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Viral Prep#162378-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (#162378). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8m-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only
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p46Cpf1-OP2
Plasmid#98592PurposeProduces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli.DepositorInsertFnCpf1
UseCRISPRTagsExpressionBacterialMutationCodon optimized for E. coliPromoteraraBADAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7-gRNA
Plasmid#46759PurposegRNA cloning vector for in vitro transcription of target gRNADepositorInsertgRNA core
UseCRISPR; Zebrafish expressionTagsExpressionMutationPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FNLSNG-P2A-Puro
Plasmid#136900PurposeLentivirus for constitutive expression of FNLSNG in mammalian cells (codon optimized)DepositorInsertFNLSNG
UseLentiviralTagsFLAG-NLS and NLSExpressionMutationL1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T1337RPromoterEF1sAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRBC-sfGFP wild type
Plasmid#174075PurposePlasmid with p15a origin of replication expressing codon optimized superfolder GFP with C-terminal His6 tag, under T7 promoterDepositorInsertSuperfolder GFP
UseTagsHis-6ExpressionBacterialMutationPromoterT7Available sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG-RbpjEnR
Plasmid#160924Purposedominant-negative construct to inhibit Notch signalingDepositorInsertRbpjEnR
UseTagsExpressionMutationfused with an engrailed repressorPromoterAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn1-mClover3/CAAX
Plasmid#197591PurposeLentiviral transfer vector for the neuronal expression of membrane-bound mClover3 (good visualization of neuronal processes)DepositorInsertmClover3-CAAX
UseLentiviralTagsCAAXExpressionMutationPromoterhSynIAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
4xMS2-hTR
Plasmid#154940Purpose4xMS2 stem-loop-hTR-500 bp genomic sequenceDepositorInsert4xMS2 stem-loop-hTR-500 bp genomic sequence
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-mNeonGreen-WPRE
Plasmid#193341PurposeAAV-mediated expression of mNeonGreen under the TRE promoter in mammalian cellsDepositorInsertmNeonGreen
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGHYB
Plasmid#87447PurposePichia pastoris expression plasmidDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterGAPAvailable sinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBP-split-intein-Gal4[21-881]-C-int
Plasmid#199202PurposeGateway destination vector for enhancer-driven split-intein Gal4[21-881]C-int expressionDepositorInsertsplit-intein-Gal4[21-881]-C-int
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
Skylan-S-(GGGGS)x3-β-Actin
Plasmid#128938Purposefluorescnece reporter of β-Actin with Skylan-S. Express in mammalian cellsDepositorInsertSkylan-S
UseTagsβ-actinExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Kras-Src FRET biosensor
Plasmid#78302PurposeFRET biosensor. To monitor Src kinase activity in mammalian cells by FRETDepositorInsertSrc biosensor
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_MAPK1_WT
Plasmid#82145PurposeGateway Donor vector containing MAPK1 , part of the Target Accelerator Plasmid Collection.DepositorInsertMAPK1 (MAPK1 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-TVA-V5-RG
Plasmid#119743PurposeExpresses TVA, V5, and RG in a cre dependent manner.DepositorInsertTVA, V5, Rabies Glycoprotein
UseAAV and Cre/LoxTagsV5ExpressionMutationPromoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/FLAG/HA-DEST TNRC6B
Plasmid#19884DepositorInserttrinucleotide repeat containing 6B (TNRC6B Human)
UseTagsFLAG/HAExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-CRAF
Plasmid#51124Purposemammalian expression of wt CRAFDepositorInsertCRAF (RAF1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
LacZ deficient (lac operon deletion) BL21-Gold-dLac (DE3) strain
Bacterial Strain#99247PurposeLacZ deficient (lac operon deletion) BL21-Gold-dLac (DE3) strainDepositorBacterial ResistanceTetracyclineAvailable sinceNov. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Beclin1
Plasmid#21150DepositorInsertPcDNA3-Beclin1 (BECN1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-Tev
Plasmid#177142PurposeBacterial vector for expression of an N-terminal GST fusion of the Tev proteaseDepositorInsertTev
UseTagsGSTExpressionBacterialMutationPromoterTacAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only