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Plasmid#60324PurposeHNF4A P2 promoter cloned into the pGL2-basic backbone.DepositorInsertNM_000457.4 (HNF4A Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-ccdB
Plasmid#14148DepositorTypeEmpty backboneUseGateway destinationTagsExpressionYeastMutationPromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTRKH3-slpGFP
Plasmid#27168DepositorInsertsurface (S)-layer protein promoter region fused with modified green fluorescent protein 5
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP7f-WPRE (AAV PHP.eB)
Viral Prep#104488-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pGP-AAV-syn-jGCaMP7f-WPRE (#104488). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7f-WPRE plasmid DNA. Synapsin-driven GCaMP7f calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R777-E283 Hs.ROCK1
Plasmid#70567PurposeGateway ORF clone of human ROCK1 [NM_005406.2] with stop codon (for native or N-terminal fusions)DepositorInsertROCK1 (ROCK1 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEnt L1L3 EF1a-tTA-2
Plasmid#26803DepositorInsertElongation Factor 1 alpha promoter and tTA transgene
UseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBOB-Lck-tdTomato
Plasmid#118739PurposeTo express membrane targeted tdTomatoDepositorInsertLck domain (LCK Human)
UseLentiviralTagstdTomatoExpressionMutationH8N (see comments)PromoterCMVAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
FUW-hTie1-FLAG
Plasmid#119233PurposePlasmid encodes FLAG-tagged full-length human Tie1 gene cloned in lentiviral vector.DepositorInsertTIE1 (TIE1 Human)
UseLentiviralTagsFLAGExpressionMutationPromoterP hUbCAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-miR-34a
Plasmid#63932PurposeMSCV vector for overexpressing mouse miR-34DepositorInsertmicroRNA 34a (Mir34a Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
NYESO alpha into TRAC HDRT Source (pTR 223)
Plasmid#112023PurposeDNA sequence source for amplifying an HDR template to replace endogenous human TCR-alpha with 1G4 NYESO TCR-alphaDepositorInsertNYESO alpha into TRAC HDRT
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-TREX1
Plasmid#27218DepositorInsertTREX1 (TREX1 Human)
UseTagsFLAGExpressionMammalianMutationn/aPromoterAvailable sinceJan. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-2xrGBD
Plasmid#176109PurposeVisualization of active RhoDepositorInsertrhotekin (Rtkn Mouse)
UseTagsExpressionMammalianMutationPromoterCMVdelAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag hHMGB1
Plasmid#31609DepositorInsertHMGB1 (HMGB1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pQE30-HyPer7
Plasmid#141071PurposeBacterial expression of ultrasensitive hydrogen peroxide indicator HyPer7 for optical imagingDepositorInsertHyPer7
UseTagsHisx6ExpressionBacterialMutationPromoterT5Available sinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)
Plasmid#139996PurposeCMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC-Luc
Plasmid#33154DepositorTypeEmpty backboneUseLuciferaseTagsLuciferaseExpressionInsectMutationPromoterActin 5CAvailable sinceDec. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-ATBF1
Plasmid#40927DepositorInsertATBF1 (ZFHX3 Human)
UseTagsFLAGExpressionMammalianMutationSee depositor comments below.PromoterCMVAvailable sinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HA-NLGN3 R451C
Plasmid#59319PurposeExpression plasmid of HA-tagged human Neuroligin 3 harboring an R451C mutationDepositorInsertNLGN3 R451C (NLGN3 Human)
UseTagsHAExpressionMammalianMutationArginine 451 to Cysteine (R451C mutation)PromoterCAGAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV_TRET_hNgn2_UBC_Puro
Plasmid#61474Purpose3rd generation lentiviral vector; TetON promoter driving human Ngn2; constitutive expression of Puromycin selection markerDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-1x
Plasmid#172281PurposeExpressing EGFP mRNA fused with 1 tandem repeat of a 50-base sequenceDepositorInsertEGFP-N1-1x
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only