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pAAVS1-P-CAG-GFPluc2

Plasmid

#80493

Purpose

donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP-luciferase fusion protein expression

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

GFPluc2

Use

Donor vector (human)

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_1_001

Plasmid

#167347

Purpose

ddPCR gating control for droplets containing all 5 targets (2 in pol)

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol_gag_tat_env

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-HA-hM3D(Gq)-IRES-mCitrine

Plasmid

#50466

Purpose

Gq-coupled hM3D-IRES-mCitrine under the control of CaMKIIa promoter

Depositor

Bryan Roth

Insert

hM3D(Gq)-IRES-mCitrine (CHRM3 Human)

Use

AAV

Tags

HA

Expression

Mutation

See supplemental documents for DREADD mutations

Promoter

CaMKIIa

Available since

April 16, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-JMJD2B

Plasmid

#24181

Purpose

Mammalian expression of human JMJD2B with HA tag

Depositor

Kristian Helin

Article

Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Insert

JMJD2B (KDM4B Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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V72 ELP

Plasmid

#68938

Purpose

Elastin-like Polypeptide (VPGxG) with 72 repeats containing Valine guest residue

Depositor

Andrew MacKay

Article

Dhandhukia et al Biomacromolecules. 2013 Apr 8;14(4):976-

Insert

V72 ELP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pBS185 CMV-Cre

Plasmid

#11916

Purpose

Mammalian expression of Cre recombinase driven by the human cytomegalovirus IE promoter

Depositor

Brian Sauer

Article

Sauer et al New Biol. 1990 May . 2(5):441-9.

Insert

cre (cre bacteriophage P1)

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pSDMA58

Plasmid

#67942

Purpose

Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site, a small gene-free region. Contains HYG resistance marker.

Depositor

James Fraser

Article

Arras et al PLoS One. 2015 Apr 9;10(4):e0122916. doi

Type

Empty backbone

Use

Cryptococcal complementation vector

Tags

Expression

Mutation

Promoter

ACT1 for Cryptococcal marker

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.eGFP.2A.wtTau

Plasmid

#140424

Purpose

AAV.CBA.eGFP.2A.wtTau propagation reporter WT-2N4R-hTau

Depositor

Bradley Hyman

Article

Wegmann et al Sci Adv. 2019 Jun 26;5(6):eaaw6404. doi:

Insert

eGFP-2A-Tau (MAPT Human, Synthetic)

Use

AAV

Tags

eGFP

Expression

Mutation

Promoter

CBA

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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FUGW-Golgi-TurboID

Plasmid

#200964

Purpose

3rd gen lentiviral plasmid for expressing golgi localized TurboID with EGFP

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

Golgi-TurboID

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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DH10GFP

Bacterial Strain

#109392

Purpose

DH10B with a "capacity monitor" inserted in the lambda phage integration site.

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Bacterial Resistance

Kanamycin

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only
SFFV-IKZF3-CTERM-GFP

Plasmid

#185769

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

William Sellers, Alessandra Ianari

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, IKZF3 minidegron

Expression

Mutation

none

Promoter

SFFV

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pmCherry-NLS

Plasmid

#39319

Depositor

Martin Offterdinger

Article

Micutkova et al Int J Cancer. 2012 Apr 1;130(7):1544-57.

Insert

mCherry-3xNLS

Use

Tags

3xSV40LT NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only

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pCAG-DsRed2

Plasmid

#15777

Depositor

Edward Callaway

Article

Wickersham et al Neuron. 2007 Mar 1. 53(5):639-47.

Insert

DsRed2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pLBH531_MBP-Cas14a1 expression

Plasmid

#112500

Purpose

Expresses MBP fused to Cas14a1

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14a1

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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Halo-Rab6A

Plasmid

#190171

Purpose

Expresses HaloTag-Rab6A in mammalian cells.

Depositor

Anna Akhmanova

Article

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Insert

HaloTag-Rab6A

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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8xNFAT-ZsG-mCD8

Plasmid

#162747

Purpose

NFAT-driven ZsGreen-1 reporter gene with mouse CD8 gene

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

8x of NFAT binding motif followed by ZsGreen-1 fluorescent protein gene

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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EasyCloneYALI- CRISPR/Cas9 Set

Plasmid Kit

#1000000141

Purpose

Contains plasmids for genome editing of Y. lipolytica via CRISPR/Cas9

Depositor

Irina Borodina

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only
pTubulin-miRFP670nano3

Plasmid

#184666

Purpose

Human alpha-tubulin labelled with near-infrared fluorescent protein miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

miRFP670nano3-human alpha-tubulin

Use

Tags

miRFP670nano3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-LKB1

Plasmid

#8590

Depositor

Lewis Cantley

Article

Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

Insert

LKB1 (STK11 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.PI.EGFP.WPRE.bGH (AAV1)

Viral Prep

#105530-AAV1

Purpose

Ready-to-use AAV1 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA. CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.

Depositor

James M. Wilson

Promoter

CMV

Tags

EGFP

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only