Addgene

We narrowed to 1,098 results for: biotin

Showing: 921 - 940 of 1098 results

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D92_Ld_360170

Plasmid

#135905

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein, conserved

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-CSNK2A2_IDG-K

Plasmid

#189870

Purpose

Gateway destination clone of CSNK2A2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

CSNK2A2 (CSNK2A2 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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p667-UBC-COQ8A-V5-miniTurbo_IDG-K

Plasmid

#189885

Purpose

Gateway destination clone of COQ8A (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

COQ8A (COQ8A Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-DCLK3_IDG-K

Plasmid

#189891

Purpose

Gateway destination clone of DCLK3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

DCLK3 (DCLK3 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-LMTK2_IDG-K

Plasmid

#170580

Purpose

Gateway destination clone of LMTK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-LMTK2 (LMTK2 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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p667-UBC-HIPK3-V5-miniTurbo_IDG-K

Plasmid

#158191

Purpose

Gateway destination clone of HIPK3 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

HIPK3-V5-miniTurbo (HIPK3 Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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D38_Ld_341960

Plasmid

#135852

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

thioredoxin, putative

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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D32_Ld_354780

Plasmid

#135848

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

Hsp70 protein, putative

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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D63_Ld_100680

Plasmid

#135876

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

flagellar glycoprotein-like protein

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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D72_Ld_231020

Plasmid

#135885

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein, unknown function

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
D57_Ld_190640

Plasmid

#135870

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

extracellular receptor, putative

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
D30_Ld_260440

Plasmid

#135846

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein, conserved

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
D26_Ld_200260

Plasmid

#135842

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

transmembrane protein, putative

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
D28_Ld_241170

Plasmid

#135844

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein, conserved

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
D29_Ld_251870

Plasmid

#135845

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein, conserved

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
D37_Ld_361830

Plasmid

#135851

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein, conserved

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
D39_Ld_360680

Plasmid

#135853

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein, conserved

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
D41_Ld_180450

Plasmid

#135855

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

serine carboxypeptidase (CBP1), putative

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
D43_Ld_300860

Plasmid

#135857

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein, conserved

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
D58_Ld_280280

Plasmid

#135871

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

carbonic anhydrase-like protein

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge