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Plasmid#48089PurposeBacterial expression of GST tagged AT-Hook2-R270XDepositorInsertMethyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
UseTagsGSTExpressionBacterialMutationdeleted the N-terminal 256 codons, expresses AT-H…PromoterAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMX-Gal4-hMeCP2-G273X-R111G
Plasmid#48087PurposeMammalian expression of Gal4 tagged MeCP2-G273X-R111GDepositorInsertMethyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
UseTagsGal4ExpressionMammalianMutationR111G mutant MeCP2, expresses AT-hook G273XPromoterAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
p2706-pHAGE-EF1aL-YAP5SA-UBC-GFP
Plasmid#216475Purposelentiviral dual expression of YAP5SA and eGFPDepositorInsertsYAP5SA
eGFP
UseLentiviralTagsExpressionMutationS61A, S109A, S127A, S164A, S381APromoterEF1aL and UBCAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2702-AGER-gRNA1
Plasmid#216471Purposeto express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start siteDepositorInsertsgRNA targeting human AGER locus
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2703-AGER-gRNA2
Plasmid#216472Purposeto express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start siteDepositorInsertsgRNA targeting human AGER locus
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2704-AGER-gRNA3
Plasmid#216473Purposeto express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start siteDepositorInsertsgRNA targeting human AGER locus
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2708-pHAGE2-Ef1aL-dsRed-UBC-tagBFP-loxP
Plasmid#216477Purposelentiviral dual expression of dsRed and tagBFP with loxP site for Cre excisionDepositorInsertsdsRed
tagBFP
UseCre/Lox and LentiviralTagsExpressionMutationPromoterEF1aL and UBCAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PB2-B/BRISBANE/60/2008
Plasmid#187917PurposeExpresses PB2 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cellsDepositorInsertpolymerase basic protein 2
UseTagsUNTAGGEDExpressionMutationPromoterCMV PromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1(Hygro) myc-hPolQ-Flag
Plasmid#73132PurposeTo express in mammalian cells human PolQDepositorInsertPolymerase theta (POLQ Human)
UseTagsFlag and mycExpressionMammalianMutationPromotercmv promoterAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
alpha7nACHR_Nacho_GCAMP7s
Plasmid#182940Purposealpha7 nACHR expression in mammalian cell lines with calcium reporterDepositorInsertNACH7 (CHRNA7 Human)
UseTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
FLAG-FullLength-TET1-WT(21)
Plasmid#124396PurposeFull-length TET1 used to PiggyBAC rescue the function of TET1/2 DKO cellsDepositorInsertFLAG-FullLength-TET1 (TET1 Human)
UseTagsFlagExpressionMammalianMutationfull length protein is 2136 aaPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)::NL3F10H
Plasmid#141290PurposeNLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertNLUC3F10H
UseLuciferaseTagsFlag (x3) and His (x10)ExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGG-GFP-PM-TurboID
Plasmid#209402PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a plasma menbrane targeting peptide in plantaDepositorInsertcTP-3XHA-TurboID
UseTagsGFPExpressionPlantMutationPromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXE1
Plasmid#153120PurposeExpression of Flag tagged human FOXE1 in mammalian cellsDepositorInserthuman FOXE1 (FOXE1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CAG-NCreIntN-T2A-mTagBFP2
Plasmid#160500PurposeExpresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applicationsDepositorInsertNCreIntN
UseCre/Lox and LentiviralTagsmTagBFP2ExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CAG-IntCCCre-T2A-CopGFP
Plasmid#160501PurposeExpresses IntCCCre in mammalian cells in split intein-mediated split-Cre recombinase applicationsDepositorInsertIntCCCre
UseCre/Lox and LentiviralTagsCopGFPExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNos-PE2-attB
Plasmid#149549PurposeExpression of PE2 enzyme under control of the germ cell specific nanos promoter. Can be used to generate transgenic flies with vermillion+ selection.DepositorInsertPE2
UseCRISPRTagsExpressionInsectMutationPromoternanos (nos)Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CAG-NCreIntN-EF1a-mTagBFP2
Plasmid#160507PurposeExpresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applicationsDepositorInsertNCreIntN
UseCre/Lox and LentiviralTagsmTagBFP2ExpressionMammalianMutationPromoterCAGAvailable sinceJan. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Caspase-3(D3A,V266E)
Plasmid#90091PurposeExpresses caspase D3A and V266E mutant in bacterial cellsDepositorInsertCaspase 3 (CASP3 Human)
UseTagsHis tagExpressionBacterialMutationD175A D28A D9A V266EPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_ygiF-CHAD
Plasmid#127793PurposeExpresses the CHAD domain of ygiF in E. coli cellsDepositorInsertygiF-CHAD (ygiF Escherichia coli)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationN-terminal TTM domain of ygiF (residues 201-433)PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only