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Showing: 9321 - 9340 of 54891 results

  2. pYPQ265L2

    Plasmid
    #174000
    Purpose
    Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY for C-G base editing
    Depositor
    Yiping Qi
    Article
    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d
    Insert
    UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY
    Use
    CRISPR
    Tags
    3X FLAG, NLS
    Expression
    Plant
    Mutation
    In rAPOBEC1: R33A; in zCas9(D10A)-SpRY: D10A, A61…
    Promoter
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    174000-plasmid-map-sequence-id-343423

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  5. pSEVA251_ Ptrc.x.tetO2::D-HicDH

    Plasmid
    #185456
    Purpose
    CDS of D-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 under the control of the synthetic promoter Ptrc.x.tetO2 and the RBS BBa_B0030 in pSEVA251 plasmid.
    Depositor
    Wolfgang Kroutil
    Article
    Jurkaš et al Engineering Microbiology, 2022
    Insert
    D-HicDH from Lactobacillus paracasei DSM 20008
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Ptrc.x.tetO2
    Available since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    185456-plasmid-map-sequence-id-367933

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  9. pCSJ982

    Plasmid
    #176622
    Purpose
    Express yeast Ub-MPGLW-Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.
    Depositor
    Alexander Varshavsky
    Article
    Dong et al Proc Natl Acad Sci U S A. 2020 Jun 23;11
    Insert
    Ub-MPGLW-Fbp1
    Use
    Tags
    Ubiquitin N-terminal fusion and triple flag tag C…
    Expression
    Yeast
    Mutation
    Changed first 4 N-terminal amino acid residues of…
    Promoter
    TDH3tc3
    Available since
    March 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    176622-plasmid-map-sequence-id-356527

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  16. pCAG-mSpata33 (I81A)-PA

    Plasmid
    #179131
    Purpose
    Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. I81A mutation is introduced in Spata33.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11
    Insert
    spermatogenesis associated 33 (Spata33 Mouse)
    Use
    Tags
    PA
    Expression
    Mammalian
    Mutation
    Changed Isoleucine 81 to Alanine
    Promoter
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179131-plasmid-map-sequence-id-352818

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  17. pCAG-mSpata33 (P79A)-PA

    Plasmid
    #179130
    Purpose
    Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. P79A mutation is introduced in Spata33.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11
    Insert
    spermatogenesis associated 33 (Spata33 Mouse)
    Use
    Tags
    PA
    Expression
    Mammalian
    Mutation
    Changed Proline 79 to Alanine
    Promoter
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179130-plasmid-map-sequence-id-352804

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  18. pCAG-mSpata33 (I83A)-PA

    Plasmid
    #179132
    Purpose
    Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. I83A mutation is introduced in Spata33.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11
    Insert
    spermatogenesis associated 33 (Spata33 Mouse)
    Use
    Tags
    PA
    Expression
    Mammalian
    Mutation
    Changed Isoleucine 83 to Alanine
    Promoter
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179132-plasmid-map-sequence-id-352797

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Showing: 9321 - 9340 of 54891 results