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Plasmid#89072Purposegenomic GBP-mCherry epitope N-terminal tagging at lys1+ locus in S. pombeDepositorTypeEmpty backboneUseGenomic integration in s. pombeTagsGBP-mCherryExpressionYeastMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only
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pUCHR-mClover-smAID-P2A-CD5HA2-bglpA
Plasmid#110655PurposeLentiviral vector expressing cell surface HA-tag and monomeric GFP C-terminaly fused with auxin inducible degronDepositorInsertmClover-AID-P2A-CD5HA2-bglpA
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-hph-Ptac-VSVG
Plasmid#218365PurposeEmpty backbone for Tn7-site insertion with lacIq-Ptac expression system and C-terminal VSVG fusionsDepositorTypeEmpty backboneUseTagsVSVGExpressionBacterialMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-HDRT-CD3z-truncCARgsg(anti-CD19)
Plasmid#215759PurposeHDR template to insert a truncated CD3-zeta-deficient CD19-CAR into CD247 exon 2 (enhanced expression by GSG-2A linker)DepositorUseTagsIgG1 CH3ExpressionBacterialMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated
Plasmid#215769Purposeencodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locusDepositorInsertsUseTagsExpressionBacterialMutationCas12a PAM mutatedPromoterAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-HDRT-CD3z-truncCAR(anti-CD19)
Plasmid#215758PurposeHDR template to insert a truncated CD3-zeta-deficient CD19-CAR into CD247 exon 2DepositorUseTagsIgG1 CH3ExpressionBacterialMutationPromoterAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Top2A-5X Gly-Venus-T2A-Hygro
Plasmid#211115PurposeFor tagging the C-term of Top2A with VenusDepositorInsertsUseCRISPRTagsExpressionBacterial and MammalianMutationPromotern/aAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTR-348 pUC19-tNGFR-P2A-ICOS N
Plasmid#186066PurposeKnockin of truncated NGFR to target geneDepositorInsertICOS (ICOS Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTR-340 pUC19-tNGFR-P2A-IL2RG N
Plasmid#186067PurposeKnockin of truncated NGFR to target geneDepositorInsertIL2RG (IL2RG Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTR-337 pUC19-tNGFR-P2A-CTLA4 N
Plasmid#186054PurposeKnockin of truncated NGFR to target geneDepositorInsertCTLA4 (CTLA4 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTR-342 pUC19-tNGFR-P2A-CD28 N
Plasmid#186056PurposeKnockin of truncated NGFR to target geneDepositorInsertCD28 (CD28 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19 KGA HA5'-mKO2-P2A-Zeo-3'
Plasmid#110408PurposeHomology donor for glutaminase KGA isoform knock-in, mKO2-P2A-ZeoDepositorInsertGLS glutaminase (GLS Human)
UseCRISPRTagsExpressionMutationPromoternoneAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUCHR-mClover-smAID-P2A-CD5Flag2-bglpA
Plasmid#110654PurposeLentiviral vector expressing cell surface Flag-tag and monomeric GFP C-terminaly fused with auxin inducible degronDepositorInsertmClover-AID-P2A-CD5Flag2-bglpA
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUCHR-mClover-smAID-P2A-CD5cmyc2-bglpA
Plasmid#110656PurposeLentiviral vector expressing cell surface c-Myc-tag and monomeric GFP C-terminaly fused with auxin inducible degronDepositorInsertmClover-AID-P2A-CD5cmyc2-bglpA
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
MoClo adapted pUC57-Amp Level 1 position 2
Plasmid#109221PurposeNon binary level 1 position 2 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
MoClo adapted pUC57-Amp Level 1 position 6
Plasmid#109225PurposeNon binary level 1 position 6 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
MoClo adapted pUC57-Amp Level 1 position 4
Plasmid#109223PurposeNon binary level 1 position 4 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
MoClo adapted pUC57-Amp Level 1 position 1
Plasmid#109220PurposeNon binary level 1 position 1 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
MoClo adapted pUC57-Amp Level 1 position 5
Plasmid#109224PurposeNon binary level 1 position 5 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
MoClo adapted pUC57-Amp Level 1 position 7
Plasmid#113044PurposeNon binary level 1 position 7 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only