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pAcrF1
Plasmid#89233PurposePlasmid contains the gene for anti-CRISPR protein AcrF1 (gene 35 from bacteriophage JBD30), with N-terminal 6his tag and TEV protease cleavage siteDepositorInsertAcrF1
UseTags6xHis-TEV and FLAGExpressionMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
NG1237 pCI-TPI-PTC160-4H
Plasmid#65804PurposeNMD reporter mRNADepositorInsertTPI (TPI1 Human)
UseTags4H (4 copies of short beta-globin 3'UTR for …ExpressionMammalianMutationpremature stop codon at 160PromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOmniBac zz TEV YBBR TRF2
Plasmid#185443PurposeExpresses human TRF2 with a zz affinity tag, YBBR labeling site, and TEV cleavage site in insect cellsDepositorInsertTRF2 (TERF2 Human)
UseTagsYBBR and ZZExpressionInsectMutationPromoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-V5-SBP-HsSmg6-del36-576-siRNAres_I
Plasmid#146553PurposeMammalian Expression of HsSmg6-del36-576-siRNAresDepositorInsertHsSmg6-del36-576-siRNAres (SMG6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pVL94 - pOp4:VND7
Plasmid#116016Purposedestination vector for GreenGate cloning method, "Effector line", crossed with a "Driver line" the GOI, here VND7, will be expressed upon Dex-inductionDepositorInsertpOp4:B-dummy:VND7:VP16:tUBQ10::BastaR
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FluoSTEP-EKAR
Plasmid#181858PurposeGenetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-EKAR
UseTagsmRuby2 and sfGFP(1-10)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX Lnk-SH2
Plasmid#46451DepositorInsertLnk (Sh2b3 Mouse)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 325-466PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
E1b-GFP-Tol2-Gateway dre SE-zic2a S
Plasmid#89388PurposeVector for reporter assay of one zebrafish super-enhancer regionDepositorInsertzic2a (zic2a Zebrafish)
UseTagsExpressionMutationPromoterAvailable sinceJune 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-AGM-TAN1 vpr
Plasmid#115819PurposeEncodes codon optimized 3xFLAG-SIVAGM Vpr (TAN1) (U58991) with repaired premature stop codon and puromycin resistance proteinDepositorInsertSIVAGM Vpr (TAN1)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAVsc.U6-sgFah.CMV/CB-EGFP
Plasmid#121508PurposeExpresses sgRNA targeting mouse Fah intron 7 (sgFah).DepositorInsertsgFah
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO HIS DNAJB6a
Plasmid#19556DepositorInsertDNAJB6a (DNAJB6 Human)
UseTagsHisExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA1 3XFlag ATXN1[15Q]-delAXH
Plasmid#33241DepositorInsertATXN1[2Q]-delAXH (ATXN1 Human)
UseTags3xflagExpressionMammalianMutationPromoterCMVAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMflT-o4
Plasmid#101312PurposeContains: complete oriC region of M. florum L1 (oriC4: rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA), colE1 rep origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.DepositorInsertoriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX PLCg2(NC)-SH2
Plasmid#46472DepositorInsertPLCg2(NC) (PLCG2 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 524-749PromoterTacAvailable sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
AcrIIC1X
Plasmid#128112PurposeAcrX expression in mammalian cells; AcrX is an engineered anti-CRISPR protein targeting S. aureus Cas9DepositorInsertAcrX (AcrIIC1 N3F/D15Q/A48I)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV10-Flag-Fbxl21
Plasmid#48752PurposeExpression of FLAG-tagged Fbxl21DepositorInsertFbxl21 (Fbxl21 Mouse)
UseTags3XFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only
