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Plasmid#65289Purposesynchronize trafficking of Ecadherin from the ER (RUSH system)DepositorInsertEcadherin
UseTagsIL-2 Signal Sequence and Streptavidin Binding Pro…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
NM1-5S-tRNA-SgH
Plasmid#128178PurposeExpresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoterDepositorInsertgRNA cloning cassette
UseCRISPRTagsExpressionYeastMutationPromoter5S rRNA-tRNA(Arg)Available sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_Ado1.0med
Plasmid#153286PurposeExpress the medium-affinity adenosine sensor GRAB_Ado1.0med in neuronsDepositorInsertAdenosine sensor GRAB_Ado1.0med (medium affinity)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJuly 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK437 (mAID-mCherry-P2A-BSD)
Plasmid#168232PurposeC-terminal tagging with mAID-mCherryDepositorInsertmAID-mCherry-P2A-BSD
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
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KARS_pNIC-Bio3
Plasmid#153051PurposeExpression construct for producing Avi tagged Lysyl-tRNA synthetaseDepositorInsertKARS (KARS1 Human)
UseTagsAvi and His6-TEVExpressionBacterialMutationPromoterT7Available sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-crRNA
Plasmid#213753PurposeFor circular RNA-mediated prime editor using LbCas12a-crRNA in HEK293T cellsDepositorInsertLbCas12a-crRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJR101
Plasmid#187241PurposeLentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-GFP marker expressionDepositorInsertLentiviral sgRNA vector for Perturb-seq with mU6 promoter
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
YFP-CL1
Plasmid#11950DepositorInsertYFP-CL1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
enOgeuIscB with ωRNA-v2
Plasmid#220939PurposeCMV-driven expression of enOgeuIscB and human U6-driven expression of ωRNA-v2 scaffold with BsmBI for guide cloingDepositorInsertsCMV-SV40 NLS-enOgeuIscB_nucleoplasmin NLS
OgeuIscB-ωRNA-v2 scaffold with BsmBI for guide cloning
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-v2 Puro KIAA0141/DELE1_sg1
Plasmid#218524PurposesgRNA targeting human KIAA0141/DELE1DepositorInsertDELE1 (DELE1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mCherry-FKBP-GEF(ITSN1)
Plasmid#222631PurposeCdc42 activation using rapamycinDepositorInsertmCherry-FKBP-GEF(ITSN1) (ITSN1 Human)
UseTagsmCherry-FKBPExpressionMammalianMutationPromoterCAGAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBL7056
Plasmid#167225Purposeorthogonal T7 RNAP sfGFP reporterDepositorInsertpAKSIRV-sfGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pUC18T-mini-Tn7T-hph
Plasmid#218366PurposeEmpty backbone for Tn7-site insertionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-hph-Para
Plasmid#218363PurposeEmpty backbone for Tn7-site insertion with araC-ParaBAD expression systemDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMApra-Ptet2
Plasmid#218361PurposeEmpty backbone with tetR-PtetA expression system with two tetR operator sitesDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-NUP37 neo
Plasmid#220361PurposeLentiviral expression vector for an inducible 3xFLAG-tagged NUP37DepositorInsertNUP37 (NUP37 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-v2 Puro KIAA0141/DELE1_sg2
Plasmid#218525PurposesgRNA targeting human KIAA0141/DELE1DepositorInsertDELE1 (DELE1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMApra-Plac-SO
Plasmid#218358PurposeEmpty vector with lacIq-Plac expression systemDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only