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Plasmid#67125PurposeStandard for cell-free expression benchmarking.DepositorInsertWWP1 (WWP1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
ALIX-mNeonGreen
Plasmid#191191PurposeFull-length ALIX with C-terminal mNeonGreen tag.DepositorInsertALIX (PDCD6IP Human, Synthetic)
UseTags6xHIS and mNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
p6592 MSCV-IP N-HAonly WWP2
Plasmid#34905DepositorInsertWWP2 (WWP2 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterMSCV LTRAvailable sinceFeb. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pY2F
Plasmid#53285PurposeContains an Arabidopsis thaliana lcyE cDNA and produces an active lycopene epsilon-cyclase enzyme in E. coli. Use with pAC-BETA-At or pAC-BETAipi-At to produce alpha-carotene in E. coli.DepositorInsertlcyE (LUT2 Mustard Weed)
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
ALIX-His(1-868, P801G)
Plasmid#186808PurposeCodon-optimized bacterial expression plasmid. Expresses ALIX-His(1-868, P801G).DepositorInsertALIX-His (1-868, P801G) (PDCD6IP Human)
UseTagsHis6ExpressionBacterialMutationProline 801 to GlycinePromoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAtCHYb1Δ129N
Plasmid#53367PurposeContains a truncated Arabidopsis beta-carotene hydroxylase cDNA, the product of which converts beta-carotene primarily to monocyclic beta-cryptoxanthin in E. coli. Use with pAC-BETA or pAC-BETAipi.DepositorInsertchyB1 (BETA-OHASE 1 Mustard Weed)
UseTagsExpressionBacterialMutationDeleted codons for the first 129 amino acidsPromoterTrcAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTRdeltaGFPmirGE-DroshaT1.5.5
Plasmid#61084PurposepENTR plasmid containing a mutated GFP followed by 3 miRNA haipins: recommended as backbone.DepositorInsertdeltaGFP
UseRNAiTagsExpressionMutationPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PDCD6IP_BRO1
Plasmid#109994PurposeProtein expression and purification of PDCD6IP_BRO1DepositorInsertPDCD6IP_BRO1 (PDCD6IP Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC-CANTHipi
Plasmid#53301PurposeContains crtE, idi, crtI, crtY, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and a crtO cDNA of Haematococcus pluvialis fused to a Trc promoter. Produces canthaxanthin in E. coli.DepositorInsertcrtO
UseLow copy number bacterial cloning vectorTags6HisExpressionMutationPromoterTrcAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
ALIX(P801G)-Strep
Plasmid#180024PurposeCodon-optimized bacterial expression plasmid. Expresses ALIX(1-868, P801G)-Strep. P801G mutation facilitates expression in E. coli.DepositorInsertALIX(1-868, P801G)-Strep (PDCD6IP Human)
UseTagsStrep tag (WSHPQFEK)ExpressionBacterialMutationP801GPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_PRDX6-Halo
Plasmid#175338PurposeProduction of HaloTag fusion proteinDepositorInsertPRDX6 (PRDX6 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pet151D-TOPO ALIX Bro1-V (1-702)
Plasmid#80642PurposeResidues 1-702 of ALIX; Bacterial Expression Vector; TEV cleavable N-term His-tag; Sundquist Lab Internal ID: WISP10-648DepositorInsertALIX Bro1-V (PDCD6IP Human)
UseTagsHis TagExpressionBacterialMutationResidues 1-702PromoterAvailable sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pet16b ALIX Bro1 (1-359)
Plasmid#80641PurposeResidues 1-359 of ALIX; Bacterial Expression Vector; N-term His-tag; Sundquist Lab Internal ID: WISP11-428DepositorInsertALIX Bro1, residues 1-359 (PDCD6IP Human)
UseTagsHis TagExpressionBacterialMutationResidues 1-359PromoterT7Available sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
SFFV-CSRNP1-Brd 412
Plasmid#219334PurposeTranscription factor CSRNP1 with a specific barcode assigned.DepositorInsertCSRNP1 (CSRNP1 Human)
UseLentiviralTagsExpressionMutationCodon optimizedPromoterThe spleen focus-forming virus (SFFV) promoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-Tev-ALIX-V (348-702)
Plasmid#189819PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His-Tev-ALIX-V (348-702).DepositorInsertGB1-His-Tev-ALIX-V (348-702) (PDCD6IP Human)
UseTagsGB1-His-TevExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM33_PDCD6IP_ALIX V-domain
Plasmid#178483PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertPDCD6IP_ALIX V-domain (PDCD6IP Human, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCINeo-myc-Itch/2859
Plasmid#11427DepositorInsertItch (Itch Mouse)
UseTagsmycExpressionMammalianMutationn/aPromoterAvailable sinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-OTULIN (full-length, aa 1-352)
Plasmid#61464PurposeExpresses human OTULIN (full-length) in E. coli.DepositorInsertOTULIN (OTULIN Human)
UseTagsHis6-3CExpressionBacterialMutationPromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-KG-Itch/2793
Plasmid#11434DepositorInsertItch (Itch Mouse)
UseTagsGSTExpressionBacterialMutationn/aPromoterAvailable sinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only