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pET22b-ecDHFR-N23PP/G51PEKN

Plasmid

#118583

Purpose

Bacterial expression of E.coli DHFR N23PP and G51PEKN mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Insert

DHFR

Use

Tags

Expression

Bacterial

Mutation

N23 mutated to P23, G51 mutated to P51, a Pro res…

Promoter

T7

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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4E2H.210_LUMABS

Plasmid

#155209

Purpose

Expresses 4E2H.210_LUMABS sensor in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4E2H.210_LUMABS

Use

Tags

6x His Tag

Expression

Mutation

Promoter

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pX330_sgACTA2

Plasmid

#63712

Purpose

Express sgACTA2 in mammalian cells.

Depositor

Stanley Qi

Article

Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

Insert

ACTA2 (ACTA2 Mouse, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pUDE714

Plasmid

#103021

Purpose

expression of a Cpf1 programming crRNA targetting HIS4 (crHIS4-4.S)

Depositor

Jean-Marc Daran

Article

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

crHIS4-4.S (HIS4 Budding Yeast)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pET20b-delta133HtrA2 S400D

Plasmid

#16156

Depositor

L. Miguel Martins

Article

Plun-Favreau et al Nat Cell Biol. 2007 Sep 30. ():.

Insert

HtrA2 (HTRA2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

S400D

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
49535-sgFMR1_E17A

Plasmid

#157782

Purpose

Expresses a sgRNA targeting stop codon of human FMR1 gene

Depositor

Xinyu Zhao

Article

Li et al Genome Res. 2020 Mar;30(3):361-374. doi:

Insert

FMR1 (FMR1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAV10.F3

Plasmid

#63199

Purpose

BioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pET20b-delta133HtrA2 S142A

Plasmid

#16153

Depositor

L. Miguel Martins

Article

Plun-Favreau et al Nat Cell Biol. 2007 Sep 30. ():.

Insert

HtrA2 (HTRA2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

S142A

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
CDK4-luciferase active (880)

Plasmid

#86657

Purpose

Luciferase reporter containing CDK4 promoter

Depositor

Jaime Modiano

Article

Baksh et al Mol Cell. 2002 Nov;10(5):1071-81.

Insert

CDK4 promoter (CDK4 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

867 base pairs including up to coding nucleotide …

Promoter

CDK5

Available since

June 20, 2017

Availability

Academic Institutions and Nonprofits only

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pet28a_RBPMS_FL

Plasmid

#59391

Purpose

Plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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Thy-Kv2.1-HA

Plasmid

#111541

Purpose

For expression of human KCNB1 in mouse brain

Depositor

Federico Sesti

Article

Yu et al J Neurosci. 2016 Oct 26;36(43):11084-110

Insert

thy1.2-Kv2.1-HA (KCNB1 Human)

Use

Mouse Targeting

Tags

HA

Expression

Mutation

Promoter

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-Barkor (∂CC)

Plasmid

#24404

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Barkor (ATG14 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of amino acids 91-180 of the coiled-coil…

Promoter

Available since

May 20, 2010

Availability

Academic Institutions and Nonprofits only
pSN67

Plasmid

#100124

Purpose

expresses putative KLF4 DBD with a 6xHis tag

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCherry-P2A-putative KLF4 DBD with a 6xHis tag (KLF4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Insert corresponds to the DBD of KLF4

Promoter

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-PTEN-deltaPDZ

Plasmid

#110182

Purpose

Expression of a mutated PTEN lacking its 4 last aa, and tagged with EGFP

Depositor

Jose Esteban

Article

Jurado et al EMBO J. 2010 Aug 18;29(16):2827-40. doi:

Insert

PTEN (Pten Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of last 4 amino acids

Promoter

CMV

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET20b-delta133HtrA2 S142D

Plasmid

#16154

Depositor

L. Miguel Martins

Article

Plun-Favreau et al Nat Cell Biol. 2007 Sep 30. ():.

Insert

HtrA2 (HTRA2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

S142D

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
p1585588

Plasmid

#62620

Purpose

ePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLAR (1:3:3 Ratio)

Depositor

Mattheos Koffas

Article

Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

Inserts

flavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
dihydroflavonol 4-reductase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
leucoanthocyanidin reductase

Use

Synthetic Biology

Tags

GBD, PDZ, and SH3

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pVL90 - VND7

Plasmid

#116035

Purpose

entry vector for GreenGate cloning method, CDS

Depositor

Thomas Greb

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

VND7 (VND7 Mustard Weed)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pET20b-delta133HtrA2 S400A

Plasmid

#16155

Depositor

L. Miguel Martins

Article

Plun-Favreau et al Nat Cell Biol. 2007 Sep 30. ():.

Insert

HtrA2 (HTRA2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

S400A

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pCMV-myc-Sema4DdeltaC

Plasmid

#51602

Purpose

expresses Sema4D lacking C terminus

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

Sema4DdeltaC (Sema4d Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Last 69 amino acids of Sema4D C-terminus are dele…

Promoter

CMV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
LentiCRISPRv2-sgSORCS2-G2

Plasmid

#118416

Purpose

CRISPR knockout, expresses Cas9, and sgRNA targeting Human SORCS2

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

gRNA SORCS2 Human (SORCS2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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