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Showing: 941 - 960 of 3847 results

  1. pSLQ8629 pHR-HREYBTATA-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-EFS-Puro-WPRE

    Plasmid
    #140227
    Purpose
    Hypoxia-inducible expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPR. Constitutive EFS-driven puromycin resistance.
    Depositor
    Stanley Qi
    Article
    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2
    Insert
    zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    HRE (Hypoxia inducible)
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140227-plasmid-map-sequence-id-291996

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  4. pSF3-Flag-Cbir-Cdc25C S216A_Myc-Nbir-14-3-3e

    Plasmid
    #90011
    Purpose
    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C S216A and N-terminally tagged NBir[2-256]-14-3-3e
    Depositor
    Julien Béthune
    Article
    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.
    Inserts
    CBirA*-Cdc25C S216A
    NBirA*-14-3-3e
    Use
    Retroviral; Flp/frt
    Tags
    FLAG and Myc
    Expression
    Mammalian
    Mutation
    Ser 216 to Ala
    Promoter
    CMV
    Available since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    90011-plasmid-map-sequence-id-201243

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  5. exon L-firefly

    Plasmid
    #81006
    Purpose
    luciferase based mini-gene to report splicing at exon 25 in Drosophila parayltic
    Depositor
    Richard Baines
    Article
    Lin et al Brain. 2015 Apr;138(Pt 4):891-901. doi:
    Insert
    paralytic (para Fly)
    Use
    Tags
    firefly luciferase
    Expression
    Insect
    Mutation
    spanning exon 24 to exon 26, termination codon in…
    Promoter
    P-AC5 (Actin 5C)
    Available since
    Aug. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    81006-plasmid-map-sequence-id-158526

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  12. exon K-renilla

    Plasmid
    #81007
    Purpose
    luciferase based mini-gene to report splicing at exon 25 in Drosophila parayltic
    Depositor
    Richard Baines
    Article
    Lin et al Brain. 2015 Apr;138(Pt 4):891-901. doi:
    Insert
    paralytic (para Fly)
    Use
    Tags
    Renilla luciferase
    Expression
    Insect
    Mutation
    spanning exon 24 to exon 26, termination codon in…
    Promoter
    P-AC5 (Actin 5C)
    Available since
    Aug. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    81007-plasmid-map-sequence-id-158524

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  14. PB-CAG-dCasRx-RBM25-IRES-BleoR

    Plasmid
    #221002
    Purpose
    Stable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.
    Depositor
    Benjamin Blencowe, Mikko Taipale
    Article
    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e
    Insert
    dCasRx-RBM25-IRES-BleoR
    Use
    CRISPR
    Tags
    NLS and NLS-HA
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    221002-plasmid-map-sequence-id-436162

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  16. (383) pcDNA3.1-HA-nBC2-OGA(544-706)

    Plasmid
    #168100
    Purpose
    HA- tagged BC2 nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA
    Depositor
    Christina Woo
    Article
    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/
    Insert
    HA-nBC2-OGA(544-706)
    Use
    Tags
    HA-tag
    Expression
    Mammalian
    Mutation
    OGA truncated to contain residues 544-706
    Promoter
    T7/CMV
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    168100-plasmid-map-sequence-id-333446

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  17. (271) pcDNA3.1-HA-nEPEA-(EAAAK)3-OGA(544-706)

    Plasmid
    #168102
    Purpose
    HA- tagged EPEA nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA
    Depositor
    Christina Woo
    Article
    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/
    Insert
    HA-nEPEA-(EAAAK)3-OGA(544-706)
    Use
    Tags
    HA-tag
    Expression
    Mammalian
    Mutation
    OGA truncated to contain residues 544-706
    Promoter
    T7/CMV
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    168102-plasmid-map-sequence-id-333448

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Showing: 941 - 960 of 3847 results