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pFBOH-MHL_AAMP:82-434

Plasmid

#210904

Purpose

Insect expression of human AAMP fragment D82 to R434. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

AAMP:D82-R434 (AAMP Human)

Use

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL_LRRK2:2141-2527

Plasmid

#210899

Purpose

Insect expression of human LRRK2 fragment T2141 to E2527. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

LRRK2:T2141-E2527 (LRRK2 Human)

Use

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

M2397T natural variant ( VAR_024965)

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pFBOH-LIC_DDB1:1-1140_delta:396-689

Plasmid

#210897

Purpose

Insect expression of full-length human DDB1 with a loop deletion I396 to D689. Untagged.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DDB1:M1-H1140_delta:I396-D689 (DDB1 Human)

Use

Tags

Expression

Insect

Mutation

loop deletion amino acids I396 to D689

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL_COP1:386-731

Plasmid

#210887

Purpose

Insect expression of human COP1 fragment A386 to V731. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

COP1:A386-V731 (COP1 Human)

Use

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pFBOH-LIC_DDB1:1-1140

Plasmid

#210886

Purpose

Insect expression of full-length human DDB1. Untagged.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DDB1:M1-H1140 (DDB1 Human)

Use

Tags

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL_WDR91:392-747_delta:522-536

Plasmid

#210881

Purpose

Insect expression of human WDR91 fragment P392 to A747 with loop deletion Q522 to N536. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WDR91:P392-A747_delta:522-536 (WDR91 Human)

Use

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

loop deletion amino acids Q522 to N536

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL_CDC20:161-477

Plasmid

#210943

Purpose

Insect expression of human CDC20 fragment S161 to P477. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CDC20:S161-P477 (CDC20 Human)

Use

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pFBOH-LIC_GRWD1:1-446

Plasmid

#210876

Purpose

Insect expression of full-length human GRWD1. N-terminal 6X His tag with thrombin protease cleavage site.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

GRWD1:M1-V446 (GRWD1 Human)

Use

Tags

MGSSHHHHHHSSGLVPRGS

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_CDC20:1-499

Plasmid

#211062

Purpose

Mammalian expression of full-length human CDC20. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CDC20:M1-R499 (CDC20 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_EIF2D:1-584

Plasmid

#211085

Purpose

Mammalian expression of full-length human EIF2D. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

EIF2D:M1-K584 (EIF2D Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_GEMIN5:1-739

Plasmid

#211124

Purpose

Mammalian expression of human GEMIN5 fragment M1 to A739. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

Gemin5:M1-A739 (GEMIN5 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

R682Q natural variant (VAR_033807)

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_DTL:1-730

Plasmid

#211060

Purpose

Mammalian expression of full-length human DTL. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DTL:M1-L730 (DTL Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNLF1-N_CDC40:1-579

Plasmid

#211061

Purpose

Mammalian expression of full-length human CDC40. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CDC40:M1-D579 (CDC40 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_EIF2B2:1-351

Plasmid

#211083

Purpose

Mammalian expression of full-length human EIF2B2. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

EIF2B2:M1-L351 (EIF2B2 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNLF1-C_EXOSC6:1-272

Plasmid

#211084

Purpose

Mammalian expression of full-length human EXOSC6. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

EXOSC6:M1-P272 (EXOSC6 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_LRRK2:2142-2527

Plasmid

#211112

Purpose

Mammalian expression of human LRRK2 fragment R2142 to E2527. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

LRRK2:R2142-E2527 (LRRK2 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

M2397T natural variant (VAR_024965)

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNLF1-N_CDC20:1-499

Plasmid

#211058

Purpose

Mammalian expression of full-length human CDC20. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CDC20:M1-R499 (CDC20 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNLF1-C_BTRC:1-605

Plasmid

#211063

Purpose

Mammalian expression of full-length human BTRC. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

BTRC:M1-R605 (BTRC Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNLF1-C_CBFA2T3:1-615

Plasmid

#211070

Purpose

Mammalian expression of full-length human CBFA2T3. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CBFA2T3:M1-R615 (CBFA2T3 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

not annotated isoform

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHTC-HaloTag_FBXO3:1-471

Plasmid

#211072

Purpose

Mammalian expression of full-length human FBXO3. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

FBXO3:M1-F471 (FBXO3 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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