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Plasmid#162761PurposeExpressing dCas9 and sgRNA containg MTSa targeting centromeresDepositorInsertdCas9 and sgRNA(SL2-sgSat-MTSb)
UseTagsExpressionMammalianMutationdCas9(nuclease deactivated Cas9)PromoterU6/CMVAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
p168
Plasmid#62618PurposeePathBrick pETM6 vector encoding CsF3H, FaDFR, and DuLARDepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
mH2A2-CT-MYC
Plasmid#45170DepositorUseTagsHIS and MYCExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_VPR_EBFP2
Plasmid#167881PurposePiggyBac compatible plasmid expressing dCas9-VPRDepositorInsertdCas9-VPR
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDY-HA1-HA2-neo
Plasmid#109123PurposeFR cassette provider to introduce FR cassette to a BAC by recombineeringDepositorInsertsHA1
HA2
UseTagsExpressionMutationPromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-AktAR2
Plasmid#175013PurposePlasma membrane-targeted AktAR2.DepositorInsertLyn-mCherry-Akt-STOPS
UseTagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046799
Plasmid#145577PurposeBacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQStrep2-CRYM
Plasmid#31293DepositorInsertcrystallin, mu (CRYM Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPSP6+t
Plasmid#48149PurposeShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage SP6DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-FEN1DA
Plasmid#35028DepositorInsertFEN1DA (FEN1 Human)
UseAdenoviralTagsFlagExpressionMammalianMutationD181A--NUCLEASE DEAD POINT MUTANTPromoterCMVAvailable sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRN3P-HA-Suv4-20h2
Plasmid#86691PurposeFor transcription of of Suv4-20h2 mRNA preceded by an HA-tag in 5'DepositorInserthistone-lysine N-methyltransferase KMT5C (Kmt5c Mouse)
UseTagsHAExpressionMutationPromoterT3Available sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV8-cPE-N
Plasmid#183538PurposeDeliver a split compact Prime Editor in vivoDepositorInsertN-terminal fragment of SpCas9 nickase (H840A)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4136461
Plasmid#149538PurposeBacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTags6xHIS;Linker_GGSG;MBP;Linker_GGSG;Cleavable TEVExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSilencer2.1-U6-NL1F35
Plasmid#190830PurposeFor mammalian expression of NL1F35, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker.DepositorInsertNL1F35 (A Dual-Specificity aptamer targeting OGT and GFP)
UseAffinity Reagent/ Antibody and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGMC00014
Plasmid#172526Purposenon targeting sgRNADepositorInsertNon-targeting guide
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR SV40 L5-L2
Plasmid#62095PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and multiple cloning site module. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseMule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTYF-super-mfSST-0.3k-Venus-WPRE
Plasmid#156393PurposeExpresses Venus under SST gene promoterDepositorInsertsVenus
Gal4p65
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
6xHis-TEV-UBC-9
Plasmid#87055PurposeExpresses 6xHis-tagged ubc-9, with a TEV protease cleavage site between the tag and the ORFDepositorInsertUBC-9 (ubc-9 Nematode)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFL-EGFP-3xFlag-DEST
Plasmid#134903PurposeBaculovirus co-expression of GFP and gateway cloned protein with 3x-N-terminal Flag tagDepositorTypeEmpty backboneUseTags3x-FlagExpressionInsectMutationPromoterpolyhedronAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPKm-202
Plasmid#90492PurposeExpresses pcDNA3 - CMVmin - 5X UAS - pFR - CMVmin LuciferaseDepositorInsertCMVmin Luciferase Fluc
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only