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Plasmid#110085PurposeReplicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only
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pEY79
Plasmid#191074Purposeflp-12 fluorescent neural reporter driving nuclear T-Sapphire expression (refer to NeuroPAL paper for expression)DepositorInsertflp-12 (flp-12 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY62
Plasmid#191020Purposeacr-5 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertacr-5 (acr-5 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG1_zeo
Plasmid#184910PurposeTemplate to generate via PCR a single gRNA for expression in S. cerevisiaeDepositorInsertgRNA scaffold
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-Sec61B
Plasmid#120244PurposeExpresses V5-tagged Sec61B in lentiviral vectorDepositorInsertSec61B (Sec61b Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMGIB-3xFLAG-reptin
Plasmid#53609PurposeRetroviral expression of human reptinDepositorInsertreptin (RUVBL2 Human)
UseRetroviralTags3xFLAGExpressionMammalianMutationCDS mutated to confer resistance to shRNA. All mu…PromoterLTRAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046758
Plasmid#145670PurposeBacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;HAExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-Barkor (FL)
Plasmid#24394DepositorInsertBarkor (ATG14 Human)
UseTagsHis and MycExpressionMammalianMutationPromoterAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-FBXO31
Plasmid#34815DepositorInsertF-box protein 31 (FBXO31 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR MCS L3-L2
Plasmid#62088PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseMule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-FH-eGFP
Plasmid#157734Purposemammalian expression of N-terminally Flag-His8 tagged eGFP under control of a tetracycline-inducible promoterDepositorInsertFH-eGFP
UseTagsFlag-His8ExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
mcherry Beta 1
Plasmid#36111DepositorInsertG protein beta subunit B1 (GNB1 Bovine)
UseTagsmcherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSVE Grb2 myc 49L
Plasmid#26085DepositorInsertGrb2 P49L (GRB2 Human)
UseTagsmycExpressionMammalianMutationP49LPromoterAvailable sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMTP130 (pTA106_TnsABC(Tn6900))
Plasmid#164261PurposeVector expressing the TnsA, TnsB, and TnsC transposition proteins from the Tn6900 derivative element with a lactose promoter.DepositorInserttnsA, tnsB, and tnsC from Tn6900 derivative from A. salmonicida S44 pS44-1
UseTagsExpressionBacterialMutation(tnsA) Changed Alanine 125 to Aspartic acidPromoterLactoseAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGST-TEV-yVms1(1-417,delta(38-69))
Plasmid#128739PurposeBacterial overexpression and crystallizationDepositorInsertVms1 (VCP/Cdc48-associated mitochondria stress-responsive 1) (VMS1 Budding Yeast)
UseTagsGSTExpressionBacterialMutationresidues 1-417 with deletion of 38-69PromoterT7Available sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
MAC-SARS-CoV-2 NSP2
Plasmid#158353PurposeMAC-tagged gene expressionDepositorInsertSARS-CoV-2 NSP2 (ORF1ab )
UseTagsMAC-tagExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC-SARS-CoV-2 Orf10
Plasmid#158380PurposeMAC-tagged gene expressionDepositorInsertSARS-CoV-2 Orf10 (ORF10 )
UseTagsMAC-tagExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221_SLC22A3
Plasmid#132024PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC22A3 (SLC22A3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC-SARS-CoV-2 NSP12
Plasmid#158363PurposeMAC-tagged gene expressionDepositorInsertSARS-CoV-2 NSP12 (ORF1ab )
UseTagsMAC-tagExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQTEV-UFD1L
Plasmid#31560DepositorInsertubiquitin fusion degradation 1-like (UFD1 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only