169,069 results

1. mini-donor - NKX6.1-nEGFP

   Plasmid

   #206047

   Purpose

   this mini-donor knockin vector can be used to knock nuclear EGFP into NKX6.1 locus

   Depositor

   Xiaojun Lian

   Article

   Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

   Insert

   nuclear EGFP
   

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. #2.4 pre-G-SynapShot-Myc

   Plasmid

   #214722

   Purpose

   Expresses green #2.4 pre-SynapShot and Myc tag

   Depositor

   Won Do Heo

   Article

   Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

   Insert

   #2.4 pre-G-SynapShot
   

   Use

   AAV

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRL_GI_PCB28

   Plasmid

   #98745

   Purpose

   For phycocyanobilin production in Saccharomyces cerevisiae. Contains integrative cassette for the yeast his1-Δ200 locus. Encodes constitutively expressed HY1 and PcyA.

   Depositor

   Bernd Müller-Röber

   Article

   Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919

   Inserts

   heme oxygenase 1 (TED4 Mustard Weed)
   Phycocyanobilin:ferredoxin oxidoreductase
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Mutation

   Codon optimized for Saccharomyces cerevisiae

   Promoter

   ADH1m and PGK1

   Available Since

   Aug. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQAT13

   Plasmid

   #223385

   Purpose

   T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   AtUBQ10-hA3A-Y130F-SpCas9-D10A-UGI-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. T2A 3xNLS GFP

   Plasmid

   #227721

   Purpose

   Plasmid contains T2A 3xNLS GFP, with some nlp-51 homology sequence before, Amp Resistant

   Depositor

   Oliver Hobert, Haosheng Sun

   Article

   Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

   Insert

   T2A 3xNLS GFP with some nlp-51 homology sequence (nlp-51 Nematode)
   

   Use

   Cloning vector

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEcRNAP6

   Plasmid

   #128940

   Purpose

   pET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, Z

   Depositor

   Seth Darst

   Article

   Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:

   Inserts

   RpoA (rpoA E. coli)
   RpoB
   RpoC-ppx(His)10
   RpoZ
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   PreScission protease site (GE Healthcare; LEVLFQG…

   Promoter

   T7

   Available Since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLCS110

   Plasmid

   #209331

   Purpose

   Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mStayGold fusion protein.

   Depositor

   Lucia Strader

   Article

   Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. ATG-3925 (CBG2)

   Plasmid

   #167839

   Purpose

   Expresses CBG2 (Click Beetle Green 2) in E. coli and mammalian cells

   Depositor

   Lance Encell

   Article

   Zambito et al iScience. 2020 Dec 26;24(1):101986. doi:

   Insert

   Click Beetle Green 2
   

   Tags

   6-His

   Expression

   Bacterial and Mammalian

   Mutation

   Q348H. Click Beetle Green 2 (CBR2) has 3 amino a…

   Promoter

   CMV, T7

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-Flag-RBM24

   Plasmid

   #208052

   Purpose

   Expressing N-ter Flag tagged human RBM24 (ENST00000379052) in mammalian cells

   Depositor

   Quanfu Ma

   Insert

   human RBM24 (ENST00000379052) coding region (RBM24 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMA7-SacB

    Plasmid

    #79968

    Purpose

    TM-MAGE strain

    Depositor

    Morten Sommer

    Article

    Lennen et al Nucleic Acids Res. 2016 Feb 29;44(4):e36

    Inserts

    Lambda Red recombinase beta subunit
    DNA adenine methylase
    Levansucrase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Sept. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYES2-Ub-G76V-GFP

    Plasmid

    #11954

    Depositor

    Nico Dantuma

    Article

    Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

    Insert

    Ub-G76V-GFP
    

    Expression

    Yeast

    Mutation

    Ubiquitin fused to N-terminus of GFP. Glycine 76 …

    Available Since

    May 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ166

    Plasmid

    #109328

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter

    Depositor

    Yiping Qi

    Article

    Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

    Insert

    zCas9
    

    Use

    CRISPR; Gateway compatible zcas9 entry clone

    Tags

    3x FLAG, NLS and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAD-LyseR (in lacZ deficient strain)

    Plasmid

    #99245

    Purpose

    For autolysate production in lacZ deficient (lac operon deletion) E. coli BL21-Gold-dLac (DE3) strain: constitutively expresses phage lambda endolysin (gene R)

    Depositor

    Jeff Hasty

    Article

    Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

    Insert

    phage lambda gene R (R phage lambda)
    

    Expression

    Bacterial

    Promoter

    beta-lactamase promoter

    Available Since

    Oct. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-4T1_4xUb

    Plasmid

    #199779

    Purpose

    Used for the expression of 4X human ubiquitin in tandem (SMC interal no. 538)

    Depositor

    Sascha Martens

    Article

    Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

    Insert

    4X Ubiquitin in Tandem
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSHDY

    Plasmid

    #137661

    Purpose

    Conjugative vector with streamlined cloning capabilities. Contains two cloning sites for modular insertion of genetic parts.

    Depositor

    Ilka Axmann

    Article

    Behle et al ACS Synth Biol. 2020 Apr 17;9(4):843-855

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQ220 (AsCpf1)

    Plasmid

    #86208

    Purpose

    AsCpf1 Gateway entry plasmid

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Insert

    AsCpf1
    

    Use

    Gateway compatible cpf1 vectors

    Tags

    5' and 3' NLS

    Expression

    Plant

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSRK2721

    Plasmid

    #78572

    Purpose

    Gateway destination vector for production of MBP-3C site-His6-TEV site-passenger protein fusions

    Depositor

    David Waugh

    Article

    Raran-Kurussi et al Anal Biochem. 2016 Apr 19;504:30-37. doi

    Insert

    Gateway cloning cassette
    

    Expression

    Bacterial

    Available Since

    Sept. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221-SLC20A1_STOP

    Plasmid

    #161176

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC20A1 (SLC20A1 Human)
    

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pRK5_mCherry-GFP-nb-KS

    Plasmid

    #238260

    Purpose

    For overexpression of mCherry-GFP-nb-KS

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry-GFP-nb-KS
    

    Expression

    Mammalian

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFW2100

    Plasmid

    #224212

    Purpose

    Bacteroides genomic editing tool; IPTG inducible-Fncpf1

    Depositor

    Ophelia Venturelli

    Article

    Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

    Insert

    Fncpf1
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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