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Showing: 9301 - 9320 of 24713 results

  1. Burden Monitor lambda version

    Plasmid
    #66073
    Purpose
    The burden monitor was cloned inside plasmid pAH63 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cells
    Depositor
    Tom Ellis
    Article
    Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:
    Insert
    GFP
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 21, 2015
    Availability
    Academic Institutions and Nonprofits only

  3. PX458 V3

    Plasmid
    #226957
    Purpose
    CBh-SpCas9-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al bioRxiv 2024.07.19.604224
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226957-plasmid-map-sequence-id-450777

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  4. pTRIPZ-Myr-PTEN

    Plasmid
    #206983
    Purpose
    Lentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with myristyolation signal
    Depositor
    Ming Chen
    Article
    Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:
    Insert
    PTEN (PTEN Human)
    Use
    Lentiviral
    Tags
    N-terminal myristoylation site
    Expression
    Mammalian
    Mutation
    Promoter
    tet-on promoter
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    206983-plasmid-map-sequence-id-408428

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  9. pK7WGF2::hCas9

    Plasmid
    #46965
    Purpose
    Expesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue
    Depositor
    Sophien Kamoun
    Article
    Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.
    Insert
    hCas9
    Use
    CRISPR; Plant expression
    Tags
    eGFP
    Expression
    Mutation
    Promoter
    35S
    Available since
    Aug. 16, 2013
    Availability
    Academic Institutions and Nonprofits only
    46965-plasmid-map-sequence-id-66887

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  10. pRelA'

    Plasmid
    #175595
    Purpose
    Tet inducible, KAN Resistant, non labelled synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.
    Depositor
    Greg Bokinsky
    Article
    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.
    Insert
    Catalytic domain of E.coli relA gene.
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Only the catalytic domain of the enzyme is used (…
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    175595-plasmid-map-sequence-id-348284

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Showing: 9301 - 9320 of 24713 results