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Plasmid#89928PurposeExpresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.DepositorInserthuman insulin-Gaussia-Luciferase (INS Human, Synthetic)
UseTagsExpressionMammalianMutationDNA sequence for Gaussia luciferase was humanized…Promoterrat Insulin II promoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-peroxi
Plasmid#137806PurposeVisualization of peroxisomesDepositorArticleInsertSKL
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
LV-indLS2
Plasmid#123068PurposeLentiviral construct that delivers a tamoxifen inducible Cre. Upon recombination, luc2 and mStrawberry express. Used to image spontaneous tumorigenesis or subclonal cell populations in vivo.DepositorInsertsCreERT2 with intron
Firefly luciferase
mStrawberry
UseLentiviral and Luciferase; FluorescenceTagsExpressionMammalianMutationsynthetic intron added as indicatedPromoterCAGGS (after Cre mediated inversion) and CAGGS (f…Available sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
Neurofascin-186-HA
Plasmid#31061DepositorInsertNeurofascin (Nfasc Rat)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMarch 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pMRX-IZ-ANXA7-mRuby3
Plasmid#184893PurposeStable expression of ANXA7-mRuby3 (Annexin A7-mRuby3) in mammalian cellsDepositorInsertANXA7 (ANXA7 Human)
UseTagsmRuby3ExpressionMammalianMutationPromoterAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
epi-BE4max-NG
Plasmid#135977PurposeExpresses AncBE4max(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication originDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Rab9a
Plasmid#78592PurposeExpress mCherry-Rab9a in mammalian cellsDepositorInsertRab9a (RAB9A Human)
UseTagsmCherry2ExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 CB1
Plasmid#13391PurposeExpresses mouse cannabinoid receptor 1 (CB1) in mammalian cellsDepositorInsertCB1 (Cnr1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-EGFP
Plasmid#50473PurposeEGFP under the control of GFAP promoterDepositorInsertEGFP
UseAAVTagsN/AExpressionMutationPromoterGFAPAvailable sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-12 ONE-GO
Plasmid#189739PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG SMG1 WT
Plasmid#199573PurposeExpresses FLAG-tagged WT SMG1DepositorInsertSMG1 (SMG1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_EPHB2
Plasmid#187797PurposeMAC-tagged gene expression of human EPHB2DepositorInsertEPHB2 (EPHB2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEX.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP
Plasmid#45565PurposeExpresses I-Sce I nuclease in mammalian cells and BFP as a trackerDepositorInsertsI-Sce I Nuclease
BFP
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAG426GPD-ccdB-yeastSMASh-YFP
Plasmid#68854PurposeExpresses SMASh tag fused to YFP at N terminus in yeastDepositorInsertyeastSMASh-YFP
UseTagsSMASh tagExpressionYeastMutationPromoterGPDAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
MDH1-PGK-H2Kb2
Plasmid#17852DepositorInsertH-2Kb (H2-K1 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pETM6-E12-vioABECD
Plasmid#66537PurposepETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)DepositorInsertsVioA
VioB
VioE
VioC
VioD
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterMutant T7 Promoter - G6 and Mutant T7 Promoter - …Available sinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CBE4max-SpRYc-P2A-EGFP
Plasmid#208336PurposeCAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYcDepositorInserthuman codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationSc++ (1-1119), SpRY (1111-1368), D10APromoterCAGAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK5 T204D
Plasmid#80877Purposemammalian expression of constitutively active ALK5 T204DDepositorInsertALK5 (TGFBR1 Human)
UseTagsHAExpressionMammalianMutationT204D (constitutively active)PromoterCMVAvailable sinceAug. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-Luc-N
Plasmid#58790PurposeExpress LucN alone in mammalian cellsDepositorInsertfirefly Luciferase 1-398
UseTagsFLAGExpressionMammalianMutationT576C,T591C to destroy MfeI and EcoRI sitesPromoterCMVAvailable sinceAug. 15, 2014AvailabilityAcademic Institutions and Nonprofits only