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Plasmid#148757PurposeMammalian Expression of HsNot4_377-428DepositorInsertHsNot4_377-428 (CNOT4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pShuttle.Cre
Plasmid#192930PurposeGateway compatible vector with BsaI cloning site and Cre expressionDepositorTypeEmpty backboneUseGateway-compatible cloning vectorTagsExpressionMutationPromoterCMVAvailable sinceDec. 9, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pT7-MS2-HA-C1-HsGIGYF1_1-671_AI
Plasmid#148958PurposeMammalian Expression of HsGIGYF1_1-671DepositorInsertHsGIGYF1_1-671 (GIGYF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsGIGYF1_AI
Plasmid#148961PurposeMammalian Expression of HsGIGYF1DepositorInsertHsGIGYF1 (GIGYF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF1_1-671_AI
Plasmid#148943PurposeMammalian Expression of HsGIGYF1_1-671DepositorInsertHsGIGYF1_1-671 (GIGYF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF1-F306AF312A_AI
Plasmid#148945PurposeMammalian Expression of HsGIGYF1-F306AF312ADepositorInsertHsGIGYF1-F306AF312A (GIGYF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF1-W294A_AI
Plasmid#148946PurposeMammalian Expression of HsGIGYF1-W294ADepositorInsertHsGIGYF1-W294A (GIGYF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF1-W294AF306AF312A_AI
Plasmid#148947PurposeMammalian Expression of HsGIGYF1-W294AF306AF312ADepositorInsertHsGIGYF1-W294AF306AF312A (GIGYF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-MBP-GGSG-DmBam_13-36_AG
Plasmid#148841PurposeMammalian Expression of DmBam_13-36DepositorInsertDmBam_13-36 (bam Fly)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-DmRoquin-del725-755-del790-812_AE
Plasmid#148634PurposeMammalian Expression of DmRoquin-del725-755-del790-812DepositorInsertDmRoquin-del725-755-del790-812 (roq Fly)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUBI-RZ-Cas12j2
Plasmid#189781PurposeCas12j2 (CasΦ) Gateway gRNA entry plasmid using Zea mays Ubi promoter and HH, HDV ribozyme processingDepositorInsertgRNA cloning site
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-Ark-L2
Plasmid#186362PurposeEntry clone with ORF encoding Ciona robusta Aurora kinase A flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.DepositorInsertCiona robusta Aurora kinase A
UseExpression of a fluorescent centrosome markerTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-HistoneH2B-L2
Plasmid#186357PurposeEntry clone with ORF encoding histone H2B flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.DepositorInsertHistone H2B (H2BC11 Human)
UseExpression of a tagged nuclear markerTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTln1-F2-P229S-pET151
Plasmid#177871PurposeExpresses mouse Talin 1 F2-P229S domain in bacterial cellsDepositorInsertmouse Talin 1 F2 P229S mutant (Tln1 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationchanged Proline 229 to Serine (P229S)PromoterT7Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTln1-F2-P229L-pET151
Plasmid#177876PurposeExpresses mouse Talin 1 F2-P229L domain in bacterial cellsDepositorInsertmouse Talin 1 F2 P229L mutant (Tln1 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationchanged Proline 229 to Leucine (P229L)PromoterT7Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-MBP-ATG9
Plasmid#188076PurposeRecombinant overexpression of human ATG9 in mammalian cellsDepositorInsertATG9 (ATG9A Human)
UseTagsMBP tagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2
Plasmid#186413PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined NLSLacZ under control of an AttB3/B5 recombined reg. sequence.DepositorInsertlacZ (lacZ E. coli)
UseGateway destination vectorTagsNuclear localization signalExpressionMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG8H-sumo-EWS_91-199
Plasmid#180465PurposeExpresses the construct His8-sumo-EWS_91-199DepositorInsertEWSR1 (EWSR1 Human)
UseTagsHis8 and SUMOExpressionBacterialMutationdeleted 1-90, 200-656PromoterT7Available sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FluoSTEP-AKAR (C/Y)
Plasmid#181862PurposeFluoSTEP-AKAR using split CFP and cpVenus(E172) as the FRET donor and acceptor. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-AKAR (C-Y)
UseTagsCFP(1-10) and cpVenus(E127)ExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-GFP11-RhoA
Plasmid#181860PurposePart of FluoSTEP-RhoA biosensor; co-express with cpPKN-mRuby2-GFP1-10 (Addgene #181859).DepositorInsertGFP11-RhoA (RHOA Human)
UseTagsGFP11ExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only