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Plasmid#213907PurposeBarcoded mariner transposon with kan selection casette, and modified terminal sequences - barcodes can be added via FseI-SbfI restriction sitesDepositorInsertmariner transposon with kan resistance and FseI-SbfI sites for barcode insertion
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
T1T/pUBQ10(mTurq->mScarlet)
Plasmid#195947PurposeTp901 target with pUBQ10 switching from mTurq to mScarletDepositorInserttRBCS-mScarlet-attP_Tp901-pUBQ10-attB_Tp901-mTurq-tUBQ10
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
T1B/pUBQ10(mTurq->mScarlet)
Plasmid#195946PurposeBxb1 target with pUBQ10 switching from mTurq to mScarletDepositorInserttRBCS-mScarlet-attP_Bxb1-pUBQ10-attB_Bxb1-mTurq-tUBQ10
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTet-IRES-EGFP
Plasmid#64238PurposeLentiviral, doxycycline-inducible system to express transgene of interest and EGFP.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterpTetAvailable sinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMJA284= pSin-Pur-MCS-Puro
Plasmid#116877PurposeLentiviral transfer vector containing a multible cloning siteDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-csg-amylase
Plasmid#166859PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to α-amylase via a flexible linker. Derived from https://www.addgene.org/35363.DepositorInsertcsgBACEFG, with α-amylase fused to CsgA via flexible linker
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL0R_lacZ
Plasmid#202095PurposeReceiver cloning vector for making level 0 parts. Contains lacZ for blue/white screening.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pT7CFE1-Nhis-GST-CHA-YTH domain (YTHDC1, 317-532)
Plasmid#102277PurposeFor expression of His-GST-tagged human YTHDC1 YTH domain using mammalian cell-free protein expression system (1-step human high-yield mini IVT kit, cat. #88890, Thermo Fisher Scientific)DepositorInsertYTH domain of human YTHDC1 (317-532) (YTHDC1 Human)
UseFor mammalian cell-free protein expression system…TagsGST, HA, and HISExpressionMutationPromoterT7Available sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCA_e-2
Plasmid#202097PurposeReceiver cloning vector for even-2 level constructs. Contains lacZ for blue/white screening.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBbB8k-csg-NbGFP
Plasmid#166858PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a GFP-specific nanobody via a flexible linker. Derived from https://www.addgene.org/35363/.DepositorInsertcsgBACEFG, with GFP-specific nanobody fused to CsgA via flexible linker
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
M13-kmR
Plasmid#206861PurposeM13KE from NEB modified with kanamycin resistance gene and NotI restriction site on g3p ( M13KE-NotI-Kan)DepositorInsertKanamycin resistance gene
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_DE_mTurquoise2
Plasmid#202155PurposeLevel 0 plasmid containing a mTurquoise2 fluorescent protein with DE overhangs used to build a level 1 construct.DepositorInsertmTurquoise2 gene
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCA_e-4
Plasmid#202099PurposeReceiver cloning vector for even-4 level constructs. Contains lacZ for blue/white screening.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCA_e-3
Plasmid#202098PurposeReceiver cloning vector for even-3 level constructs. Contains lacZ for blue/white screening.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCA_e-1
Plasmid#202096PurposeReceiver cloning vector for even-1 level constructs. Contains lacZ for blue/white screening.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCA_o-4
Plasmid#202094PurposeReceiver cloning vector for odd-4 level constructs. Contains lacZ for blue/white screening.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCA_o-3
Plasmid#202093PurposeReceiver cloning vector for odd-3 level constructs. Contains lacZ for blue/white screening.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCA_o-2
Plasmid#202092PurposeReceiver cloning vector for odd-2 level constructs. Contains lacZ for blue/white screening.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCA_o-1
Plasmid#202091PurposeReceiver cloning vector for odd-1 level constructs. Contains lacZ for blue/white screening.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_EF-PtBle
Plasmid#202180PurposeLevel 0 plasmid containing a phleomycin antibiotic cassette (fcpF promoter) with EF overhangs used to build a level 1 construct.DepositorInsertShBle expression cassette
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits