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Plasmid#140565PurposeExpresses Cas9-HF1 in mammalian cellsDepositorInsertCas9
UseTagsExpressionMutationN497A R661A Q695A Q926APromoterAvailable sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMM441
Plasmid#61271PurposeAlso referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.DepositorInsertCas9, tracrRNA, crRNA
UseCRISPRTags6xHisExpressionBacterialMutationPromoterAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
AB65_pCAG.Cas9-D10A.rBGpA
Plasmid#199256PurposeExpression construct encoding the SpCas9-D10A nickaseDepositorInsertSpCas9.D10A nickase
UseCRISPRTagsSV40 NLSExpressionMammalianMutationD10APromoterCAG promoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRU323
Plasmid#167690PurposeFinal destination vector for single Gateway LR reactionDepositorInsertPcUBQ4-2::Cas9i
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAE03
Plasmid#61044PurposeTo construct C-terminal GFP fusions, single crossover integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsGFP+ExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRU319
Plasmid#167686PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::spCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX165-Sniper-Cas9
Plasmid#140560PurposeExpresses Sniper Cas9 in mammalian cellsDepositorInsertCas9
UseTagsExpressionMutationF539S M763I K890NPromoterAvailable sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRU292
Plasmid#167687PurposeFinal destination vector for single Gateway LR reactionDepositorInsertPcUBQ4-2::Cas9i
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMM178
Plasmid#61270PurposeAlso referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.DepositorInsertCas9, tracrRNA, crRNA
UseCRISPRTags6xHisExpressionBacterialMutationPromoterpLtetO, native, native (respectively)Available sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
Eef1a-1955/-1>nls::Cas9::nls
Plasmid#59987PurposeEef1a (EF1alpha) promoter driving nls::Cas9::nlsDepositorInsertnls::Cas9::nls
UseCRISPRTagsExpressionMutationReverted mutations from dCas9 to wiltdtypePromoterCiinte.Eef1a (EF1alpha) -1955/-1Available sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CibN-dCas9
Plasmid#60551PurposeExpresses CibN-dCas9 in mammalian cellsDepositorInsertCibN-dCas9
UseCRISPRTagsFLAG and SV40 NLSExpressionMammalianMutationinactivating Cas9 mutations D10A and H840APromoterCMVAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRU52
Plasmid#167678PurposeFinal destination vector for single Gateway LR reactionDepositorInsertPcUB4-2::spCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU51
Plasmid#167677PurposeFinal destination vector for single Gateway LR reactionDepositorInsertPcUB4-2::spCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-HiFi-nCas9n-A72
Plasmid#125646Purposemicro-injection of poly-adenylated HiFi-nCas9n RNADepositorInsertHiFi-nCas9n
UseRna in vitro transcription vectorTagsExpressionMutationR691A from wild-type Cas9PromoterT3Available sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSL690
Plasmid#47753PurposeExpresses dCas9-VP64 fusionDepositorInsertdCas9-VP64
UseCRISPRTags3x FLAG, NLS, and VP64ExpressionMammalianMutationD10A and H840A mutations in Cas9PromoterCMVAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pX165-xCas9
Plasmid#140568PurposeExpresses xCas9 in mammalian cellsDepositorInsertCas9
UseTagsExpressionMutationA262T R324L S409I E480K E543D M694I E1219VPromoterAvailable sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
cr3Dd
Plasmid#184862Purposecr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and a species-specific recombination systemDepositorInsertCas9, bet, exo
UseTagsCas9 ssrA degradation tagExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBBK12 pCAS-Tyr-[gRNA: 2=FF21] (SplitKanR, AmpR)
Plasmid#178996PurposeSp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitKanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK11 pCAS-Tyr-[gRNA: 1=FF18] (SplitKanR, AmpR)
Plasmid#178995PurposeSp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitKanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21b - S. thermophilus DltB
Plasmid#132607Purposeexpresses S. thermophilus DltB in E. coliDepositorInsertDltb
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only