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Plasmid#59545PurposeExpresses yeast Atg13 under the endogenous promoter with a GFP N-terminal tagDepositorInsertATG13 (ATG13 Budding Yeast)
UseTagsGFPExpressionBacterial and YeastMutationAvrII site created after start codonPromoterATG13Available sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTC-mcs
Plasmid#20317DepositorInsertmultiple cloning site
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-H2BsfGFP-tdTomato - opt GS PQR Var3 P2A -APGST E
Plasmid#113847PurposeHistone H2B-superfolder GFP and tdTomato linked by P2A (-APGST linker)DepositorInsertH2B-sfGFP-P2A-tdTomato
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHL1746
Plasmid#53034PurposePLtetO-1:ycdTlead::mCherry, PLlacO-1:RBS(st7):csrADepositorInsertsmCherry
csrA
UseSynthetic BiologyTagsRBS(st7) and ycdTleadExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
Py-MDR2-NBD
Plasmid#25590PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPy-MDR2-NBD
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-C
Plasmid#213496PurposeEncodes Node C of the three-node Turing circuitDepositorInsertslambda cI 5G6GP
mCherry
UseSynthetic BiologyTagsASV tag and LVA tagExpressionMutationPromoterAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:MCS+:NosT
Plasmid#212160PurposeThis binary vector has an empty backbone with extended multiple cloning site (MSC) where you can inserta a gene into a cassette with a strong promoter (truncated 35sCAMV) and UTR (Tomato Mosaic Virus)DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
p_L_3c_SG_RLuc
Plasmid#208729PurposeFor assessing leaky scanning rates in mammalian cellsDepositorInsertORF3c (ORF3a )
UseTagsRenilla luciferaseExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSB4A3-PBAD-phzSM
Plasmid#165620PurposeArabinose-inducible expression of phzSM for conversion of phenazine-1-carboxylic acid to pyocyaninDepositorUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJK323
Plasmid#73495PurposeProduces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes and N5-carboxyaminoimidazole ribonucleotide synthetaseDepositorInsertshypothetical protein
N5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK322
Plasmid#73494PurposeProduces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes and N5-carboxyaminoimidazole ribonucleotide mutaseDepositorInsertshypothetical protein
N5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationPromotern/aAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pHL1747
Plasmid#53035PurposePLtetO-1:ydeHlead::mCherry, PLlacO-1:RBS(st7):csrADepositorInsertsmCherry
csrA
UseSynthetic BiologyTagsRBS(st7) and ydeHleadExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL1722
Plasmid#53030PurposePLtetO-1:glgClead::mCherry, PLlacO-1:RBS(st7):csrADepositorInsertsmCherry
csrA
UseSynthetic BiologyTagsRBS(st7) and glgC leaderExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL1745
Plasmid#53033PurposePLtetO-1:cstAlead::mCherry, PLlacO-1:RBS(st7):csrADepositorInsertsmCherry
csrA
UseSynthetic BiologyTagsRBS(st7) and cstAleadExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL1724
Plasmid#53031PurposePLtetO-1:pgaAleadFull::mCherry, PLlacO-1:RBS(st7):csrADepositorInsertsmCherry
csrA
UseSynthetic BiologyTagsRBS(st7) and pgaAleadFullExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL1928
Plasmid#53043PurposePLtetO-1:Agn43RBS:mCherry, PLlacO-1:RBS(st7):csrADepositorInsertsmCherry
csrA
UseSynthetic BiologyTagsAgn43RBS and RBS(st7)ExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL1914
Plasmid#53042PurposePconNoHindM2:hfqlead::gfp, PconNoHind:RBS(st3):tetRDepositorInsertsGFP
tetR
UseSynthetic BiologyTagsRBS(st3) and hfqleadExpressionBacterialMutationPromoterPconNoHindM2 and pLlacO-1Available sinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPROBE-NT
Plasmid#37818DepositorInsertPromotorless gfp reporter gene
UseTagsExpressionMutationPromoterAvailable sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-LMO11 (GeNL_SS-ChRmine)
Plasmid#213034Purposefusion protein of mNeonGreen, SSLuc, and ChRmine for bioluminescent optogeneticsDepositorInsertsmNeonGreen
ISN-IE
ChRmine
UseAAVTagsGaussia luciferase signal sequenceExpressionMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only