Addgene

We narrowed to 53,161 results for: %s

Showing: 9501 - 9520 of 53161 results

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pENTR-2B-Tbx5

Plasmid

#98619

Purpose

Gateway entry vector TBX5

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

TBX5 (Tbx5 Mouse)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pTBRII / CEP-Zeo / Hygro

Plasmid

#16622

Depositor

Bert Vogelstein

Article

Zhou et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Insert

TGF beta type II receptor (TGFBR2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
pCMV-ACBEmax

Plasmid

#139106

Purpose

Mammalian ACBEmax expression vector (pSI-632)

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pInducer10-UBC9-sh4

Plasmid

#168988

Purpose

Expresses RFP cDNA and UBC9 shRNA 4 in mammalian cells

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Ubiquitin Conjugating Enzyme 9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ubc promoter

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCALNL-mutKir2.1

Plasmid

#125579

Purpose

Cre-dependent expression of a non-conducting mutant of Kir2.1 (FLAG-tagged) in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

Kir2.1 (Kcnj2 Mouse)

Use

Cre/Lox

Tags

FLAG

Expression

Mammalian

Mutation

A non-conducting mutant (GYG -> AAA).

Promoter

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBMN HA-ATG13

Plasmid

#186223

Purpose

For expression of ATG13 tagged with HA

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

ATG13 (ATG13 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pET-15b-LanCL1

Plasmid

#154189

Purpose

To express LanCL1 in bacterial cells

Depositor

Wilfred van der Donk

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

LanCL1

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold-Mito-N-7

Plasmid

#158007

Purpose

Visualization of mitochondria in mammalian cells using mGold

Depositor

Francois St-Pierre

Article

Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

Insert

Mito (COX8A Human)

Use

Tags

mGold

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pv9_CBX7_2xChromo-13XL-BASU

Plasmid

#179413

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7.2xChromo-13XL-BASU

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX7.2xChromo-13XL-BASU

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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MEIS1A-MIY

Plasmid

#21013

Depositor

Corey Largman

Article

Fischbach et al Blood. 2005 Feb 15. 105(4):1456-66.

Insert

MEIS1A (Meis1 Mouse)

Use

Retroviral

Tags

IRES and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pRSpuro shID3#1

Plasmid

#19163

Depositor

Joan Massague

Article

Gupta et al Proc Natl Acad Sci U S A. 2007 Dec 4. 10

Insert

small hairpin RNA against inhibitor of DNA binding 3 (ID3 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 16, 2008

Availability

Academic Institutions and Nonprofits only
pX330A-Yap1-E1-#1

Plasmid

#171517

Purpose

deletion of a genomic locus in Yap1 gene

Depositor

Hans Schöler

Article

Kagiwada et al Development. 2021 Oct 15;148(20). pii: 2

Insert

Yap1 (Yap1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pX330A-Yap1-E2-#1

Plasmid

#171518

Purpose

deletion of a genomic locus in Yap1 gene

Depositor

Hans Schöler

Article

Kagiwada et al Development. 2021 Oct 15;148(20). pii: 2

Insert

Yap1 (Yap1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGST2-GST-TEV-NDP52

Plasmid

#187828

Purpose

Plasmid for the expression and purification of GST-TEV-NDP52 in E. coli Rosetta pLysS. Internal reference: SMC1586

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NDP52 (CALCOCO2 Human)

Use

Tags

GST-TEV

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-RSV-GFP-U6-Rosa26 gRNA (SpyCas9 scaffold)

Plasmid

#120296

Purpose

AAV vector; encodes GFP as well as a U6-driven Rosa26-targeting gRNA (SpyCas9 scaffold)

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

Rosa26 gRNA (SpCas9 scaffold)

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pGMC00034 (aka pMC0235)

Plasmid

#210751

Purpose

Non-targeting guide RNA

Depositor

Raj Chari

Article

Ohara et al Cell Rep. 2023 Nov 18;42(12):113434. doi

Insert

Non-targeting guide RNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pv6_dPC_Chromo_TAF3_Phd

Plasmid

#179403

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of dPC_Chromo - TAF3_Phd

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

dPC_Chromo - TAF3_Phd

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA pDEST40 Cas9-HMGB1

Plasmid

#183195

Purpose

Expression clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

HMGB1

Use

CRISPR

Tags

Cas9WT

Expression

Mammalian

Mutation

n/a

Promoter

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCIG AKT3 K177M

Plasmid

#73051

Purpose

Human AKT3 K177M

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

AKT3 K177M (AKT3 Human)

Use

Tags

Expression

Mammalian

Mutation

K177M

Promoter

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GFP-MTn-KDEL

Plasmid

#154348

Purpose

Mammalian expression of MTn

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

MTn

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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