Addgene

We narrowed to 11,164 results for: cel.2

Showing: 9161 - 9180 of 11164 results

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CD200-Cd4d3+4-COMP-blac-3xFLAG-6his

Plasmid

#71471

Purpose

A positive control receptor prey (rat CD200) EXPRESs plasmid for The Basic AVEXIS kit

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Insert

CD200 (Cd200 Rat)

Use

Tags

6xHis tag, Cd4d3+4, beta-lactamase, and triple FL…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmeIF4G-trunc-V5His6_G

Plasmid

#146392

Purpose

Insect Expression of DmeIF4G-trunc. *Note: this plasmid does not contain an HA-tag as the name implies.

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Sep 21. ():.

Insert

DmeIF4G-trunc (eIF4G1 Fly)

Use

Tags

Expression

Insect

Mutation

322-1666 truncated version of elF4G sequence with…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO GFP hBub1 K821R

Plasmid

#59813

Purpose

Allows the integration of GFP hBub1 K821R in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Perera et al J Cell Sci. 2010 Mar 1;123(Pt 5):653-9.

Insert

Bub1 (BUB1 Human)

Use

Tags

EmGFP

Expression

Mammalian

Mutation

K821R

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO GFP hBub1 WT

Plasmid

#59812

Purpose

Allows the integration of GFP hBub1 in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Perera et al J Cell Sci. 2010 Mar 1;123(Pt 5):653-9.

Insert

Bub1 (BUB1 Human)

Use

Tags

EmGFP

Expression

Mammalian

Mutation

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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p55-H2B-Dendra2

Plasmid

#80609

Purpose

Encodes H2B-Dendra2-fusion protein under the CAG promoter. Linearize using Asc1 and Not1 for mouse embryo injection.

Depositor

Periklis Pantazis

Article

Mohr et al Nat Protoc. 2016 Dec;11(12):2419-2431. d

Insert

H2B-Dendra2 (H2BC21 Human, Synthetic)

Use

Tags

Dendra2

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-FNTA-FLAG

Plasmid

#86858

Purpose

To express FNTA with a FLAG tag at C terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

FNTA (FNTA Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

The sequence of the insert is isoform 2, which di…

Promoter

CMV

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-GFP-ATL2-deltaTM

Plasmid

#109021

Purpose

stable lentiviral expression of cDNA

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL2-deltaTM (ATL2 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

truncated form without C-terminal ER transmembran…

Promoter

EIF-1a promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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3611 pcDNA3 Bim

Plasmid

#8804

Depositor

Stanley Korsmeyer

Article

Harada et al Proc Natl Acad Sci U S A 2004 Oct 26;101

Insert

Bim (Bcl2l11 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
KHBD00642

Plasmid

#39706

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG5748 (Hsf Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only
sgp27#1

Plasmid

#102759

Purpose

Expresses sgRNA targeting mouse p27

Depositor

Jin Chen

Article

Song et al Oncogene. 2017 Jun 5. doi: 10.1038/onc.2

Insert

p27 (Cdkn1b Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 1, 2017

Availability

Academic Institutions and Nonprofits only

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B9L-BirA*

Plasmid

#109146

Purpose

To generate stable cell lines expressing B9L-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cells

Depositor

Mariann Bienz

Article

van Tienen et al Elife. 2017 Mar 15;6. doi: 10.7554/eLife

Insert

B9L (Bcl9l Mouse)

Use

Tags

BirA(R118G) and Flag

Expression

Mammalian

Mutation

Promoter

CMV/TetO2

Available since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgATL2-1

Plasmid

#109008

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL2-sgRNA #1 (ATL2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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KHBD00551

Plasmid

#39646

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG1849 (run Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
3625 pSP72 Bim (genomic)

Plasmid

#8805

Depositor

Stanley Korsmeyer

Article

Harada et al Proc Natl Acad Sci U S A 2004 Oct 26;101

Insert

Bim genomic (Bcl2l11 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pDK366

Plasmid

#134196

Purpose

Retroviral plasmid with SKAP insert

Depositor

Iain Cheeseman

Article

Kern et al J Cell Biol. 2016 May 9;213(3):315-28. d

Insert

5xRD SKAP Alternate Start- LAP (with G5 hardeneded) (KNSTRN Human)

Use

Retroviral

Tags

LAP (GFP)

Expression

Mammalian

Mutation

5xRD, Alternate Start, G5 hardeneded

Promoter

Available since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-Atoh1 del3

Plasmid

#96913

Purpose

Murine Atoh1 with amino acids 214-305 removed

Depositor

Albert Edge

Article

Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

Insert

Atoh1 (Atoh1 Mouse)

Use

Tags

2xFLAG

Expression

Mammalian

Mutation

Deletion of amino acids 214-305

Promoter

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-Atoh1 del4

Plasmid

#96914

Purpose

Murine Atoh1 with amino acids 306-347 removed

Depositor

Albert Edge

Article

Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

Insert

Atoh1 (Atoh1 Mouse)

Use

Tags

2xFLAG

Expression

Mammalian

Mutation

Deletion of amino acids 306-347

Promoter

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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KHBD00701

Plasmid

#39737

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG6464 (salm Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pCI neo-hEST2

Plasmid

#1781

Depositor

Bob Weinberg

Article

Meyerson et al Cell 1997 Aug 22;90(4):785-95.

Insert

hTERT (TERT Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

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pCDH-SIRT2-Flag

Plasmid

#102624

Purpose

Expresses full length SIRT2 isoform 1 in mammalian cells

Depositor

Hening Lin

Article

Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

Insert

SIRT2 (SIRT2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2018

Availability

Academic Institutions and Nonprofits only

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