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Plasmid#89762PurposeQuantitative measurement of CArG response element activity.DepositorInsert6X CArG-RE
UseLentiviralTagsCMV-ZsGreenExpressionMammalianMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-Dynamin 2 K44A
Plasmid#22301DepositorInsertDynamin 2 K44A (Dnm2 Rat)
UseTagsGFPExpressionBacterial and MammalianMutationK44APromoterAvailable sinceDec. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 E-cadherin shRNA/Ecad-tdTomato
Plasmid#101278PurposeLentiviral expression of shRNA targeting Ecad and Ecad-tdtomatoDepositorInsertE-cadherin
UseLentiviralTagstdtomatoExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-H-Ras_V12
Plasmid#39504DepositorInsertHRAS V12 (HRAS Human)
UseTagsExpressionMammalianMutationG12VPromoterCMVAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pCD4-TagRFP-T (RHPMP151)
Plasmid#119238PurposeExpresses CD4 fused to TagRFP-TDepositorInsertCD4 (CD4 Human)
UseTagstagRFP-TExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
cpGFP-Adhiron_flex
Plasmid#165044PurposeFlexible linker control for POLArISact expression in mammalian cellsDepositorInsertcpGFP-Adhiron (connected with a flexible linker)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB80-hsKIF5B(1-560)-L-GFP
Plasmid#193716PurposeExpression of GFP tagged human kinesin-1 (KIF5B aa1-560)DepositorInserthsKIF5B(1-560)-L-GFP (KIF5B Human, Synthetic)
UseTagsEGFPExpressionMammalianMutationhsKIF5B(aa1-560);PromoterChicken beta-actinAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti Dest PuroR R1-R2
Plasmid#84575PurposeGateway destination vector expressing Puromycin resistance under a PGK promoterDepositorTypeEmpty backboneUseLentiviral; GatewayTagsExpressionMammalianMutationPromoterPGKAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
AktPH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax
Plasmid#173862PurposeMammalian expression of Akt PH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax (PIP3 marker and opto-PI3K)DepositorInsertAktPH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax
UseTagsC-terminal CAAX-box and mCherryExpressionMammalianMutationPromoterCAGAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-ACTB_sgRNA
Plasmid#183885PurposepX459V2.0-HypaCas9 plasmid with ACTB sgRNA for N-terminal tagging of beta-actin in human cells.DepositorInsertACTB sgRNA spacer (ACTB Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
phage - ubc - nls - 2xmcp - egfp - BirA*
Plasmid#131132PurposeBirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.DepositorInsertNLS-HA-2xMCP-EGFP-BirA*
UseLentiviralTagsNLS, HAExpressionMammalianMutationBirA* = BIrA >R118GPromoterhuman ubiquitin C (UBC)Available sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLXSN-H-Ras_N17
Plasmid#39537DepositorInsertHRAS N17 (HRAS Human)
UseRetroviralTagsExpressionMammalianMutationN17PromoterAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
EHD2-T72A-mEGFP
Plasmid#45934Purposedominant-negative EHD2 mutantDepositorInsertEps-15 homology domain-containing protein2 (EHD2 Human)
UseTagsmEGFPExpressionMammalianMutationT 72 changed to A (T72A)PromoterCMVAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
S142D drebrin-YFP
Plasmid#58336Purposemammalian expression of human drebrin-YFP phospho-mimetic mutantDepositorInsertdrebrin (DBN1 Human)
UseTagsEYFPExpressionMammalianMutationS142D, phospho-mimetic mutantPromoterCMVAvailable sinceAug. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
S142A drebrin-YFP
Plasmid#58335Purposemammalian expression of human drebrin-YFP phospho-dead mutantDepositorInsertdrebrin (DBN1 Human)
UseTagsEYFPExpressionMammalianMutationS142A, phospho-dead mutantPromoterCMVAvailable sinceAug. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28b_RanT24N
Plasmid#186271PurposeExpression vector of His-RanT24N in E.coliDepositorInsertGTP-binding nuclear protein Ran (Ran Mouse)
UseTagsHis tagged and His-tagged at N terminalExpressionBacterialMutationChanged T24 into NPromoterT7 promoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
M3-PSCFP2
Plasmid#114185Purposestudying clustering of M3 receptors by superresolution microscopy (PALM)DepositorInsertMuscarin acetylcholine receptor 3 (CHRM3 Human)
UseTags3xHA and PS-CFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pB80-KIF1A(1-383)-GFP-SSPB(milli)
Plasmid#174626PurposeConstitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(milli)DepositorInsertKIF1A(1-383)-GFP-SSPB(milli) (Kif1a Mouse, Synthetic)
UseTagsGFP-SSPB(milli)ExpressionMammalianMutationmmKIF1A(aa1-383): Pro202Ala; EGFP: Met1Del; SSPB:…PromoterChicken beta-actinAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-EPLIN alpha delta LIM
Plasmid#40933DepositorInsertEPLIN alpha (LIMA1 Human)
UseTagsFlagExpressionMammalianMutationLIM domain deleted- amino acids 221-295PromoterAvailable sinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits only