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Showing: 9481 - 9500 of 34890 results

  3. pISV-DsRed1-nopP

    Plasmid
    #171025
    Purpose
    Agrobacterium rhizogenes-mediated hairy root transformation of Lotus japonicus with nopP, a type 3 effector gene of Sinorhizobium sp. NGR234.
    Depositor
    Christian Staehelin
    Insert
    nopP
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    2 X EN 35S
    Available since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    171025-plasmid-map-sequence-id-337715

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  4. pB2H6

    Plasmid
    #163835
    Purpose
    Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and SHP1
    Depositor
    Jerome Fox
    Insert
    SpecR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pro1;ProD;pLacZ-Opt
    Available since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    163835-plasmid-map-sequence-id-337603

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  5. pGBK CUP1 Empty

    Plasmid
    #169710
    Purpose
    Yeast two-hybrid vector using a copper inducible CUP1 promoter
    Depositor
    Bruce Hamilton
    Article
    Hernandez et al bioRxiv
    Type
    Empty backbone
    Use
    Tags
    GAL4 DNA Binding Domain and Myc
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    CUP1
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169710-plasmid-map-sequence-id-335105

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  6. pANY4-pL18-ccdB

    Plasmid
    #164898
    Purpose
    Two valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pL18-ccdB.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    164898-plasmid-map-sequence-id-327989

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  7. HCP1

    Plasmid
    #166103
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination.
    Depositor
    Matthias Heinemann
    Insert
    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166103-plasmid-map-sequence-id-330378

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  8. HCP2

    Plasmid
    #166104
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination
    Depositor
    Matthias Heinemann
    Insert
    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166104-plasmid-map-sequence-id-330375

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  9. HCP3

    Plasmid
    #166105
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination
    Depositor
    Matthias Heinemann
    Insert
    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166105-plasmid-map-sequence-id-330319

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  10. J-CDAN1A-5

    Plasmid
    #166522
    Purpose
    scFv of a human scaffold targeting Codanin-1. Antigen coverage aa 840-1202 of 1227
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166522-plasmid-map-sequence-id-328268

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  11. Z-OPTN-10

    Plasmid
    #166562
    Purpose
    scFv of a human scaffold targeting Optineurin. Antigen coverage aa 1-510 of 577
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    March 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    166562-plasmid-map-sequence-id-327812

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  13. 6V41

    Plasmid
    #162272
    Purpose
    Baculovirus expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    CDY1 (CDY1 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    162272-plasmid-map-sequence-id-319838

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  14. 6WAU

    Plasmid
    #162269
    Purpose
    Bacterial Expression
    Depositor
    Cheryl Arrowsmith
    Insert
    PHF19 (PHF19 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    162269-plasmid-map-sequence-id-319835

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  15. 6WAJ

    Plasmid
    #162267
    Purpose
    Bacterial Expression
    Depositor
    Cheryl Arrowsmith
    Insert
    NLE1 (NLE1 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    162267-plasmid-map-sequence-id-319833

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  16. pSMPUW_8.3_M4_CAMKIIA-gateway-V5-SV40-Zeo

    Plasmid
    #128463
    Purpose
    lentiviral destination vector with CAMKIIA promoter, C-terminal V5 tag, SV40 promoter, and Zeo Resistance gene
    Depositor
    Lindy Barrett
    Insert
    CAMKIIA promoter, C-terminal V5 tag, SV40 promoter, and Zeocin Resistance gene
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    128463-plasmid-map-sequence-id-270529

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  17. pJR288

    Plasmid
    #78550
    Purpose
    For expressing small noncoding RNAs from U6 promoter. One step cloning of oiigonucleotide pairs containing CACC and AAAA overhangs. Ef1a driven mCherry visible marker.
    Depositor
    John Rossi
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6, EF1a
    Available since
    Jan. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    78550-plasmid-map-sequence-id-191096

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  18. phSOD1-L126X-N’His

    Plasmid
    #117707
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    SOD1 (SOD1 Human)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    L126X
    Promoter
    T7
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117707-plasmid-map-sequence-id-227541

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  19. pmSod1-Q42L-N’His

    Plasmid
    #117708
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    Sod1 (Sod1 Mouse)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Q42L
    Promoter
    T7
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117708-plasmid-map-sequence-id-227535

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  20. pmSod1-Q123A-N’His

    Plasmid
    #117709
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    Sod1 (Sod1 Mouse)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Q123A
    Promoter
    T7
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117709-plasmid-map-sequence-id-227504

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Showing: 9481 - 9500 of 34890 results