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pMSCV-puro-5A, 6A mMCL1-OM

Plasmid

#45818

Depositor

Joseph Opferman

Article

Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

Insert

5A, 6A mMCL-1-OM

Use

Tags

Expression

Mammalian

Mutation

Changed Arginine to Alanine at 5th and 6th amino …

Promoter

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only
pCMV-mGold-CAF1-C-10

Plasmid

#158003

Purpose

Visualization of nucleus in mammalian cells using mGold

Depositor

Francois St-Pierre

Article

Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

Insert

CAF1 (Chaf1a Mouse)

Use

Tags

mGold

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-A762V

Plasmid

#174795

Purpose

Bacterial expression of wild type PARP1 mutant (A762V is reversion of the common V762A polymorphism)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-A762V (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

A762V

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-p97-RH-mycStrep

Plasmid

#31839

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Use

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

R155H

Promoter

CMV

Available since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pGL-p21UTRm2

Plasmid

#20879

Depositor

Robert Blelloch

Article

Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

Insert

p21 3'UTR site 2 mutation (Cdkn1a Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Predicted miRNA binding site 2 (Position ~1100) G…

Promoter

Available since

March 12, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA-FLAG-NEMO-C54/347A

Plasmid

#27268

Depositor

Thomas Gilmore

Article

Herscovitch et al Biochem Biophys Res Commun. 2008 Feb 29.

Insert

NEMO (IKBKG Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Cys54Ala Cys347Ala

Promoter

Available since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only
HEL binding scFv D44.1

Plasmid

#111718

Purpose

Yeast Surface Display of D44.1 as a reference starting point for design

Depositor

Sarel Fleishman

Article

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

D44.1

Use

Tags

cmyc tag

Expression

Yeast

Mutation

Promoter

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Emerin

Plasmid

#120246

Purpose

Expresses V5-tagged Emerin in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Emerin (Emd Mouse)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

EF1a

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pVL94 - pOp4:VND7

Plasmid

#116016

Purpose

destination vector for GreenGate cloning method, "Effector line", crossed with a "Driver line" the GOI, here VND7, will be expressed upon Dex-induction

Depositor

Thomas Greb

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pOp4:B-dummy:VND7:VP16:tUBQ10::BastaR

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FluoSTEP-EKAR

Plasmid

#181858

Purpose

Genetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

FluoSTEP-EKAR

Use

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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49535-sgFMR1_E3B

Plasmid

#157783

Purpose

Expresses a sgRNA targeting the 3rd exon of human FMR1 gene

Depositor

Xinyu Zhao

Article

Li et al Genome Res. 2020 Mar;30(3):361-374. doi:

Insert

FMR1 (FMR1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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FnoCas12a-DR-cr in PBCSK-

Plasmid

#126642

Purpose

Cloning vector FnoCas12a DR-crRNA for expression in mammalian cells

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

FnoCas12a Full Length Direct Repeat crRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 Promoter

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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ins:MCS2; cryaa:RFP

Plasmid

#110286

Purpose

A zebrafish insulin promoter containing vector with a second multiple cloning sites. Contains red eye marker, and is flanked with I-SceI sites.

Depositor

Nikolay Ninov

Article

Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

Type

Empty backbone

Use

Zebrafish

Tags

Expression

Mutation

Promoter

insulin

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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LjUBQ:MtGLV6:T35s

Plasmid

#164741

Purpose

Overexpression of Peptide coding gene (Binary vector)

Depositor

Wolf Scheible

Article

Roy et al Plant J. 2024 May;118(3):607-625. doi: 1

Insert

Medtr3g095180.1

Use

Tags

Terminator 35S

Expression

Plant

Mutation

Promoter

Lotus japonicus Ubiquitin

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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LjUBQ:MtGLV2:T35s

Plasmid

#164740

Purpose

Overexpression of Peptide coding gene (Binary vector)

Depositor

Wolf Scheible

Article

Roy et al Plant J. 2024 May;118(3):607-625. doi: 1

Insert

Medtr5g012400.1

Use

Tags

Terminator 35S

Expression

Plant

Mutation

Promoter

Lotus japonicus Ubiquitin

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-hMED9

Plasmid

#15365

Depositor

Ronald Conaway, Joan Conaway

Article

Paoletti et al Proc Natl Acad Sci U S A. 2006 Dec 12. 1

Insert

hMED9 (MED9 Human)

Use

Tags

His

Expression

Bacterial

Mutation

N/A

Promoter

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
MO70-hJunctate - EF-mutant

Plasmid

#79597

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Srikanth et al Proc Natl Acad Sci U S A. 2012 May 29;10

Insert

junctate (ASPH Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

77DAD79 to 77AAA79

Promoter

CMV

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only
L309-myc-seipin-CT

Plasmid

#64063

Purpose

myc-tagged murine seipin (cytosolic C-terminus); lentiviral construct

Depositor

Weiping Han

Article

Yang et al Biochem J. 2013 May 15;452(1):37-44. doi

Insert

bscl2 (Bscl2 Mouse)

Use

Lentiviral

Tags

myc epitope

Expression

Mammalian

Mutation

Contains Arg326 to Ser443

Promoter

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only
dmBACCS2VL-IRES-dOrai-IRES-GFP

Plasmid

#72897

Purpose

Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2VL-IRES-dOrai-IRES-GFP

Use

Tags

IRES-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-p97-RG-mycStrep

Plasmid

#31840

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Use

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

R95G

Promoter

CMV

Available since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only