Showing: 941 - 960 of 1885 results
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Plasmid#44249PurposeaTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdownDepositorInsertdCas9 (bacteria)
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterpLtetO-1AvailabilityAcademic Institutions and Nonprofits only -
FokI-dCas9
Plasmid#52970PurposeExpresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cellsDepositorInsertFok1 fused to dCas9
UseCRISPRTagsFok1 nuclease (homodimeric wildtype) and NLSExpressionMammalianMutationD10A and H840A in Cas9PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dCas9-T2A-GFP
Plasmid#53191PurposeCo-expresses human optimized S. pyogenes dCas9 and GFPDepositorInserthumanized dead Cas9 T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMammalianMutationD10A and H840APromoterhUbCAvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dCas9 VP64-T2A-GFP
Plasmid#53192PurposeCo-expresses human optimized S. pyogenes dCas9-VP64 and GFPDepositorInserthumanized dead Cas9 VP64 T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMammalianMutationD10A and H840APromoterhUbCAvailabilityAcademic Institutions and Nonprofits only -
pRS315e_pGal-dCas9-SH3
Plasmid#79613PurposeExpresses dCas9-SH3 in yeast cellsDepositorInsertSpCas9
UseTagsSH3 domainExpressionYeastMutationD10A and H840A for nuclease deficient Cas9PromoterpGal1AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dCas9 KRAB-T2A-GFP
Plasmid#67620PurposeCo-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFPDepositorInserthumanized dead Cas9 KRAB T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMutationD10A and H840APromoterAvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/p-bacteria
Plasmid#64325PurposeExpresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPR; Tetracycline-inducibleTags3xFLAGExpressionBacterialMutationD10A, H840APromoterpLtetO-1AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pTEF1p-CYC1t
Plasmid#62190PurposeExpresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPRTags3xFLAG and NLSExpressionYeastMutationhuman codon-optimized, D10A, H840APromoterTEF1 promoterAvailabilityAcademic Institutions and Nonprofits only -
pAN-PTet-dCas9
Plasmid#62244Purposecontrols the expression of S. pyogenes dCas9 from a Tc‐inducible PTet promoter.DepositorInsertdCas9
UseCRISPRTagsExpressionBacterialMutationcatalytically inactive, D10A, H840APromoterPtetAvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP
Plasmid#66707Purposeexpresses a single gRNA and VP64-dCas9-VP64DepositorInsertshU6-gRNA expression cassette
S.Pyogenes VP64-dCas9-VP64
UseLentiviral and Synthetic BiologyTagsVP64ExpressionMutationD10A and H840APromoterhU6 and hUbCAvailabilityAcademic Institutions and Nonprofits only -
pEF_dCas9-VP64
Plasmid#68417PurposeTransient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter.DepositorInsertdCas9
UseCRISPRTags3xFLAG and VP64ExpressionMammalianMutationD10A/H841APromoterhuman EF1[alpha]AvailabilityAcademic Institutions and Nonprofits only -
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pRK5M-ADAM10
Plasmid#31717DepositorInsertADAM10 (ADAM10 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
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PET11a-Af2sir2
Plasmid#8383DepositorUseTagsExpressionBacterialMutationPET11a-Af2Sir2D103NPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMLM3705
Plasmid#47754PurposeExpresses mammalian cell codon-optimized dCas9-VP64DepositorInsertcodon optimized dCas9-VP64
UseCRISPRTags3x FLAG, NLS, and VP64ExpressionMammalianMutationD10A and H840A mutations in Cas9 (catalytically i…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pHAGE TRE dCas9
Plasmid#50915PurposeTet-regulatable dCas9 lentiviral expression vectorDepositorInsertdCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A, H840APromoterTREAvailabilityAcademic Institutions and Nonprofits only -
pHAGE EF1α dCas9-VP64
Plasmid#50918PurposeConstitutive dCas9-VP64 lentiviral expression vectorDepositorInsertdCas9
UseCRISPR and LentiviralTags3xHA and VP64ExpressionMammalianMutationD10A, H840APromoterEF1alphaAvailabilityAcademic Institutions and Nonprofits only -
pHAGE EF1α dCas9-KRAB
Plasmid#50919PurposeConstitutive dCas9-KRAB 2nd generation lentiviral expression vectorDepositorInsertdCas9
UseCRISPR and LentiviralTags3xHA and KRABExpressionMammalianMutationD10A, H840APromoterEF1alphaAvailabilityAcademic Institutions and Nonprofits only
Showing: 941 - 960 of 1885 results