Addgene

We narrowed to 4,567 results for: 027

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N85S EPHA3 pcDNA3.1

Plasmid

#102741

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Use

Tags

myc-6xhis

Expression

Mammalian

Mutation

N85S (AAC > AGC)

Promoter

CMV

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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STK19 gRNA (BRDN0001145122)

Plasmid

#76027

Purpose

3rd generation lentiviral gRNA plasmid targeting human STK19

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

STK19 (STK19 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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I-CAAA

Plasmid

#43027

Depositor

Chad Cowan, Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Insert

I-CAAA

Use

Bacterial cloning

Tags

Expression

Mutation

Promoter

Available since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
pLV-H2B-Maroon-P2A-3xnls-Tq-Ca-FLITS

Plasmid

#145027

Purpose

Lentiviral vector for a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changes - co-expresses a nuclear marker

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

3xnls-Tq-Ca-FLITS

Use

Lentiviral

Tags

3xnls-Tq-Ca-FLITS and H2B-Maroon

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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TOM20*-SBP-GFP

Plasmid

#120173

Purpose

Expresses a chimera of the mitochondria-targeting signal of TOM20, SBP tag and GFP (fluorescent tag)

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

Mitochondrial import receptor subunit TOM20 homolog (TOMM20 Human)

Use

Tags

SBP and eGFP

Expression

Mammalian

Mutation

Truncated TOM20: 1-30 aa

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pMT-EGFP-Actin5c

Plasmid

#15312

Depositor

Ron Vale

Article

Rogers et al J Cell Biol. 2003 Sep 15. 162(6):1079-88

Insert

Actin5c (Act5C Fly)

Use

Tags

EGFP and His

Expression

Insect

Mutation

Promoter

Available since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only
mCh-KIF17*-strep

Plasmid

#120167

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF17-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 17 (KIF17 Human)

Use

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF17: 1-509 aa

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR207 SARS-CoV-2 NSP3 Cys857Ala

Plasmid

#154027

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP3

Use

Gateway-compatible entry vector, with mutagenized…

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

June 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLEX303-ARF6-mNeonGreen

Plasmid

#162027

Purpose

Expression of tagged ARF WT

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

ARF6 (ARF6 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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TagBFP2-FKBP-INPP5ED477N

Plasmid

#202738

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

TagBFP2-FKBP-INPP5ED477N (INPP5E, FKBP1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-SBP-VAP-A*

Plasmid

#120175

Purpose

Expresses a chimera of GFP (fluorescent tag), SBP tag and the C-terminal of ER-resident protein VAP-A

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

VAMP associated protein A (VAPA Human)

Use

Tags

EGFP and SBP

Expression

Mammalian

Mutation

Truncated VAP-A: 118-249

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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mCh-KIF13A*-strep

Plasmid

#120166

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF13A-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 13A (KIF13A Human)

Use

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF13A: 1-639 aa

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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EphA2 430-976 pcDNA3.1

Plasmid

#102738

Purpose

Expression of c terminal EphA2 in mammalian cells

Depositor

Jin Chen

Article

Amato et al Cancer Res. 2016 Jan 15;76(2):305-18. do

Insert

EphA2 N terminal (430-976aa) (Epha2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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mCh-KIF5A*-strep

Plasmid

#120163

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF5A-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 5A (Kif5a Mouse)

Use

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF5A: 1-559 aa

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC25A20

Plasmid

#132027

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A20 (SLC25A20 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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G30, Cdc20-mCherry-AID tagging

Plasmid

#130270

Purpose

Tagging the Saccharomyces cerevisiae Cdc20 gene with a mCherry and auxin-degron tag, via homologous recombination

Depositor

Matthias Heinemann

Article

Ozsezen et al Cell Syst. 2019 Oct 4. pii: S2405-4712(1

Insert

Cdc20/mCherry-AID(71-114)-tCYC1-NatMX (CDC20 Budding Yeast, Synthetic)

Use

Tags

AID(71-114) and mCherry

Expression

Yeast

Mutation

Promoter

Native promoter

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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SRF6 B2_pECIA2

Plasmid

#115027

Purpose

Bait vector SRF6 B2_pECIA2 should be used with prey vector SRF6 B2_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G53730

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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EphA2 1-427 pcDNA3.1

Plasmid

#102737

Purpose

Expression of N terminal EphA2 in mammalian cells

Depositor

Jin Chen

Article

Amato et al Cancer Res. 2016 Jan 15;76(2):305-18. do

Insert

EphA2 N terminal (1-427aa) (Epha2 Mouse)

Use

Tags

6xHis and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pET-22b(+)_PelB-BN0034-5-FLAG-His

Plasmid

#218027

Purpose

RESOLUTE SLC binder expression plasmid

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN0034-5

Use

Tags

1xFLAG 10xHis and PelB

Expression

Bacterial

Mutation

Promoter

Available since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA T7-Gnat1 Mut-3UTR

Plasmid

#184027

Purpose

Expresses mouse Gnat1 protein with N-terminal T7 tag from cDNA that contains the mutant 3'-UTR where the TAG binding sites for MSI1 are mutated to TGA

Depositor

Peter Stoilov

Article

Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

Insert

Gnat1 (Gnat1 Mouse)

Use

Tags

T7

Expression

Mammalian

Mutation

TAG sites in the 3'-UTR are mutated to TGA

Promoter

CMV

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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