Addgene

We narrowed to 3,013 results for: actin

Showing: 901 - 920 of 3013 results

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pcDNA3.1-HA-ARHGAP29

Plasmid

#104154

Purpose

mammalian expression of HA tagged ARHGAP29

Depositor

Marius Sudol

Article

Qiao et al Cell Rep. 2017 May 23;19(8):1495-1502. d

Insert

ARHGAP29 (ARHGAP29 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-ACTB_sgRNA

Plasmid

#183885

Purpose

pX459V2.0-HypaCas9 plasmid with ACTB sgRNA for N-terminal tagging of beta-actin in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

ACTB sgRNA spacer (ACTB Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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His-MBP_G4G6 in pRSF

Plasmid

#188454

Purpose

Express in E. coli human gelsolin segments 4 through 6 with N-terminal 6xHis and MBP tags.

Depositor

Roberto Dominguez

Article

Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

Insert

G4G6 (GSN Human)

Use

Tags

6xHis-MBP

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLXSN-H-Ras_N17

Plasmid

#39537

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS N17 (HRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

N17

Promoter

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
phage - ubc - nls - 2xmcp - egfp - BirA*

Plasmid

#131132

Purpose

BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.

Depositor

Ralf Jansen

Article

Mukherjee et al Proc Natl Acad Sci U S A. 2019 Jun 25;11

Insert

NLS-HA-2xMCP-EGFP-BirA*

Use

Lentiviral

Tags

NLS, HA

Expression

Mammalian

Mutation

BirA* = BIrA >R118G

Promoter

human ubiquitin C (UBC)

Available since

Aug. 30, 2019

Availability

Academic Institutions and Nonprofits only

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EHD2-T72A-mEGFP

Plasmid

#45934

Purpose

dominant-negative EHD2 mutant

Depositor

Ari Helenius

Article

Stoeber et al EMBO J. 2012 May 16;31(10):2350-64. doi:

Insert

Eps-15 homology domain-containing protein2 (EHD2 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

T 72 changed to A (T72A)

Promoter

CMV

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pET28b_RanT24N

Plasmid

#186271

Purpose

Expression vector of His-RanT24N in E.coli

Depositor

Masahito Ikawa

Article

Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

GTP-binding nuclear protein Ran (Ran Mouse)

Use

Tags

His tagged and His-tagged at N terminal

Expression

Bacterial

Mutation

Changed T24 into N

Promoter

T7 promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pB80-KIF1A(1-383)-GFP-SSPB(milli)

Plasmid

#174626

Purpose

Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(milli)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-383)-GFP-SSPB(milli) (Kif1a Mouse, Synthetic)

Use

Tags

GFP-SSPB(milli)

Expression

Mammalian

Mutation

mmKIF1A(aa1-383): Pro202Ala; EGFP: Met1Del; SSPB:…

Promoter

Chicken beta-actin

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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S142D drebrin-YFP

Plasmid

#58336

Purpose

mammalian expression of human drebrin-YFP phospho-mimetic mutant

Depositor

Phillip Gordon-Weeks

Article

Worth et al J Cell Biol. 2013 Sep 2;202(5):793-806.

Insert

drebrin (DBN1 Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

S142D, phospho-mimetic mutant

Promoter

CMV

Available since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only
S142A drebrin-YFP

Plasmid

#58335

Purpose

mammalian expression of human drebrin-YFP phospho-dead mutant

Depositor

Phillip Gordon-Weeks

Article

Worth et al J Cell Biol. 2013 Sep 2;202(5):793-806.

Insert

drebrin (DBN1 Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

S142A, phospho-dead mutant

Promoter

CMV

Available since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-EPLIN alpha delta LIM

Plasmid

#40933

Depositor

Scott Maul, Elizabeth Luna

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN alpha (LIMA1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

LIM domain deleted- amino acids 221-295

Promoter

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-EPLIN alpha delta C-term

Plasmid

#40930

Depositor

Scott Maul, Elizabeth Luna

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN alpha (LIMA1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 296-600

Promoter

Available since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only
M3-PSCFP2

Plasmid

#114185

Purpose

studying clustering of M3 receptors by superresolution microscopy (PALM)

Depositor

Aleksandra Radenovic

Article

Scarselli et al J Biol Chem. 2012 May 11;287(20):16768-8

Insert

Muscarin acetylcholine receptor 3 (CHRM3 Human)

Use

Tags

3xHA and PS-CFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLXSN-H-Ras_V12

Plasmid

#39516

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 (HRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

G12V

Promoter

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
EHD2-T72A-mCherry

Plasmid

#45935

Purpose

dominant-negative EHD2 mutant

Depositor

Ari Helenius

Article

Stoeber et al EMBO J. 2012 May 16;31(10):2350-64. doi:

Insert

Eps-15 homology domain-containing protein2 (EHD2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

T 72 changed to A (T72A)

Promoter

CMV

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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EGFP-SPN_ARR wt (Shank3)

Plasmid

#175254

Purpose

SPN-ARR fragment in EGFP backbone for mammalian expression

Depositor

Johanna Ivaska

Article

Salomaa et al Curr Biol. 2021 Sep 29. pii: S0960-9822(

Insert

SPN-ARR fragment from SHANK3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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human TLNRD1 pET151

Plasmid

#159384

Purpose

Expresses human TLNRD1 in bacterial cells

Depositor

Ben Goult

Article

Cowell et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

Talin Rod Domain Containing Protein 1 (TLNRD1 Human)

Use

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pB80-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11

Plasmid

#174645

Purpose

Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)-P2A-ILID-mCherry-RAB11

Use

Tags

SSPB(micro)-Venus and iLID-mCherry

Expression

Mammalian

Mutation

SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…

Promoter

Chicken beta-actin

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP TEM4 R130D

Plasmid

#58895

Purpose

Expresses GFP Tagged full length TEM4 protein with R130D mutation. Please see notes below for information regarding the L722F mutation in TEM4.

Depositor

Channing Der

Article

Mitin et al PLoS One. 2012;7(7):e41876. doi: 10.1371

Insert

TEM4 (ARHGEF17 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

R130D, L722F

Promoter

CMV

Available since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP TEM4 DEL 125-135

Plasmid

#58896

Purpose

Expresses GFP tagged full length TEM4 protein with amino acids 125 - 135 deleted

Depositor

Channing Der

Article

Mitin et al PLoS One. 2012;7(7):e41876. doi: 10.1371

Insert

TEM4 (ARHGEF17 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deleted aa's 125-135

Promoter

CMV

Available since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only