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Plasmid#160861PurposePJ23110-RBS-AbWS/DGAT-mRuby2-tL3S2P24_KanR_p15ADepositorInsertO-acyltransferase WSD
UseSynthetic BiologyTagsmRuby2ExpressionBacterialMutationPromoterPJ23110Available sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Rluc-let-7-A114-N40
Plasmid#50563PurposeTemplate DNA for in vitro transcription using T7 polymeraseDepositorInsertLuciferase-let7-A114-N40
UseCloning vectorTagsExpressionMutationPromoterlacAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJP_Ctrl10
Plasmid#219672PurposeContains PT3lacO promoter (regulated by blue light and LacI) controlling the expression of mCherry. LacI is constitutively produced by pR promoter.DepositorInsertsmCherry
lacI
UseTagsExpressionMutationPromoterPT3lacO and pRAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJP_Rep00
Plasmid#219677PurposeLight-inducible repressilator (modified from pLPT107). PT3lacO promoter controls the expression of TetR node.DepositorInsertPT3lacO
UseSynthetic BiologyTagsExpressionMutationPromoterPT3lacOAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLNL1143
Plasmid#160869PurposePJ23110-RBS-RsVS-mRuby2-tL3S2P24_KanR_p15ADepositorInsertVinorine synthase
UseSynthetic BiologyTagsmRuby2ExpressionBacterialMutationPromoterPJ23110Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLNL1142
Plasmid#160868PurposePJ23110-RBS-FvSAAT-mRuby2-tL3S2P24_KanR_p15ADepositorInsertAlcohol acyltransferase
UseSynthetic BiologyTagsmRuby2ExpressionBacterialMutationPromoterPJ23110Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLNL1135
Plasmid#160866PurposePJ23110-RBS-CAT-taa-mRuby2-tL3S2P24_KanR_p15ADepositorInsertChloramphenicol Acetyltransferase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPJ23110_BBa_B0029Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLNL1126
Plasmid#160858PurposePJ23110-RBS-FaAAT2-mRuby2-tL3S2P24_KanR_p15ADepositorInsertAlcohol acyl transferase
UseSynthetic BiologyTagsmRuby2ExpressionBacterialMutationPromoterPJ23110Available sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK517
Plasmid#155385PurposePphlF-TA4 in crRNAsDepositorInsertPphlF-TA4 in dCas12a oscillator crRNAs
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ control UTRs
Plasmid#67929PurposeTranslational reporter - control UTRsDepositorInsertsfGFP-NLS-P2A-NLS-mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBoxCDGC-KMet-EGFP
Plasmid#140272PurposeL7Ae-dependent translational repressionDepositorInsertBoxCD
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ Emi1 5' and 3'UTR
Plasmid#67930PurposeTranslational reporter - Emi1 UTRsDepositorInsertsfGFP-NLS-P2A-NLS-mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLNL1133
Plasmid#160864PurposePJ23110-RBS-ScEEB1-mRuby2-tL3S2P24_KanR_p15ADepositorInsertMedium-chain fatty acid ethyl ester synthase
UseSynthetic BiologyTagsmRuby2ExpressionBacterialMutationPromoterPJ23110Available sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVYD
Plasmid#61302PurposeVanillate-inducible gene expression plasmid for Sphingomonas melonis Fr1; destination vector for Gateway cloning, allowing C-terminal fusion of proteins to SYFP2DepositorTypeEmpty backboneUseTagsSYFP2ExpressionBacterialMutationPromoterPV10Available sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
circRAJ31-Toehold_mutant
Plasmid#69930PurposeExpresses a toehold mutant of the circRAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant does not activate cisRAJ31.DepositorInsertplTet-circRAJ31_toeholdmutant
UseSynthetic BiologyTagsExpressionMutation4 bases mutated in toehold of riboregulatorPromoterPltetO1-2Available sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLNL1134
Plasmid#160865PurposePJ23110-RBS-ScEHT1-mRuby2-tL3S2P24_KanR_p15ADepositorInsertMedium-chain fatty acid ethyl ester synthase
UseSynthetic BiologyTagsmRuby2ExpressionBacterialMutationPromoterPJ23110Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDHM1_ARATH
Plasmid#204236PurposeBacterial expression of Arabidopsis mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHM1_ARATH (mMDH1 Mustard Weed)
UseTags6X HisExpressionBacterialMutationDoes done include the first 52 residues that are …PromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDHNP_ARATH
Plasmid#204238PurposeBacterial expression of Arabidopsis chloroplastic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHNP_ARATH (AT5G58330 Mustard Weed)
UseTags6X HisExpressionBacterialMutationDoes not include the chloroplastic transit sequen…PromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pdCas9_CL
Plasmid#166245Purposeregulated dCas9 generatorDepositorInsertP112-sgRNA-term-P104-RBS1-dCas9-B15
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterEc-TTL-P112 and Ec-TTL-P104Available sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLNL1141
Plasmid#160867PurposePJ23110-RBS-PhCFAT-mRuby2-tL3S2P24_KanR_p15ADepositorInsertConiferyl alcohol acyltransferase
UseSynthetic BiologyTagsmRuby2ExpressionBacterialMutationPromoterPJ23110Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only