-
Plasmid#119718PurposeExpresses EGFP-tagged human importinα in mammalian cellsDepositorInsertimportinα (KPNA2 Human)
UseTagsEGFPExpressionMammalianMutationcontains amino acids 251-529PromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF2545
Plasmid#144038PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCEBPB (CEBPB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/opto-a1AR-EYFP
Plasmid#20947PurposeMammalian expression of opto-a1AR fused to EYFP for optical control of GPCR signalingDepositorInsertopto-a1AR
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-PDGFRA
Plasmid#23892DepositorInsertPDGFRA (PDGFRA Human)
UseGateway donor vectorTagsExpressionMutationM260IPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
CN1390-rAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA (AAV PHP.eB)
Viral Prep#163505-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from CN1390-rAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA (#163505). In addition to the viral particles, you will also receive purified CN1390-rAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA plasmid DNA. AAV vector for strong and rapid SYFP2 expression in forebrain GABAergic interneurons. The enhancer core sequence has 100% sequence conservation between mouse and human. Developed especially for rapid viral labeling in human and NHP organotypic brain slice culture. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.PromoterminBetaGlobinTagsSYFP2Available sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
HLTV-hTRIM21
Plasmid#104973PurposePurification of His-Lipoyl-hTRIM21 protein from bacteriaDepositorInsertTripartite Motif containing 21 (TRIM21 Human)
UseTagsHis-LipoylExpressionBacterialMutationPromoterT7Available sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe-PDGF/B-zeo
Plasmid#17757DepositorInsertPDGF/B (PDGFB Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
AAV SYN PSAM4 GlyR IRES EGFP
Plasmid#119742PurposeChemogenetics expression plasmidDepositorUseAAVTagsExpressionMammalianMutationL131G, Q139L, Y217FPromotersynapsinAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUAST-pHuji-GINKO2
Plasmid#177117PurposeGenetically encoded green fluorescent potassium ion indicator GINKO2 and red fluorescent pH indicator pHuji fusionDepositorInsertpHuji-GINKO2
UseTagsExpressionInsectMutationPromoterhsp70 promoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYLsgRNA-AtU3b
Plasmid#66198Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAtU3bAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR
Plasmid#60226PurposeExpresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.DepositorInsertssgRNA
Renilla luciferase
Cre recombinase
UseAAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…TagsCre-HA and Rluc-P2AExpressionMammalianMutationPromoterEFS and U6Available sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET30a(+)-PE2
Plasmid#180015PurposeExpressing PE2 protein in E.coli for protein purificationDepositorInsertPE2
UseTags6xHisExpressionBacterialMutationPromoterLac operator-controlled T7 promoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pyEvolvR (enCas9-PolI5M)
Plasmid#158073PurposeExpresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI5M
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMaRSC
Plasmid#89189PurposeThe pMaRS plasmid is the same construct as pMaRSL274G, but contains a T2A-Mcherry sequence appended to the C-terminal of L274GMmMetRS.DepositorInsertFlag-L274G-T2A-mCherry (Mars1 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-HA-hTAF1
Plasmid#17997DepositorInserthTAF1 (TAF1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
TFORF0589
Plasmid#142653PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertFOXO1 (FOXO1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL wt-pBabe-puro
Plasmid#19234DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationwtPromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCDNA 3 5' cMyc SMC1 wt
Plasmid#32363DepositorInsertSMC1A (SMC1A Human)
UseTagsc-MycExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGL3basic luciferase and renilla_polyA
Plasmid#128046PurposepGL3 basic luciferase reporter with co-expression of renilla luciferaseDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmVen-DHX9
Plasmid#211409Purposemammalian expression of human DHX9 N-terminally fused to mVenusDepositorInsertDHX9 (DHX9 Human)
UseTagsmVenusExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only