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Plasmid#104221PurposeBacterial expression of WW domain #2 from WWP1DepositorInsertWWP1 WW domain #2 (WWP1 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
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Venus-30aa-Noxa-pEGFP-C1
Plasmid#166762PurposeTo increase the length of the linker between Venus and Noxa proteinsDepositorInsertNoxa (PMAIP1 Human)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Venus-30aa-Noxa-4E-pEGFP-C1
Plasmid#167529PurposeTo increase the length of the linker between Venus and Noxa-4E proteinsDepositorInsertPMA-induced protein 1 (PMAIP1 Human)
UseTagsVenusExpressionMammalianMutationC25E, L29E, F32E, L36EPromoterCMVAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
alpha3-nAChR shRNA (Alpha-3.29)
Plasmid#86655Purposeexpression of shRNA targeting CHRNA3DepositorInsertCHRNA3 (alpha3-nAChR) (CHRNA3 Human)
UseRNAiTagsExpressionMammalianMutationPromoterH1Available sinceJune 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
PCDF Bravo-PotH-OL1del
Plasmid#216749PurposeStudy the interaction of PotH in E. coli, specifically focusing on the role of the outer loop 1 (OL1) by examining the variant lacking OL1 in its interaction with exogenous peptides.DepositorInsertpotH (potH E. coli (Bacteria))
UseTags10x HisExpressionBacterialMutationOuter loop 1 (OL1) with the sequence of [FKISLAEM…PromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
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4ROH
Plasmid#64659PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertITCH:JMC081-G03:C229184 (ITCH Human)
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 ITCH WW domain #3
Plasmid#104218PurposeBacterial expression of WW domain #3 from ITCHDepositorInsertITCH WW domain #3 (ITCH Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 ITCH WW domain #4
Plasmid#104219PurposeBacterial expression of WW domain #4 from ITCHDepositorInsertITCH WW domain #4 (ITCH Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 ITCH WW domain #1
Plasmid#104216PurposeBacterial expression of WW domain #1 from ITCHDepositorInsertITCH WW domain #1 (ITCH Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
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mCherry-hALIX
Plasmid#21504DepositorInsertALIX (PDCD6IP Human)
UseTagsCherryExpressionBacterialMutationnonePromoterAvailable sinceSept. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-A20
Plasmid#22141DepositorInsertA20 (TNFAIP3 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCBFD1/HBFD1Bad
Plasmid#53364PurposeWill convert beta-carotene into astaxanthin in E. coli when expression of the hbfd1 cDNA is induced with arabinose. Use with pAC-BETA or pAC-BETAipi.DepositorInsertcbfd1
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGST2-ALIX (795-811)
Plasmid#21502DepositorInsertALIX (PDCD6IP Human)
UseTagsGSTExpressionBacterialMutationnonePromoterAvailable sinceSept. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-A20 (OTU, aa 1-366)
Plasmid#61584PurposeExpresses human A20 (OTU domain) in E. coli.DepositorInsertA20 (TNFAIP3 Human)
UseTagsGST-3CExpressionBacterialMutationDeleted aa 367-790.PromotertacAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGST2-Alix (360-702)
Plasmid#17639DepositorInsertAlix (360-702) (PDCD6IP Human)
UseTagsGSTExpressionBacterialMutationAlix V domainPromoterAvailable sinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
5C7M (ITCH)
Plasmid#69966PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertITCH:SDC094-D06:C29524 (ITCH Human)
UseTagsHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacOAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -