Addgene

We narrowed to 18,521 results for: omp

Showing: 9021 - 9040 of 18521 results

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pcDNA4/TO-myc NES-mCE NLS-Flag

Plasmid

#82468

Purpose

Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

Myc-NES-mCE-Flag (Rngtt HIV, Mouse)

Use

Tags

Flag and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCDH_CMV_HMGA_linker_mKate2

Plasmid

#167335

Purpose

Expresses mKate2 fused to TFAM HMGA+linker mutant in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Mutation

Truncation mutant containing MTS (1-42) as well a…

Promoter

CMV

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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px330 CBP gRNA

Plasmid

#165587

Purpose

insertin of CBP degrom

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

CBP (Crebbp Mouse)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AMPKsub-YPet-Kras

Plasmid

#84638

Purpose

Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

AMPKsub-YPet-Kras

Use

Tags

6xHis, C-terminal targeting sequence from Kras, T…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV10/3xflag-Clock L57E

Plasmid

#47337

Purpose

Mutation used for co-IPs

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Use

Tags

3x Flag

Expression

Mammalian

Mutation

L57E

Promoter

CMV

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pCMV10/3xflag-Clock L74E

Plasmid

#47338

Purpose

Mutation used for co-IPs

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Use

Tags

3x Flag

Expression

Mammalian

Mutation

L74E

Promoter

CMV

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pHH0103_NEDD9-1/1

Plasmid

#91498

Purpose

Protein expression and purification of human SH3 domain construct NEDD9-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NEDD9-1/1 (NEDD9 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAAVsc.U6-sgGTTG.CMV/CB-Gluc

Plasmid

#121512

Purpose

Expresses sgRNA targeting the intron in the MADM alleles (GT and TG).

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgGTTG

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pSR322

Plasmid

#125116

Purpose

Expression of uhpA(1-123aa)-ydfI(126-213aa), narL131 chimera under PLtetO-1, output Promoter PydfJ115

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

uhpA(REC)-ydfI(DBD)131
sfgfp
tetR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX Tenc1-SH2

Plasmid

#46524

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Tenc1 (TNS2 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1114-1264

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Socs5-SH2

Plasmid

#46508

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Socs5 (SOCS5 Human)

Use

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 378-481

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pDECKO-mCherry TFRC_B

Plasmid

#78544

Purpose

Experimental plasmid

Depositor

Rory Johnson, Roderic Guigo

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 and U6 (for expressing sgRNA)

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX Grap-SH2

Plasmid

#46434

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grap (GRAP Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 51-160

Promoter

Tac

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pHH0103_SRC-1/1-IS1/2

Plasmid

#91349

Purpose

Protein expression and purification of human SH3 domain construct SRC-1/1-IS1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SRC-1/1-IS1/2 (SRC Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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PVT1-Tol2

Plasmid

#113381

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

PVT1-enhancer (PVT1 Human)

Use

Tol2 reporter

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only
pBABEhygro-Flag-ZO-2-1st-PDZ-mut

Plasmid

#27474

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

K38E, F44H, I46E and V48E

Promoter

Available since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Ywhag-3'UTR-MRE-3 MUT

Plasmid

#61792

Purpose

Luciferase reporter containing the 3'UTR of mouse Ywhag with MRE-3 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Ywhag 3'UTR with miR-200c MRE-3 mutated

Use

Luciferase

Tags

Expression

Mutation

miR-200c MRE-3 mutated

Promoter

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pLacIBD::SDRX (GB0673)

Plasmid

#68183

Purpose

Provides the LacI Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

LacI Binding Domain fused to SRDX Transcriptional Repressor

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pHH0103_NOSTRIN-1/1-SV1/2

Plasmid

#91323

Purpose

Protein expression and purification of human SH3 domain construct NOSTRIN-1/1-SV1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NOSTRIN-1/1-SV1/2 (NOSTRIN Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3KBP1-1/3

Plasmid

#91555

Purpose

Protein expression and purification of human SH3 domain construct SH3KBP1-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3KBP1-1/3 (SH3KBP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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