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Plasmid#74097PurposeBBa_J23100 promoter (iGEM collection)DepositorInsertBba_J23100 constitutive promoter (Sigma70)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterNoneAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCas8
Plasmid#82380PurposedCas9 without a promoter in acsADepositorInsertdCas9
UseTagsExpressionMutationPromoternoneAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas73
Plasmid#82398PurposeΔccm:Km, used to delete ccm operonDepositorInsertccm deletion
UseTagsExpressionMutationPromoternoneAvailable sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-BBa_B0012
Plasmid#72997PurposeBBa_B0012 iGEM terminator (5' TCGA/ 3' TGTT fusion)DepositorInsertBba_B0012 terminator
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-BBa_B0015
Plasmid#72998PurposeBBa_B0015 iGEM terminator (5' TCGA/ 3' TGTT fusion)DepositorInsertBBa_B0015 terminator
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP_BBa_B0034
Plasmid#72980PurposeBba_B0034 iGEM RBS (5' GTAC/3' CATA fusion)DepositorInsertBba_B0034 RBS
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
TetOn-mCherry-eGFP-RAMP4
Plasmid#109014PurposeTet-On construct to induce expression of tandem eGFP-mCherry tagged RAMP4 (ER marker)DepositorInsertRAMP4 (SERP1 Human)
UseLentiviralTagsmCherry-eGFPExpressionMammalianMutationPromoterCMV promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt2
Plasmid#86593PurposeExpresses eGFP-tagged human Akt2(WT)DepositorInsertAkt2 (AKT2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBPhsFlp2::PEST
Plasmid#63171PurposeDrosophila codon-optimized Flp2::mODC-PEST fusion gene driven from Drosophila Hsp70Bb Heat Shock PromoterDepositorInserthsFlp2::PEST
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJ151-HDR
Plasmid#113631PurposeContains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.DepositorInsertsLeft homology arm
Puromycin resistance gene
Venus fluorescence gene
Thymidine kinase
Right homology arm
UseCre/Lox; Homology recombination selection vectorTagsExpressionBacterial and MammalianMutationPromoterEF1a promoter, EF1a promoter (T2A cleaving sequen…Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pK18sB
Plasmid#177838PurposePlasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size and lacking oriT required for conjugation.DepositorTypeEmpty backboneUseBacterial gene replacementTagsExpressionMutationPromoterAvailable sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
Stx1a-12aa-mCer
Plasmid#53236PurposeFluorescent probe: Syntaxin 1A, C-terminally-tagged with monomeric Cerulean, connected with a 12 amino acid linker. Expressed in mammalian cells, used with SNAP25B-12aa-mCit to measure FRET & dispers.DepositorInsertSyntaxin 1A (Stx1a Rat)
UseTags12aa-linker and mCeruleanExpressionMammalianMutationPromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEF1-alpha-V
Plasmid#27290DepositorInsertintegrin alpha V (ITGAV Human)
UseTagsExpressionMammalianMutationIsoform 2PromoterAvailable sinceMarch 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCI-SEP_NR2B
Plasmid#23998DepositorInsertNR2B (Grin2b Rat)
UseTagsNR2B signal sequence (aa1-31) and SEP (supereclip…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5
Plasmid#63168PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-V5 reporterDepositorInsert"spaghetti monster GFP-V5"
UseTagsN-myristoylationExpressionInsectMutationPromoterAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCI-SEP_NR2A
Plasmid#23997DepositorInsertNR2A (Grin2a Rat)
UseTagsSEPExpressionMammalianMutationPromoterAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO ChETA-EYFP
Plasmid#26968PurposeAAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by EF1a promoter for optogenetic activationDepositorHas ServiceAAV1, AAV5, and AAV9InserthChR2(E123T/H134R)-EYFP
UseAAVTagsEYFPExpressionMammalianMutationE123T, H134RPromoterEF1alphaAvailable sinceJan. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
Adeno Cas9
Plasmid#64072PurposeAdenoviral vector for the expression of the Cas9DepositorTypeEmpty backboneUseAdenoviralTagsFLAGExpressionMutationPromoterU6 and CBhAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJFRC201-10XUAS-FRT>STOP>FRT-myr::smGFP-HA
Plasmid#63166PurposeGAL4-responsive UAS "flp-On" spaghetti monster GFP-HA reporterDepositorInsert"spaghetti monster GFP-HA"
UseTagsN-myristoylationExpressionInsectMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-C1C2-TS-EYFP
Plasmid#35520DepositorInsertChR1-ChR2 Chimera
UseLentiviralTagsTrafficking signal and EYFPExpressionMammalianMutationPromoterCaMKIIaAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only