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Plasmid#200907PurposeExpresses human codon-optimized E. coli ClpX from a CMV promoter.DepositorInsertEcoClpX
UseTagsNLSExpressionMammalianMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-SI-121
Plasmid#131126PurposeA-to-G base editing reporterDepositorInsertmutant EGFP
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 flag p53
Plasmid#10838DepositorInsertp53 (TP53 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
C1-MPAct-mRuby3
Plasmid#155221PurposeMonitor changes in the density of membrane-proximal F-actin (MPA)DepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
UseTagsmRuby3ExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-14 (LA-14)
Plasmid#158750PurposeLentiviral plasmid for expression of shorter LifeAct variant (up till 14 amino acids) fused with carboxy terminus eGFP in mammalian cells.DepositorInsertLifeAct-14
UseLentiviralTagseGFPExpressionMammalianMutationDeletion mutation for last three amino acids from…PromoterCMV immediate earlyAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLuc
Plasmid#170575PurposeExpress firefly luciferase. Used in MLV-based SARS-CoV-2 pseudovirus assay.DepositorInsertLuc
UseLuciferase and RetroviralTagsNAExpressionMammalianMutationPromoterCMVAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET24b-APEX2
Plasmid#111702PurposeFor IPTG-inducible expression of APEX2 in E. coliDepositorInsertAPEX2
UseTags6xHis and V5ExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSII424
Plasmid#35466DepositorTypeEmpty backboneUseYeast 2 micron vectorTagsExpressionMutationPromoterNoneAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-mSA-EGFP-TM
Plasmid#39863DepositorInsertmonomeric streptavidin, EGFP, TM helix
UseTagsEGFP, FLAG, and HAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
IBMc396
Plasmid#161954PurposeDemonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to acVHHDepositorInsertpSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Human SERCA2a (pcDNA3.1+)
Plasmid#75187PurposeMammalian expression of ATP2A2DepositorInsertATP2A2 (ATP2A2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG_smFP V5
Plasmid#59758Purposeencodes multiple copies of an epitope tag V5DepositorInsertsmFP _V5
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
MoFGFR3b-SV40PA
Plasmid#86493Purposemamalian cell expression of FGFR3bDepositorInsertFgfrR3 IIIb 12F5 (Fgfr3 Mouse)
UseTagsExpressionMammalianMutationPromoterMoloney murine leukemia virus LTRAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 ZoAl N-1F
Plasmid#203763PurposeFor expression of N-flag tagged ZoAl R2 protein in mammalian cellsDepositorInsertR2 retroelement protein
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mCherry-MHC-IIA
Plasmid#35687DepositorInsertMHC IIA (MYH9 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pC.HA Vav1
Plasmid#14553DepositorInsertVav1 (VAV1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hsp27 wt FL
Plasmid#17444DepositorInsertsmall heat shock protein27 (HSPB1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
Myr.PKCalpha.FLAG
Plasmid#10807DepositorInsertPKC alpha (PRKCA Bovine)
UseTagsFLAG and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_nMagHigh1-EGFP-CAAX
Plasmid#67300PurposeBlue light dependent photoswitching systemDepositorInsertnMagHigh1-EGFP-CAAX
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF1alpha P402A/P564A-pBabe-puro
Plasmid#19005DepositorInsertHypoxia inducible factor 1 alpha (HIF1A Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 402 to Alanin…PromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only