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Plasmid#163136PurposepLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the LppSP-OmpA surface expression anchor and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterP(rhaB)Available sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only
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pMC-1-EXOB-11
Plasmid#197688PurposePhytobrick (MoClo) Level 0 PartDepositorInsertEXOB enhancer-blocking insulator
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Zebrabow-GateDest
Plasmid#118221PurposeFor cloning promoter into a Zebrabow vector with Gateway reaction. Only compatible with single site Gateway. Not compatible with Tol2-kit.DepositorInsertBrainbow 1.0 L - ccdB survival cassette
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-Bin1b
Plasmid#109509PurposeMuscle specific zebrafish expression of mKate2 fused to Bin1bDepositorInsertBin1b (bin1b Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
LB701: pMVP/PB/mCherry-DEST
Plasmid#121871PurposepMVP destination vector, empty PiggyBac transposon vector backbone w/ mCherry selection cassetteDepositorTypeEmpty backboneUsePiggybac transposon, pmvp gateway destination vec…TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
JN302: pMVP/SB/Puro-DEST
Plasmid#121865PurposepMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Puro selection cassetteDepositorTypeEmpty backboneUseSleeping beauty transposon, pmvp gateway destinat…TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLOVE-Nmyc
Plasmid#15951DepositorInsertv-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (Mycn Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationThe recombination reaction required to clone &quo…PromoterAvailable sinceNov. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
LB901: pMVP/PB/Blast-DEST
Plasmid#121873PurposepMVP destination vector, empty PiggyBac transposon vector backbone w/ Blast selection cassetteDepositorTypeEmpty backboneUsePiggybac transposon, pmvp gateway destination vec…TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-FAPP1
Plasmid#109503PurposeMuscle specific zebrafish expression of mKate2 fused to the PH domain from Human pleckstrin homology domain containing A3 (PLEKHA3). Binds PtdIns4PDepositorInsertactc1b-mKate2-FAPP1
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
LC301: pMVP/PB/Zeo-DEST
Plasmid#121877PurposepMVP destination vector, empty PiggyBac transposon vector backbone w/ Zeo selection cassetteDepositorTypeEmpty backboneUsePiggybac transposon, pmvp gateway destination vec…TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-2xFYVEhgs
Plasmid#109505PurposeMuscle specific zebrafish expression of mKate2 fused to mouse 2x PH domain from HGF regulated tyrosine kinase substrate (Hgs). Binds PtdIns3PDepositorInsertactc1b-mKate2-2xFYVEhgs
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Kp-1
Plasmid#163143PurposepLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-MF pre-sequence and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAOX1Available sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
LC101: pMVP/PB/Neo-DEST
Plasmid#121875PurposepMVP destination vector, empty PiggyBac transposon vector backbone w/ Neo selection cassetteDepositorTypeEmpty backboneUsePiggybac transposon, pmvp gateway destination vec…TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDD315 (mTurquoise2^SEC^2xHA)
Plasmid#73343PurposemTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology armsDepositorInsertmTurquoise2-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags2xHA and C. elegans codon-optimized mTurquoise2ExpressionWormMutationPromoterAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOCC304
Plasmid#118897Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with TEV and C-terminal HIS6, cleavable with TEVDepositorInsertNcoI-TwinStrep-MBP-TEV-NotI-ccdB-AscI-TEV-HIS6-stop-HindIII cassette
UseTagsHIS6, cleavable with TEVprotease and TwinStrep-MB…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
LC001: pMVP/PB/Hygro-DEST
Plasmid#121876PurposepMVP destination vector, empty PiggyBac transposon vector backbone w/ Hygro selection cassetteDepositorTypeEmpty backboneUsePiggybac transposon, pmvp gateway destination vec…TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IY601: pMVP/Lenti/Blast-DEST
Plasmid#121849PurposepMVP destination vector, empty Lentivirus vector backbone w/ Blast selection cassetteDepositorTypeEmpty backboneUseLentiviral; Pmvp gateway destination vectorTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
LC201: pMVP/PB/Puro-DEST
Plasmid#121874PurposepMVP destination vector, empty PiggyBac transposon vector backbone w/ Puro selection cassetteDepositorTypeEmpty backboneUsePiggybac transposon, pmvp gateway destination vec…TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC119
Plasmid#118884Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEVDepositorInsertNcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
UseTagsHIS6-MBP, cleavable with 3C protease and mGFP, cl…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC72
Plasmid#118866Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEVDepositorInsertNcoI-HIS6-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease, mGFP, cleavable…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only