Addgene

We narrowed to 28,087 results for: eng

Showing: 9161 - 9180 of 28087 results

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AAV-oChEF-citrine

Plasmid

#50975

Purpose

AAV transfer vector with mammalian codon-optimized ChEF-citrine between AAV2 ITR

Depositor

Roger Tsien

Article

Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

Insert

ChEF-citrine

Use

AAV

Tags

citrine

Expression

Mutation

ChEF gene is mammalian codon optimized

Promoter

human synapsin promoter

Available since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPglu-2uDelta13

Plasmid

#13561

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16F D152A Delta13

Use

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

F16A, D152A in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pZEG

Plasmid

#129112

Purpose

Z. rouxii episomal vector containing G418 resistance

Depositor

Paolo Giudici

Article

Bizzarri et al Yeast. 2019 Dec;36(12):711-722. doi: 10.

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pJEC578

Plasmid

#174366

Purpose

dCas9 for modular CRISPRa. SYNZIP-dCas9. Expresses dCas9 with a SYNZIP18 domain fused to the N terminus.

Depositor

James Chappell

Article

Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

Insert

dCas9 fused to SYNZIP18

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23150

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 S100A1

Plasmid

#67048

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

S100A1 (S100A1 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
mEos2-VASP-5

Plasmid

#57433

Purpose

Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

VASP

Use

Tags

mEos2

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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IFP1.4-C1

Plasmid

#54782

Purpose

Localization: C1 Cloning Vector, Excitation: 684, Emission: 708

Depositor

Michael Davidson, Roger Tsien

Article

Shu et al Science. 2009 May 8;324(5928):804-7. doi

Type

Empty backbone

Use

Tags

IFP1.4

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pCfB4586

Plasmid

#106124

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked Ura3 marker, integration into Yarrowia lipolytica chromosomal location IntB, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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mPapaya-Lifeact-7

Plasmid

#54540

Purpose

Localization: Actin, Excitation: 530, Emission: 541

Depositor

Michael Davidson, Robert Campbell

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Type

Empty backbone

Use

Tags

mPapaya-Lifeact

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only
pPI464

Plasmid

#113216

Purpose

generic knockin vector for C-terminal tagging with mScarlet

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Generic knockin vector for c-terminal tagging wit…

Tags

mScarlet

Expression

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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RAB14 shRNA

Plasmid

#61496

Purpose

to knockdown endogenous RAB14 expression levels

Depositor

Curt Civin

Article

Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

Insert

RAB14 (RAB14 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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TAL3512

Plasmid

#47231

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-pcdh10a-left (pcdh10a Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only
pET-s34T

Plasmid

#28256

Depositor

Yawen Bai

Article

Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

Insert

single chain H3-H4 with Thrombin site

Use

Tags

His

Expression

Bacterial

Mutation

A thrombin cutting site between H3 and H4

Promoter

Available since

July 11, 2011

Availability

Academic Institutions and Nonprofits only
CAG::PSAMQ79G,Q139G:5HT3HC-IRES-GFP

Plasmid

#32474

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAMQ79G,Q139G:5HT3 HC

Use

Tags

IRES-EGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 10, 2012

Availability

Academic Institutions and Nonprofits only

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pCfB8622

Plasmid

#126912

Purpose

Plasmid containing gRNA expression cassette targeting ADE2 in Saccharomyces cerevisiae

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

ADE2 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)

Plasmid

#101105

Purpose

Encodes dCas9(C) (without VP64) fused to VEGFR1

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

VEGFR1-dCas9(C)

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pZLSLD.R

Plasmid

#51511

Purpose

Single component LSL vector. Contains 2x35S:Zif268:FokI outside of the replicon borders. Within the replicon is the us:NPTII template.

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

Zif268:FokI
us:NPTII repair template

Use

Plant t-dna plasmid

Tags

NLS

Expression

Mutation

Promoter

2x35S

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 SREBF2

Plasmid

#67129

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

SREBF2 (SREBF2 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pBS-67Sma

Plasmid

#49342

Purpose

Chlamy Cp transformation for transgene integration via double-homologous recombination into the rbcL-psaB intergenic region

Depositor

Anastasios Melis

Article

Chen et al Plant Biotechnol J. 2013 Sep;11(7):818-2

Insert

EcoRI 5.8 kB

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pRoDonor

Plasmid

#178773

Purpose

Expression of RoCAST left end, cam, RoCAST right end from Rippkaea orientalis

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

RoCAST left end, cam, RoCAST right end from Rippkaea orientalis

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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