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Plasmid#138514PurposeP3gDepositorInsertT7-RNAP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJH74_pCMV_hyCal7
Plasmid#160419PurposeCMV promoter driving HyCal component (HyNaC7)DepositorInsertpEZY3 CMV-hyCal7
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH75_pCMV_hyCal9
Plasmid#160420PurposeCMV promoter driving HyCal component (HyNaC9)DepositorInsertpEZY3 CMV-hyCal9
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30-T7-His-sfGFP
Plasmid#180755PurposepET30 vector missing lacO with sfGFP-S-tag inserted between the Bgl II and T7 terminatorDepositorInsertsuperfolder green fluorescent protein
UseTagsstrep tagExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp-GST
Plasmid#134592PurposeLow Phosphate inducible GSTDepositorInsertGlutathione S-transferase
UseTagsnoneExpressionBacterialMutationPromoteryibDAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDM1080
Plasmid#108980PurposeKnockout cassette NourseothricinDepositorInsertKnockout cassette Nourseothricin
UseCre/Lox; Dictyostelium discoideum knockout vectorTagsExpressionMutationPromotercoaAAvailable sinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJZC523
Plasmid#62320PurposesgRNA for yeast cellsDepositorInsertsgRNA
UseTagsExpressionYeastMutationPromoterSNR52Available sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Talin-N-22
Plasmid#57673PurposeLocalization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertTalin
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX Smad3 LC
Plasmid#12633DepositorInsertSmad3 LC (SMAD3 Human)
UseTagsGSTExpressionBacterialMutationLC segment of Smad3 (aa 146-425)PromoterAvailable sinceOct. 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
FnR9-10
Plasmid#182567PurposeHuman fibronectin fragment, type-III repeats 9-10, R1379A mutantDepositorInsertHuman fibronectin fragment, type-III repeats 9-10
UseTagsHis and TEV cleavage site after His tagExpressionBacterialMutationR1379APromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pI2-MTU-DO-HIS
Plasmid#160016PurposeIntegrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Histidine selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7_circRAJ31_C873U
Plasmid#69932PurposeExpresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant is deficient in circularisation. Under control of a T7 promoter.DepositorInsertpT7_circRAJ31_C873U
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPglu-170nDelta13
Plasmid#13562DepositorInsertFLIPglu F16F Delta13
UseTagsECFP and EYFPExpressionBacterialMutationWT binding protein (no F16A)PromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pJZC583
Plasmid#62314PurposesgRNA with 2x MS2 for yeast cellsDepositorInsertsgRNA + 2x MS2 binding module
UseTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
mEos2-Talin-N-22
Plasmid#57420PurposeLocalization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertTalin
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-Flag-YAP-4SA(S127)
Plasmid#33082DepositorInsertYAP (YAP1 Human)
UseTagsFlagExpressionMammalianMutationS61A, S109A, S164A, S381APromoterCMVAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
sv272-HisMBP-TZAPZNF9-11
Plasmid#87185Purposebacterial expression of HisMBP-TZAPZNF9-11DepositorInsertTZAP (ZBTB48 Human)
UseTagsHisMBPExpressionBacterialMutationPromoterT7Available sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 USF2
Plasmid#67069PurposeStandard for cell-free expression benchmarking.DepositorInsertUSF2 (USF2 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Talin-18
Plasmid#57672PurposeLocalization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertTalin
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits