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Showing: 8981 - 9000 of 14641 results

  8. pLenti GW V5 Eco/Dam hum LaminB1

    Plasmid
    #182671
    Purpose
    Eukaryotic expression vector with E.Coli Dam methylation fused to human Lamin B1. Used in DamID experiments to methylate DNA that interacts in the proximity of Lamin B1.
    Depositor
    Bas van Steensel
    Article
    Guelen et al Nature. 2008 Jun 12;453(7197):948-51. do
    Insert
    Lamin B1 (LMNB1 Human)
    Use
    Lentiviral
    Tags
    E. Coli Dam and V5
    Expression
    Mammalian
    Mutation
    Promoter
    HSP
    Available since
    April 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    182671-plasmid-map-sequence-id-358368

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  10. ACBE

    Plasmid
    #204607
    Purpose
    Mammalian expression of adenine-to-cytosine base editor ACBE
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103
    Insert
    bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)
    Promoter
    Available since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204607-plasmid-map-sequence-id-404719

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  12. AXBEv2

    Plasmid
    #204606
    Purpose
    Mammalian expression of evolved adenine transversion base editor AXBEv2
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103
    Insert
    bpNLS-TadA-8e-nSpCas9-mAAG (R165E/Y179F)-bpNLS-P2A-GFP
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)
    Promoter
    Available since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204606-plasmid-map-sequence-id-404720

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  14. VARP GFP-pLXIN

    Plasmid
    #62950
    Purpose
    full length human VARP with C-terminal EGFP tag cloned into pLXIN
    Depositor
    Paul Luzio
    Article
    Hesketh et al Dev Cell. 2014 Jun 9;29(5):591-606. doi:
    Insert
    VARP (ANKRD27 Human)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    silent mutations of amino acids 422-428 to produc…
    Promoter
    LTR
    Available since
    May 28, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. Cas12Max

    Plasmid
    #195334
    Purpose
    vector for encoding a human codon-optimized Cas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized Cas12Max
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    N243R
    Promoter
    CBh, CMV, hU6
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    195334-plasmid-map-sequence-id-383108

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Showing: 8981 - 9000 of 14641 results