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Plasmid#179427PurposeDual fluorescent reporter construct with 1.2kb lambda spacer, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integrationDepositorInsertTRE-cit-1.2kb-mch
UseTagsExpressionMammalianMutationPromoterpEF alpha, pRSVAvailable sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
S+Q111R
Plasmid#167173PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with Q111R mutation in Sf9 cellsDepositorInsertsATP1A1-S+Q111R
ATP1B1
UseTagsExpressionBacterial and InsectMutationChanged Q at 111 to RPromoterP10 and PHAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
S+N122D
Plasmid#167174PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with N122D mutation in Sf9 cellsDepositorInsertsATP1A1-S+N122D
ATP1B1
UseTagsExpressionBacterial and InsectMutationChanged N at 111 to DPromoterP10 and PHAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCLXSN(GFP)-myc-BioID-hp100
Plasmid#174738PurposeGene overexpressionDepositorInsertP100 (NFKB2 Human)
UseRetroviralTagsExpressionMutationWTPromoterAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-dre4
Plasmid#170440PurposeExpress drosophila dre4 in E.coli expression system. Generated from pDONR-dre4 by Gateway system. Used for custom antibody purification.DepositorInsertdre4 (dre4 Fly)
UseTags6xHis and V5ExpressionBacterialMutationDeleted amino acids 1-20PromoterT7Available sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGMC00008
Plasmid#166724PurposesgRNA cloning backboneDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
P-Ef1a-EGFP-T2A-RPL22-1xFlag
Plasmid#170323PurposeExpresses FLAG and EGFP tagged RPL22DepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV_eA3A T31A-NG-nCas9
Plasmid#163539PurposeMammalian CG-to-GC base editingDepositorInserteA3A T31A-NG-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)eA3A T31ANG (L111R, D1135V, G1218R, E…PromoterAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV_RBMX-eA3A-UdgX-HF-NG-nCas9
Plasmid#163550PurposeMammalian CG-to-GC base editingDepositorInsertRBMX-eA3A-UdgX-HF-NG-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…PromoterAvailable sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCRI003-pGEM-LS-Bar-PgpdA-dSpCas9-VPR-TtrpC-LS
Plasmid#140196PurposeChromosomal integration of PgpdA-dSpCas9-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi vector.DepositorInsertsdSpCas9-VPR
Bar
Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
UseCRISPR and Synthetic Biology; Fungal genome integ…TagsVPR (VP64-p65-Rta) , NLSExpressionMutationPromoterPgpdA and PtrpCAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 295 (pDONR-Dll4-F2-ETS#2-WT-8X)
Plasmid#156412PurposeGateway middle entry clone containing an 8X multimerized ETS#2 DNA binding site from the murine Dll4 intronic enhancerDepositorInsertmurine Dll4 F2- ETS site B/ site #2, 8X
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DDX43-FL-G84D
Plasmid#134577PurposeExpress human DDX43 mutant (full-length with G84D mutation) in E. coliDepositorInsertDDX43-G84D (DDX43 Human)
UseTagsHisExpressionBacterialMutationfull-length (G84D)PromoterT7Available sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+ Flag-RAP2A
Plasmid#128099PurposeExpresses RAP2A in mammalian cells in a G418-selectable plasmidDepositorInsertRAP2A (RAP2A Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCri-H6-TEV-GRAB
Plasmid#128498PurposeExpresses GRAB (from V34 to N181) (Streptococcus pyogenes serotype M1) in bacterial cells. With N-t H6x tag + TEV.DepositorInsertGRAB (Streptococcus pyogenes serotype M1) (grab Streptococcus pyogenes serotype M1)
UseTagsHis tag (6x)ExpressionBacterialMutationPromoterT7Available sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCri-H6-TEV-GRAB-STREP
Plasmid#128499PurposeExpresses GRAB (from V34 to N181) in bacterial cells. With N-t H6x tag + TEV + protein + STREP(2x) tag.DepositorInsertGRAB (V34-N181) (grab Streptococcus pyogenes serotype M1)
UseTagsHis tag (6x) and Strep tag (2x)ExpressionBacterialMutationPromoterT7Available sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJET1.2_Gli3_Sense
Plasmid#124439PurposePlasmid for sense in situ probe in vitro transcriptionDepositorInsertGLI-Kruppel family member GLI3 (Gli3 Mouse)
UseIn situ probeTagsExpressionMutationPromoterT7Available sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLAP-BUBR1-K795A
Plasmid#114061Purposetransfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purificationDepositorInsertBUBR1-K795A (BUB1B Human)
UseTagsYFP-TEV-S- Human LAPExpressionMammalianMutationC2823A and G2826A (siRNA-resistance), BUBR1-K795APromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
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