168,756 results

1. pAAV-CaMKIIa(0.4)-PdCO-mScarlet-WPRE

   Plasmid

   #198508

   Purpose

   Expresses optimized PdCO in frame with mScarlet under control of minimal CamKIIa promotor.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Insert

   PdCO
   

   Use

   AAV

   Tags

   Rho1D4 and mScarlet

   Expression

   Mammalian

   Promoter

   CaMKIIα minimal promotor (0.4kb)

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. FUW-TIE1-Cherry

   Plasmid

   #119236

   Purpose

   Plasmid encodes full-length human Tie1 gene fused in-frame with Cherry cloned in lentiviral vector.

   Depositor

   Kari Alitalo

   Insert

   TIE1-mCherry (TIE1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Promoter

   P hUbC

   Available Since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-tdTomato (codon diversified) (AAV Retrograde)

   Viral Prep

   #59462-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pAAV-CAG-tdTomato (codon diversified) (#59462). In addition to the viral particles, you will also receive purified pAAV-CAG-tdTomato (codon diversified) plasmid DNA. tdTomato expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Edward Boyden

   Promoter

   CAG

   Tags

   tdTomato (codon diversified)

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

4. Lenti-iCas9-neo

   Plasmid

   #85400

   Purpose

   Lentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequence

   Depositor

   Qin Yan

   Article

   Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

   Insert

   Flag-iCas9-P2A-GFP
   

   Use

   Lentiviral

   Tags

   Flag-iCas9-P2A-GFP

   Available Since

   Jan. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. SP-dCas9-VPR

   Plasmid

   #63798

   Purpose

   SP-dCas9 with VP64-p65-Rta (VPR) fused to C-terminus; mammalian vector

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

   Insert

   SP-dCas9-VPR
   

   Use

   CRISPR and Synthetic Biology

   Tags

   VPR (VP64-p65-Rta)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW57.1

   Plasmid

   #41393

   Purpose

   3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro

   Depositor

   David Root

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector, doxycycli…

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet ON

   Available Since

   Nov. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. MP6

   Plasmid

   #69669

   Purpose

   Untargetted Bacterial Mutagenesis

   Depositor

   David Liu

   Article

   Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

   Insert

   dnaQ926 dam seqA emrR ugi CDA1
   

   Expression

   Bacterial

   Promoter

   E. coli PBAD

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV_hSyn1_nLightG (AAV9)

   Viral Prep

   #187179-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV_hSyn1_nLightG (#187179). In addition to the viral particles, you will also receive purified pAAV_hSyn1_nLightG plasmid DNA. Synapsin-driven expression of norepinephrine sensor nLightG. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Tommaso Patriarchi

   Article

   Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

   Promoter

   human Synapsin-1

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

9. pEF6-Acly

   Plasmid

   #70765

   Purpose

   Expresses mouse ATP-citrate lyase in mammalian cells

   Depositor

   Kathryn Wellen

   Article

   Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

   Insert

   ATP Citrate Lyase (Acly Mouse)
   

   Tags

   myc-epitope

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. ptfLC3

    Plasmid

    #21074

    Purpose

    Mammalian expression of rat LC3 fused to mRFP and EGFP

    Depositor

    Tamotsu Yoshimori

    Article

    Kimura et al Autophagy. 2007;3(5):452-60.

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Tags

    EGFP and mRFP

    Expression

    Mammalian

    Available Since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-DIO-EGFP (AAV5)

    Viral Prep

    #50457-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-hSyn-DIO-EGFP (#50457). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-EGFP plasmid DNA. hSyn-driven, Cre-dependent EGFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Promoter

    Syn

    Tags

    EGFP (Cre-dependent)

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

12. pT7-PE7 for IVT

    Plasmid

    #214813

    Purpose

    Template for in vitro transcription of PE7

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7
    

    Use

    Template for in vitro transcription

    Tags

    SV40 bpNLS and c-Myc NLS

    Promoter

    T7 (inactivated)

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAVS1-tet-iCas9-BFP2

    Plasmid

    #125519

    Purpose

    all-in-one-inducible Cas9

    Depositor

    Jason Moffat

    Article

    Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

    Insert

    AAVS1-TET-ON 3G-SpCas9-P2A-BFP2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEVOL_PylRS(WT)-tRNA

    Plasmid

    #223512

    Purpose

    PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

    Depositor

    Edward Lemke

    Article

    Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

    Inserts

    Pyrrolysyl-tRNA Synthetase (2 copies) (pylS Methanosarcina mazei)
    tRNA Pyl
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    araBAD, rrnB, and glnS and proK

    Available Since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti CMV GFP Puro (658-5)

    Plasmid

    #17448

    Purpose

    3rd gen lentiviral eGFP expression vector, CMV promoter, Puro

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Insert

    GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. B-95.ΔA

    Bacterial Strain

    #197933

    Purpose

    BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

    Depositor

    Kensaku Sakamoto

    Article

    Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

    Bacterial Resistance

    None

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

17. PiggyBac dCas9-VPR

    Plasmid

    #191269

    Purpose

    piggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFP

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Inserts

    VPR-dCas9-HA-tagBFP
    rtTA3-IRES-PURO
    

    Use

    CRISPR

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Available Since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. FH-TET1-pEF

    Plasmid

    #49792

    Purpose

    This plasmid expresses the full-length human TET1 gene with a Flag and HA tag.

    Depositor

    Anjana Rao

    Article

    Tahiliani et al Science. 2009 May 15;324(5929):930-5. Ep

    Insert

    Tet methylcytosine dioxygenase 1 (TET1 Human)
    

    Tags

    Flag and HA

    Expression

    Mammalian

    Promoter

    EF-1alpha

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLS-SceI

    Plasmid

    #137725

    Purpose

    Lentivirus reporter assay plasmid that contains a I-SceI site and a EGFP reporter gene.

    Depositor

    Nadav Ahituv

    Article

    Gordon et al Nat Protoc. 2020 Aug;15(8):2387-2412. do

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-Cre

    Plasmid

    #13775

    Purpose

    Mammalian expression of Cre recombinase from the broadly active CAG promoter/enhancer

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Cre
    

    Use

    Cre/Lox

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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