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Plasmid#173889PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-KalTA4; gcry1: BFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT15416-BEAR-mScarlet-target-BFP
Plasmid#162996Purposeplasmid expressing an sgRNA targeting the BEAR-mScarlet plasmid along with a TagBFP markerDepositorInsertsgRNA targeting the BEAR-mScarlet plasmid
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEJS507-pCDest2-noAcr-mTagBFP2-IRES
Plasmid#85748PurposeExpresses mTagBFP2 only; used as a controlDepositorInsertmTagBFP2
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-KalTA4-; gcry1:BFP -1
Plasmid#173890PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-KalTA4; gcry1: BFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BFP-pA_U6-BbsI (pE058)
Plasmid#149352PurposeMammalian expression vector for BFPDepositorInsertBFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(gKAT7-A10)-PGKpuro2ABFP-W
Plasmid#159288PurposeLentiviral vector expressing gRNA targeting KAT7 (gRNA ID. A10)DepositorInsertguide RNA targeting KAT7 (ID. A10) (KAT7 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhuman U6 promoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-NEO-CAG-BFP-pA-GFP-pA
Plasmid#149344PurposeVector for targeting the Replace reporter to the AAVS1 locusDepositorInsertBFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mTagBFP2-T2A-iCreERT2 (PAA)
Plasmid#137859PurposeMonomeric TagBFP2 and iCre-ERT2 recombinase (PAA N-terminus)DepositorInsertmTagBFP2-T2A-iCreERT2
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR_5xUAS-BFP-WPRE_PGK-HygR-bGHpolyA
Plasmid#216665PurposeExpresses Gal4 inducible BFP, with hygromycin resistanceDepositorInsertsBFP
HygromycinR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-hU6-crBACH1-array_EF1a-BFP
Plasmid#224787PurposeBACH1 targeting crRNA array for RfxCas13d expressed from multiple hU6 promoter and reporter BFP protein expressed from EF1a promoterDepositorInsertcrBACH1-1, crBACH1-2, crBACH1-3,
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhU6 and hU6-2xTetOAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-hU6-crCoV-Mutiplex_EF1a-BFP
Plasmid#224788PurposeSARS-COV-2 targeting crRNA array for RfxCas13d expressed from single hU6 promoter and reporter BFP protein expressed from EF1a promoterDepositorInsertcrCoV20, crCoV21, crCoV24
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhU6 and hU6-2xTetOAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-IRES-mTagBFP2-pA (JDW 1361)
Plasmid#224535PurposeA Gateway compatible 3' entry clone containing an IRES-FLAG-NLS-mTagBFP2-pADepositorInsertIRES-FLAG-NLS-mTagBFP2-pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T12 GG2-ER-tagBFP-tUBQ10-GG3
Plasmid#219695PurposeLevel 0 to construct history-dependent targetDepositorInsertGG2-ER-tagBFP-tUBQ10-GG3
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1T15 - T2PsB (mtagBFP2, mCherry, NeonGreen)
Plasmid#219689PurposeLevel 1 preswitched History-dependent target without promoter, switch by Bxb1, output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).DepositorInsertT2PsB (mtagBFP2, mCherry, NeonGreen)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hyg-BFP-P2A-ppViperin
Plasmid#217481PurposeExpression of Blue Fluorescent Protein - P2A - full length protein kinase R from chinook salmonDepositorInsertBFP-P2A-otPKRFL
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Pur-BFP-P2A-ppViperin
Plasmid#217483PurposeExpression of Blue Fluorescent Protein - P2A - short length protein kinase R from chinook salmonDepositorInsertBFP-P2A-otPKRSL
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Zeo-BFP-P2A-ppViperin
Plasmid#217482PurposeExpression of Blue Fluorescent Protein - P2A - medium length protein kinase R from chinook salmonDepositorInsertBFP-P2A-otPKRML
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR Ef1a-dCas9-BFP-KRAB
Plasmid#217304Purposelentiviral vector for dCas9-BFP-KRAB on Ef1alpha promoterDepositorInsertdCas9-tagBFP-KRAB
UseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMutationPromoterEf1alphaAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT2-3X-FLAG-H3.1-K27M-BFP
Plasmid#209439PurposeSB-transposable plasmid expressing a FLAGGED mutant histone H3.1-K27M with BFPDepositorInsertH3.1-K27M
UseTagsExpressionMammalianMutationK27MPromoterAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Zeo-BFP-P2A-otPKR-ML
Plasmid#214365PurposeExpression of the Blue Fluorescent Protein - P2A - medium length protein kinase R from chinook salmonDepositorInsertBFP-P2A-otPKRML
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only