvailable sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVNP3.0-PEmaxRH
Plasmid#206884PurposeExpresses FLAG-tagged PEmax (lacking the RNAseH domain) fused to Gag through a linker sequenceDepositorInsertPEMax Delta RNAseH
UseTagsFLAGExpressionMutationPromoterCMVAvailable sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVNP2.0-SpCas9
Plasmid#206880PurposeExpresses FLAG-tagged SpCas9 fused to the N-terminus of Gag/GagPol harbouring an intervening phospholipase C-δ1 pleckstrin homology (PH) domainDepositorInsertSpCas9 (cas9 )
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterCMVAvailable sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
The Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit
Plasmid Kit#1000000210PurposeToolkit contains Cas9-gRNA vectors, integration plasmids, and organellar localization markers as a set of plasmids for Cas9-mediated integration of tagged genes and markers into the yeast genome.DepositorAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGLOW30
Plasmid#172697PurposeC. elegans mScarlet co-injection markerDepositorInsertPmyo-2::mScarlet
UseTagsmScarletExpressionWormMutationPromotermyo-2Available sinceSept. 2, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGLOW31
Plasmid#172698PurposeC. elegans mScarlet co-injection markerDepositorInsertPmyo-3::mScarlet
UseTagsmScarletExpressionWormMutationPromotermyo-3Available sinceAug. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits