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Addgene
Showing: 9221 - 9240 of 20645 results
  1. dCas9-p300 HAT

    Plasmid
    #179544
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) driven by AF4 promoter
    Depositor
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) (EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Availability
    Academic Institutions and Nonprofits only
  2. dCas9-p300 PHD-HAT

    Plasmid
    #179545
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 PHD-HAT domain (aa 1243-1664) driven by EF-1alpha promoter
    Depositor
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human p300 PHD-HAT domain (aa 1243-1664) (EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Availability
    Academic Institutions and Nonprofits only
  3. dCas9-p300 RING-PHD-HAT

    Plasmid
    #179546
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 RING-PHD-HAT domain (aa 1155-1664) driven by EF-1alpha promoter
    Depositor
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human p300 RING-PHD-HAT domain (aa 1155-1664) (EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Availability
    Academic Institutions and Nonprofits only
  4. pLX_TRC311/NLS-Cas13d-P2A-Blast

    Plasmid
    #212196
    Purpose
    CasRx (NLS-RfxCas13d-NLS) with 2A-Blasticidin for RNA targeting. CasRx-2A-Blasticidin is driven by EF1a promoter. EGFP is driven by SV40
    Depositor
    Insert
    CasRx (NLS-RfxCas13d-NLS)-HA with 2A-Blasticidin
    Use
    CRISPR and Lentiviral
    Tags
    HA, NLS, and blasticidin S deaminase
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α promoter
    Availability
    Academic Institutions and Nonprofits only
  5. Cas9 CFP

    Plasmid
    #155278
    Purpose
    Lentiviral Cas9 expression with CFP marker
    Depositor
    Insert
    spCas9
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  6. Cas9 NGFR

    Plasmid
    #155279
    Purpose
    Lentiviral Cas9 expression with NGFR marker
    Depositor
    Insert
    spCas9
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  7. pCR1

    Plasmid
    #154270
    Purpose
    CRISPR-targeted-plasmid
    Depositor
    Insert
    CR1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    CCG-AAG
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  8. PCMV-ABE8e-VRER

    Plasmid
    #198018
    Purpose
    A-to-G base editor
    Depositor
    Insert
    ABe8e-VRER
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  9. PB-iCas9

    Plasmid
    #160048
    Purpose
    inducible expression of Cas9
    Depositor
    Insert
    Cas9
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Availability
    Academic Institutions and Nonprofits only
  10. Lenti Cas9/SB100

    Plasmid
    #155294
    Purpose
    Lentiviral expression of Cas9 and SB100
    Depositor
    Insert
    SB100X-P2A-spCas9
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  11. pTJK459

    Plasmid
    #138008
    Purpose
    Empty sgRNA expression vector for expression of sgRNA for Sp Cas9
    Depositor
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    U-A flip and hairpin extension: cgtttAagagctaTGCT…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  12. pRAC01A

    Plasmid
    #193730
    Purpose
    Expresses deactivated-Cas9 (J23101) and tracrRNA, with BBR1 origin, ampR, Start-Stop assembly site for array insertion
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  13. pRAC07A

    Plasmid
    #193733
    Purpose
    Expresses deactivated-Cas9 (J23107) and tracrRNA, with BBR1 origin, ampR, Start-Stop assembly site for array insertion
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  14. pGMC00020 (aka pRC0431)

    Plasmid
    #195304
    Purpose
    Lentiviral expression vector derived from LEGO-G2 with blasticidin selection
    Depositor
    Type
    Empty backbone
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  15. pGMC00021 (aka pRC0432)

    Plasmid
    #195305
    Purpose
    Lentiviral expression vector derived from LEGO-G2 with puromycin selection
    Depositor
    Type
    Empty backbone
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  16. pGMC00022 (aka pRC0433)

    Plasmid
    #195321
    Purpose
    Lentiviral expression vector derived from LEGO-G2 with hygromycin selection
    Depositor
    Type
    Empty backbone
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 9221 - 9240 of 20645 results