Addgene

We narrowed to 28,087 results for: eng

Showing: 9281 - 9300 of 28087 results

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pGEL089

Plasmid

#137897

Purpose

iSTU-CRISPR/Cas12a HH-HDV structure crRNA insertion vector with OsCDPK2 intron

Depositor

Yong Zhang

Article

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pc3XB-ZF10

Plasmid

#13109

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF10

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pOVC1_L3(GSSSSGGSSSG)

Plasmid

#131210

Purpose

Cloning vector containing A-B-C casette with linker L3 in site B

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

GSSSSGGSSSG llinker

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1_L2(GSSGGSSG)

Plasmid

#131209

Purpose

Cloning vector containing A-B-C casette with linker L2 in site B

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

GSSGGSSG linker

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1_L1(GSSSG)

Plasmid

#131208

Purpose

Cloning vector containing A-B-C casette with linker L1 in site B

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

GSSSG linker

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pTRANS_130

Plasmid

#91204

Purpose

Transformation, Type: non-T-DNA, Plant Selection: 2x35S:bar, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pJB073

Plasmid

#98912

Purpose

Express Dronpa tagged ZapB in E coli

Depositor

Jie Xiao

Article

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Insert

ZapB

Use

Tags

Dronpa

Expression

Bacterial

Mutation

Promoter

T5-lac

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 RAP2A

Plasmid

#67058

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

RAP2A (RAP2A Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
Lifeact-rsLOV1 pcDNA3.1(+)

Plasmid

#107881

Purpose

Mammalian expression of Lifeact-rsLOV1

Depositor

Stefan Hell

Article

Gregor et al Sci Rep. 2018 Feb 9;8(1):2724. doi: 10.1

Insert

rsLOV1

Use

Tags

Lifeact

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0007

Plasmid

#117534

Purpose

Level0 Golden Gate part: CDS, LbCas12a

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

LbCas12a (with stop codon)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pTRANS_202

Plasmid

#91106

Purpose

Transformation, Type: T-DNA, Plant Selection: none, Viral Replication: ToLCV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

No Promoter

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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PGRP-SA

Plasmid

#21055

Depositor

Johann Deisenhofer

Article

Chang et al PLoS Biol. 2004 Sep . 2(9):E277.

Insert

PGRP-SA (PGRP-SA Fly)

Use

Tags

His

Expression

Insect

Mutation

Promoter

Available since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pJZC548

Plasmid

#62316

Purpose

sgRNA with 1x PP7 for yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + 1x PP7

Use

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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TAL3030

Plasmid

#41204

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-bbc3-(puma)-Left (bbc3 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
pc3XB-ZF5

Plasmid

#13104

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF5

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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PLEXIN A3

Plasmid

#58238

Purpose

Riboprobe for in vitro transcription of Plexin A3

Depositor

Christiana Ruhrberg

Article

Schwarz et al Dev Biol. 2008 Dec 1;324(1):1-9. doi: 10

Insert

Plexin A3 (Plxna3 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pBDKRB2_TCS(Q’L)_HA-dCas9(N)_DHFR

Plasmid

#101109

Purpose

Encodes dCas9(N)-DHFR fused to BDKRB2

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

BDKRB2-dCas9(N)-DHFR

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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dSp-VP64-p65-RTA(75-190)

Plasmid

#99670

Purpose

Expresses dSp Cas9 fused to combination of truncated activation domains

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

VP64-p65-RTA(75-190)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 30, 2020

Availability

Academic Institutions and Nonprofits only

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pJK_proK6_eGFP

Plasmid

#59850

Purpose

Constitutively expresses eGFP with proK6 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

Enhanced Green Fluorescent Protein

Use

Tags

6x-His

Expression

Bacterial

Mutation

S2R,S65G,S72A

Promoter

proK6

Available since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pBW112pBAD-hrpV

Plasmid

#78639

Purpose

pSB3K3 carrying araC-PBAD

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

araC-PBAD

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

see insert

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only