Addgene

We narrowed to 51,996 results for: cloning

Showing: 901 - 920 of 51996 results

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pNC-Cam1304-35S

Plasmid

#193384

Purpose

Nimble cloning-compatible vector for plant ectopic expression

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pNC-Amp-GFP-C

Plasmid

#193417

Purpose

Nimble cloning-compatible vector for protein subcellular localization in plants

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pNC-Amp-35S

Plasmid

#193398

Purpose

Nimble cloning-compatible vector for plant ectopic expression

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pNC-Amp-GFP-N

Plasmid

#193416

Purpose

Nimble cloning-compatible vector for protein subcellular localization in plants

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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C232-E08: attB2r-mTFP1-attB3

Plasmid

#162904

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal mTFP1 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Teal fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E18: attB4-polyhedrinp>-attB5

Plasmid

#162977

Purpose

Gateway attB4/attB5 entry clone for baculovirus expression using the AcMNPV polyhedrin promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

AcMNPV polyhedrin promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E21: attB4-FerHp>-attB5

Plasmid

#162978

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using the human EF1alpha promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human Ferritin heavy chain promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E38: attB4-T7p>-attB1r

Plasmid

#162929

Purpose

Gateway attB4/attB1r entry clone for bacterial expression using the bacteriophage T7 promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

bacteriophage T7 promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E26: attB4-T7p>-attB5

Plasmid

#162982

Purpose

Gateway attB4/attB1r entry clone for bacterial expression using the bacteriophage T7 promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

bacteriophage T7 promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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C512-E01: attB5r-meGFP-nostop-attB1r

Plasmid

#162940

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal eGFP fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric enhanced Green fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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C511-E15: attB5r-ffluc2-nostop-attB1r

Plasmid

#162939

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal Nanoluciferase fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Firefly luciferase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E40: attB4-EFSp>-attB1r

Plasmid

#162928

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the short EF1 promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

short form of human EF1 alpha promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E34: attB4-mCMVp>-attB1r

Plasmid

#162926

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the minimal CMV promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

minimal CMV IE1 promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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C238-E07: attB2r-SV40polyA-attB3

Plasmid

#162917

Purpose

Gateway attB2r/attB3 entry clone containing a polyA sequence for transcription termination

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

SV40 polyadenylation sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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C234-E24: attB2r-Strep2-attB3

Plasmid

#162913

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal Strep2 tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Strep2 epitope tag

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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C232-E05: attB2r-meGFP-attB3

Plasmid

#162902

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal eGFP fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric enhanced Green fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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C231-E02: attB2r-NanoLuc-attB3

Plasmid

#162901

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal NanoLuc fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Nanoluciferase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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C232-E06: attB2r-mCherry-attB3

Plasmid

#162903

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal mCherry fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Cherry fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E25: attB4-mPol2p>-attB5

Plasmid

#162981

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using the murine RNA polymerase 2 promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

murine RNA polymerase 2 promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E04: attB4-CMV51p>-attB5

Plasmid

#162973

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using an optimized human CMV promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

optimized human CMV promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge