Addgene

We narrowed to 18,521 results for: omp

Showing: 9001 - 9020 of 18521 results

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TDP-43_CTD_M337V

Plasmid

#98674

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with M337V

Depositor

Nicolas Fawzi

Article

Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

Insert

TDP-43 (TARDBP Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

M337V

Promoter

Available since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pSR06

Plasmid

#69152

Purpose

read-outloxN mCherry to GFP switch for integration on ttTi5605, Mos Chr II

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Inserts

mCherry
eGFP

Use

Cre/Lox; Mossci

Tags

Expression

Worm

Mutation

codon-optimzed index 1.0 and codon-optimzed index…

Promoter

rps-27

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pHH0103_PSTPIP1-1/1

Plasmid

#91453

Purpose

Protein expression and purification of human SH3 domain construct PSTPIP1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PSTPIP1-1/1 (PSTPIP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pFL His-SDS22

Plasmid

#113512

Purpose

Expression of His-SDS22 in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

SDS22 (PPP1R7 Human)

Use

Tags

6*His

Expression

Insect

Mutation

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pUASp-FMW-attB-AthHen1

Plasmid

#104957

Purpose

Expression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila germline tissues using Gal4 drivers

Depositor

Stefan Ameres

Article

Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

Insert

Hen1 (HEN1 Mustard Weed)

Use

Tags

FLAG Myc

Expression

Insect

Mutation

Promoter

pTrasposase promoter

Available since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX BRDG1-SH2

Plasmid

#46410

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

BRDG1 (STAP1 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 167-283

Promoter

Tac

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HRPT2 L64P

Plasmid

#11052

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

HRPT2 L64P (CDC73 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

L64P

Promoter

Available since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
pGEX Vav3-SH2

Plasmid

#46530

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Vav3 (Vav3 Mouse)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 660-774

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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phumanCas9 (GB0575)

Plasmid

#68206

Purpose

Provides the human codon optimized CDS of Cas9 protein as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Cas9 coding region (human codon optimised)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed; human codon optimis…

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ERKsub(TA)DD-Venus-NES

Plasmid

#84631

Purpose

Encodes negative-control C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

ERKsub(TA)DD-Venus-NES

Use

Tags

6xHis, Nuclear export signal (NES), T7 tag (gene …

Expression

Mammalian

Mutation

Mutated target Thr residue to Ala.

Promoter

CMV

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX Shb-SH2

Plasmid

#46485

Depositor

Bruce Mayer, Michael Welsh

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Shb (SHB Human)

Use

Tags

GST, HA, and Thrombin site

Expression

Bacterial

Mutation

contains amino acids 401-508

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pDESTmycEIF2C1

Plasmid

#19871

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Oct 31. ():.

Insert

eukaryotic translation initiation factor 2C, 1 (AGO1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-THAP11-rescue

Plasmid

#37001

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Insert

THAP11 (THAP11 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Mutations were generated at codons 273-275 conver…

Promoter

Available since

May 21, 2012

Availability

Academic Institutions and Nonprofits only
PD-L1-miSFIT-6-C, 16-C

Plasmid

#124689

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-L1 (CD274 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

minimal CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_PIK3R1-1/1

Plasmid

#91274

Purpose

Protein expression and purification of human SH3 domain construct PIK3R1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PIK3R1-1/1 (PIK3R1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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px330 CTCF gRNA

Plasmid

#165588

Purpose

Insertion of CTCF degon

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

CTCF (CTCF Mouse)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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SPOP-V5 (BTB)

Plasmid

#169721

Purpose

Mammalian Expression of mouse SPOP-BTB (L186D, L190D, L193D, I217K)

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

SPOP (Spop Mouse)

Use

Tags

V5

Expression

Mammalian

Mutation

L186D, L190D, L193D, I217K

Promoter

CMV

Available since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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ETV3_pet28a

Plasmid

#131646

Purpose

Bacterial expression of ETS gene ETV3

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV3 (ETV3 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJZC39

Plasmid

#62333

Purpose

sgRNA + 1x PP7 with mCherry for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 1x PP7
mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-myc NES-mCE(K294A) NLS-Flag

Plasmid

#82469

Purpose

Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme, inactive form

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

mRNA capping enzyme (Rngtt Mouse)

Use

Tags

Flag and Myc

Expression

Mammalian

Mutation

changed Lysine 294 to Alanine

Promoter

CMV

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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