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Plasmid#132745PurposeT7 RNAP-driven expression of second-generation toehold repressor index 2 trigger RNADepositorInsertToeRepG2_N02_trigger
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS838 TDP43 RRM-GFP
Plasmid#98249PurposeTDP43 RRM-GFP reporter constructDepositorInsertTARDBP (TARDBP Human)
UseTagsInternal GFP to replace RRM domainsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0402
Plasmid#91009PurposeModule A, Promoter: AtUbi10, Gene: AtCas9_dead (D10A + H840A), Terminator: HSPDepositorInsertAtCas9_dead (D10A + H840A)
UseCRISPRTagsExpressionMutationD10A, H840APromoterAtUbi10Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
GWF297
Plasmid#133612PurposeTET3G-2xMS2(wt+f6), NLS-MCP-ANR-ANR-P2a-BFPDepositorInsertTET3G-2xMS2(wt+f6), MCP-ANR-ANR, BFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
GWF180
Plasmid#133607PurposeCAG-DNCR2-VPR-IRES-Puro-WPRE-SV40PA-PGK-NS3aH1-tagBFP-SpdCas9DepositorInsertDNCR2-VPR, NS3aH1-tagBFP-SpdCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-Syk-tSH2-PM
Plasmid#111271Purposeexpress murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation (Syk Mouse)
UseTagsExpressionBacterialMutationY130E mutation to mimic phosphorylationPromoterT7 promoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYZ_3WJrep_N20_switch
Plasmid#132722PurposeT7 RNAP-driven expression of 3WJ repressor index 20 switch RNA with a GFP-ASV reporterDepositorInsert3WJ_N20_switch
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYZ_3WJrep_N20_trigger
Plasmid#132726PurposeT7 RNAP-driven expression of 3WJ repressor index 20 trigger RNADepositorInsert3WJ_N20_trigger
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C2813
Plasmid#91092PurposeModule C, Promoter: PvUbi2, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9DepositorInsertCsy4 (+ BaeI site for GT donor cloning)
UseCRISPRTagsExpressionMutationPromoterPvUbi2Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B2517
Plasmid#91074PurposeModule B, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol IIIDepositorInsertEsp3I ccdb cassette for single gRNA spacer cloning
UseCRISPRTagsExpressionMutationPromoterTaU3Available sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAtCas9-D10A_2
Plasmid#91160PurposeCas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:npt IIDepositorInsertAtCas9_D10A (nickase)
UseCRISPRTagsExpressionPlantMutationD10APromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0601
Plasmid#91016PurposeModule A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_D10A nickase, Terminator: HSPDepositorInsertCsy4-P2A-AtCas9_D10A nickase
UseCRISPRTagsExpressionMutationD10APromoter35SAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJIT163-eAFID-3
Plasmid#167360PurposeFor inducing predictable deletions in protoplasts and wheat plantsDepositorInsertA3Bctd-Cas9-UDG-T2A-AP lyase
UseTagsExpressionPlantMutationWTPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTC365
Plasmid#91214Purposeprotoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, tRNA processing)DepositorInsert2 gRNAs targeting tomato ARF8A
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C2806
Plasmid#91091PurposeModule C, Promoter: FMV 34S, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9DepositorInsertCsy4 (+ BaeI site for GT donor cloning)
UseCRISPRTagsExpressionMutationPromoterFMV 34SAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pH-AFID-3
Plasmid#167361PurposeFor inducing predictable deletions in rice plantsDepositorInsertA3A-Cas9-UDG-T2A-AP lyase
UseTagsExpressionPlantMutationWTPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAtCas9_1
Plasmid#91156PurposeCas9 only expression, Cas9 Type: AtCas9, Plant Selection: 2x35S:hpt IIDepositorInsertAtCas9
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYZ_3WJrep_N10_trigger
Plasmid#132728PurposeT7 RNAP-driven expression of 3WJ repressor index 10 trigger RNADepositorInsert3WJ_N10_trigger
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYZ_3WJrep_N10_switch
Plasmid#132724PurposeT7 RNAP-driven expression of 3WJ repressor index 10 switch RNA with a GFP-ASV reporterDepositorInsert3WJ_N10_switch
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0801
Plasmid#91022PurposeModule A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSPDepositorInsertCsy4-P2A-AtCas9_dead (D10A + H840A)
UseCRISPRTagsExpressionMutationD10A, H840APromoter35SAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only