Skip to main content
Addgene
Showing: 881 - 900 of 3910 results

  2. pCS2+-Pard3-EGFP-PIF6

    Plasmid
    #154914
    Purpose
    Full length zebrafish Pard3 (Gene ID: 403050), tagged with EGFP fluorescent protein and Arabidopsis PIF6 (as in construct Addgene ID 154913).
    Depositor
    Clare Buckley, Jonathan Clarke
    Article
    Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:
    Insert
    par-3 family cell polarity regulator alpha, b (pard3ab Zebrafish)
    Use
    Xenopus/avian/zebrafish
    Tags
    1-100 amino acids of Arabidopsis PIF6 and EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    154914-plasmid-map-sequence-id-308721

    Enlarge

  4. pLX304-YAP1 _60-89

    Plasmid
    #59144
    Purpose
    YAP1 mutants that harbored a deletion of amino acide at 60-89 position, which disrupts binding to TEAD transcription factors
    Depositor
    William Hahn
    Article
    Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.
    Insert
    YAP1 (YAP1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    aa60-89 deleted
    Promoter
    CMV
    Available since
    Aug. 19, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. SnapTag-Binder

    Plasmid
    #179142
    Purpose
    Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).
    Depositor
    Klaus Hahn
    Article
    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.
    Insert
    SspB
    Use
    Tags
    SNAP-tag
    Expression
    Mammalian
    Mutation
    Y11K/A15E, residue numbering from pdb: 1ou9
    Promoter
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    179142-plasmid-map-sequence-id-353405

    Enlarge

  7. pLX304-YAP1 _TA

    Plasmid
    #59143
    Purpose
    YAP1 mutants that harbor a deletion of the transcriptional activation domain
    Depositor
    William Hahn
    Article
    Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.
    Insert
    YAP1 (YAP1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    TA (transactivation) domain deleted; aa291-497
    Promoter
    CMV
    Available since
    Oct. 23, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. scADGFP

    Plasmid
    #114434
    Purpose
    Epilepsy promoter enhanced with Arc elements driving GFP with SV40E polyA
    Depositor
    Edward Perez-Reyes
    Article
    Burke et al Int J Mol Sci. 2023 Sep 23;24(19):14467.
    Insert
    Green fluorescent protein
    Use
    AAV
    Tags
    ER export sequence FCYENEV
    Expression
    Mammalian
    Mutation
    Promoter
    Novel synthetic activity-dependent promoter
    Available since
    Sept. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    114434-plasmid-map-sequence-id-225661

    Enlarge

  9. pRMC2-sec:msfGFP

    Plasmid
    #194914
    Purpose
    Tetracycline inducible expression of msfGFP fused to Sec signal peptide in Staphylococci
    Depositor
    Rikke Meyer
    Article
    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.
    Insert
    Shine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
    Use
    Tetracycline-inducible expression vector for stapā€¦
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    194914-plasmid-map-sequence-id-384625

    Enlarge

  11. EGFP-uTEV1-MAVS-mTA4

    Plasmid
    #171002
    Purpose
    HiLITR protease with MAVS [Y536delinsFIVLI] C-terminal tmd targeting information (Mitochondria and ER insertion)
    Depositor
    Alice Ting
    Article
    Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:
    Insert
    EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI]
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    MAVS(tmd)[Y536delinsFIVLI]
    Promoter
    pTRE-tight (TetOn)
    Available since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    171002-plasmid-map-sequence-id-335829

    Enlarge

  19. pEasyG2_nat

    Plasmid
    #184914
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_nat recombines in vivo with a PCR product from pEasyG2_mic.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184914-plasmid-map-sequence-id-367949

    Enlarge

Showing: 881 - 900 of 3910 results